A5028051756- Advanced Proteomics Techniques and Applications
- Ovarian cancer diagnosis and treatment
- interferon and immune responses
- Influenza Virus Research Studies
- RNA and protein synthesis mechanisms
- Herpesvirus Infections and Treatments
- Histone Deacetylase Inhibitors Research
- Advanced Biosensing Techniques and Applications
- Epigenetics and DNA Methylation
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- DNA Repair Mechanisms
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Protein Degradation and Inhibitors
- PARP inhibition in cancer therapy
University of Minnesota
2021-2024
University of Iowa
2021-2023
It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion; however, the role of host factors in genome assembly not completely understood. To evaluate significance cellular assembly, we generated a reporter virus carrying tetracysteine tag NP gene (NP-Tc virus) and assessed dynamics vRNP localization with components by fluorescence microscopy. At early time points, were preferentially exported to MTOC;...
Abstract Background Ovarian cancer is the most lethal gynecologic malignancy in women, and high-grade serous ovarian (HGSOC) common subtype. Currently, no clinical test has been approved by FDA to screen general population for cancer. This underscores critical need development of a robust methodology combined with novel technology detect diagnostic biomarkers HGSOC sera women. Targeted mass spectrometry (MS) can be used identify quantify specific peptides/proteins complex biological samples...
High-grade serous ovarian cancer (HGSOC) is the most lethal gynecologic malignancy in women. Its low survival rate attributed to late detection, relapse, and drug resistance. The lack of effective second-line therapeutics remains a significant challenge. There an opportunity incorporate use histone deacetylase inhibitors (HDACi) into HGSOC treatment. However, mechanism efficacy HDACi context BRCA-1/2 mutation status understudied. Therefore, we set out elucidate how perturb proteomic...
High-grade serous ovarian cancer (HGSOC) is the most common form of diagnosed in patients worldwide. Patients with BRCA1/2-mutated HGSOC have benefited from targeted treatments such as poly(ADP-ribose) polymerase inhibitors (PARPi). Despite initial success PARPi-based treatment regimens, approximately 70% relapse and 5-year survival rate remains at 30%. PARPi exhibit variable efficacy toxicity profiles. Furthermore, off-target effects PARP inhibition not yet been fully elucidated, warranting...
Abstract Background: Ovarian cancer is the most lethal gynecologic malignancy in women, and high-grade serous ovarian (HGSOC) common subtype. Currently, no clinical test has been approved by FDA for detection of general population. This underscores critical need development a robust methodology combined with novel technology to detect diagnostic biomarkers HGSOC sera women. Targeted mass spectrometry (MS) can be used identify quantify specific peptides/proteins complex biological samples...
Serum contains several proteins that are associated with disease-related processes. Mass spectrometry (MS)-based proteomics approaches greatly facilitate serum protein biomarker development. However, the proteome complexity presents a technical challenge for accurate, sensitive, and reproducible quantification of by MS. Thus, efficient sample preparation methods critical importance analyses. In this study, we evaluated performance two using sera from patients high-grade serous ovarian cancer...
Summary It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion; however, the role of host factors in genome assembly not completely understood. To evaluate significance cellular assembly, we generated a reporter virus carrying tetracysteine tag NP gene (NP-Tc virus) and assessed dynamics vRNP localization with components by fluorescence microscopy. At early time points, were preferentially exported to MTOC;...
ABSTRACT The herpes simplex virus pUL34 component of the nuclear egress complex functions in both and cell-to-cell spread. A R158A/R161A double substitution has been shown to strongly inhibit Selection for extragenic suppressors UL34 on background a that expresses monomeric red fluorescent protein-tagged pUL35 yielded two viruses , SUP7 SUP9, replicated spread much better than parental virus. carried unique common genetic changes. most striking change was large rearrangement US region...