The microbial community diversity in Constructed Wetland System (CWS) plays a key role the removal of pollutants from waste water. An integrated functional CWS developed at Neela Hauz Biodiversity Park, Delhi was selected to assess composition and structure sludge sediment CWS, based on metagenomic approach using 16S rRNA genes. showed higher than both formed distinct clusters. taxonomic is represented by 6,731 OTUs distributed among 2 kingdoms, 103 phyla, 227 classes, 337 orders, 320...

Background Stabilization strategies adopted by proteins under extreme conditions are very complex and involve various kinds of interactions. Recent studies have shown that a large proportion their N- C-terminal elements in close contact suggested they play role protein folding stability. However, the biological significance this remains elusive. Methodology In present study, we investigate residue interaction using family 10 xylanase (BSX) with TIM-barrel structure shows stability high...

Recent epidemics in snap bean (Phaseolus vulgaris) characterized by virus-like symptoms prompted a survey of commercial fields for Alfalfa mosaic virus (AMV), Cucumber (CMV), and the Bean yellow (BYMV)/Clover vein (ClYVV) complex 2002 2003. Snap were either remote from or adjacent to alfalfa (Medicago sativa), putative source these viruses. sampled at bloom stage both years. Model-adjusted mean incidences infection AMV, BYMV/ClYVV, CMV 41.96, 6.56, 6.69%, respectively, alfalfa, 6.66, 6.38,...

Abstract Antheraea assamensis is reared on various species of the Lauraceae family from north‐east India for its distinctive cocoon silk. We demonstrate differential expression digestive trypsin and chymotrypsins in larvae feeding a primary host, Persea bombycina Kosterm., comparison to Litsea monopetala Roxb. using vitro proteolytic assays, zymogram analyses with proteinase inhibitors, restriction digestion RNA‐PCR amplicons quantitative real‐time PCR (RT‐PCR). Eight novel members serine...

Pieris brassicae L. is a serious pest of cultivated crucifers in several parts the world. Larvae P. also feed prolifically on garden nasturtium (Tropaeolum majus L., family Tropaeolaceae). Proteolytic digestion was studied larvae feeding multiple hosts. Fourth instars were collected from cauliflower fields before transfer onto detached, aerial tissues selected host plants lab. Variable levels midgut proteases detected fed different hosts using protein substrates (casein and recombinant RBCL...

A. assamensis is a phytophagous Lepidoptera from Northeast India reared on host trees of Lauraceae family for its characteristic cocoon silk. Source these cocoons are domesticated farm stocks that crash frequently and/or wild insect populations provide new cultures. The need to reduce dependence necessitates assessment genetic diversity in cultivated and populations. Molecular markers based PCR Inter-simple sequence repeats (ISSR) simple (SSR) were used with four insects eleven insects. Wild...

Understanding the mechanisms that govern protein stability under poly-extreme conditions continues to be a major challenge. Xylanase (BSX) from Bacillus sp. NG-27, which has TIM-barrel structure, shows optimum activity at high temperature and alkaline pH, is resistant denaturation by SDS degradation proteinase K. A comparative circular dichroism analysis was performed on native BSX recombinant (R-BSX) with just one additional methionine resulting start codon. The results of this revealed...

Rapid adaptive responses were evident from reciprocal host-plant switches on performance, digestive physiology and relative gene expression of gut serine proteases in larvae crucifer pest P . brassicae transferred cauliflower (CF, Brassica oleracea var. botrytis, family Brassicaceae) to an alternate host, garden nasturtium, (GN, Tropaeolum majus L., Tropaeolaceae) vice-versa under laboratory conditions. Estimation nutritional indices indicated that all instars tested consumed the least food...

Gene fragments encoding the large subunit (LS) of Rubisco (RBCL) were cloned from various species host plants phytophagous Lepidoptera and expressed as recombinant proteins in Escherichia coli . Recombinant RBCLs compared among each other along with casein native proteinaceous substrates for measuring total midgut protease activities fourth instar larvae Helicoverpa armigera feeding on casein, Pieris brassicae cauliflower, Antheraea assamensis Litsea monopetala Persea bombycina Cognate rRBCL...

BPH (brown planthopper) and WBPH (white backed are significant rice pests that often co-occur as sympatric species cause substantial yield loss. Despite their genetic similarities, different host-resistance genes confer resistance against these two hoppers. The defense mechanisms in complex, the molecular processes regulating responses remain largely unknown. This study used specific recombinant inbred lines (RILs) derived from a cross between varieties RP2068-18-3-5 (BPH- WBPH-resistant)...

The brown planthopper (BPH; Nilaparvata lugens ) is one of India’s most destructive pests rice. BPH, a monophagous migratory insect, reported from all major rice-growing ecosystems the country, capable traversing large distances and causing massive crop loss. A crucial step for developing viable management strategies understanding its population dynamics. Very few reliable markers are currently available to screen BPH populations their diversity. In current investigation, we developed...

ABSTRACT Diverse bacterial assemblages were identified in a large, open stormwater drain (vernacular: nalah) built decades ago densely populated suburb of Delhi, India. Illumina-based next-generation sequencing (NGS) 16S rRNA gene amplicons was conducted with metagenomic DNAs isolated from influent sewage water and sediment samples collected Sahibabad drain, which now carries domestic industrial wastes to downstream treatment plants. Results are discussed respect diversity adaptation unique...

Dietary change influenced the life-history traits, nutritional utilization, and midgut serine proteinases in larvae of domesticated polyphagous S. ricini , transferred from R. communis (common name: castor; family Euphorbiaceae; host plant implicated its domestication) to A. excelsa Indian tree heaven; Simaroubaceae; an ancestral wild Samia species). Significantly higher values for fecundity body weight were observed feeding on (Scr diet), they took less time reach pupation than insects...