A5101759637- Lipid metabolism and biosynthesis
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Biochemical Acid Research Studies
- Yersinia bacterium, plague, ectoparasites research
- Species Distribution and Climate Change
- Plant Gene Expression Analysis
- Microbial Natural Products and Biosynthesis
- Environmental DNA in Biodiversity Studies
- Plant responses to water stress
- Microbial Metabolism and Applications
- Bat Biology and Ecology Studies
Iowa State University
2012-2020
Tianjin Nankai Hospital
2018
Jilin University
2018
U.S. National Science Foundation
2017
United States Army
2015
U.S. Army Engineer Research and Development Center
2015
Metabolomics is the methodology that identifies and measures global pools of small molecules (of less than about 1,000 Daltons) a biological sample, which are collectively called metabolome. can therefore reveal metabolic outcome genetic or environmental perturbation regulatory network, thus provide insights into structure regulation network. Because chemical complexity metabolome limitations associated with individual analytical platforms for determining metabolome, it currently difficult...
Malonyl-CoA is a key intermediate in number of metabolic processes associated with its role as substrate acylation and condensation reactions. These types reactions occur plastids, the cytosol mitochondria, although carboxylation acetyl-CoA known mechanism for generating distinct plastidial cytosolic pools, origin mitochondrial malonyl-CoA pool still unclear. In this study we demonstrate that synthetase encoded by Arabidopsis AAE13 (AT3G16170) gene localized both mitochondria. isoforms are...
Plant fatty acid biosynthesis occurs in both plastids and mitochondria. Here, we report the identification characterization of Arabidopsis (Arabidopsis thaliana) genes encoding three enzymes shared between mitochondria- plastid-localized type II synthase systems (mtFAS ptFAS, respectively). Two these enzymes, β-ketoacyl-acyl carrier protein (ACP) reductase enoyl-ACP reductase, catalyze two reactions that constitute core four-reaction cycle FAS system, which iteratively elongates acyl chain...
Acyl carrier protein (ACP) is a highly conserved cofactor that required by Type II fatty acid synthases (FASs). Here, we demonstrate up to three mitochondrial ACP (mtACP) isoforms support the Arabidopsis (Arabidopsis thaliana) mitochondrially localized FAS. The physiological importance of mtACPs was evaluated characterizing single, double, and triple mutants. mtACP1 (At2g44620), mtACP2 (At1g65290), mtACP3 (At5g47630) single mutants showed no discernible morphological growth phenotype....
We report the characterization of Arabidopsis (Arabidopsis thaliana) 3-hydroxyacyl-acyl carrier protein dehydratase (mtHD) component mitochondrial fatty acid synthase (mtFAS) system, encoded by AT5G60335. The localization and catalytic capability mtHD were demonstrated with a green fluorescent transgenesis experiment in vivo complementation vitro enzymatic assays. RNA interference (RNAi) knockdown lines reduced expression exhibit traits typically associated mtFAS mutants, namely miniaturized...
In this study we report the molecular genetic characterization of Arabidopsis mitochondrial phosphopantetheinyl transferase (mtPPT), which catalyzes phosphopantetheinylation and thus activation acyl carrier protein (mtACP) fatty acid synthase (mtFAS). This catalytic capability purified mtPPT (encoded by AT3G11470) was directly demonstrated in an vitro assay that phosphopantetheinylated mature apo-mtACP isoforms. The localization AT3G11470-encoded proteins validated ability their N-terminal...
In an attempt to fill knowledge gaps relating genetic structure in the endangered gray bat (Myotis grisescens), we investigated geographic patterns multilocus microsatellite DNA (msDNA) genotypes and mitochondrial (mtDNA) haplotype frequencies across eight primary hibernacula. Isolation-by-distance (IBD) was absent msDNA data no bottlenecks were detected, with genotypic diversity (A R = 6.52, H o 0.64) overall differentiation (F ST 0.024, P < 0.001) being comparable other Myotis North...
An HPLC-MS/MS method was described for the quantitation of lovastatin and its main hydroxy acid metabolite in human plasma. Lovastatin detected positive ion mode, while negative mode a single injection, with simvastatin as internal standard, respectively. Plasma sample treated by one-step liquid-liquid extraction dichloromethane-diethyl ether (2:3 v/v). LC separation performed within 4 min on Zorbax Eclipse XDB-C8 column (5 μm, 4.6 × 150 mm I.D.) using 90% methanol-10% – 10 mmol/l ammonium...
ABSTRACT We report the identification and characterization of genes encoding three enzymes that are shared between mitochondrial plastidial-localized Type II fatty acid synthase systems (mtFAS ptFAS, respectively). Two these enzymes, β-ketoacyl-ACP reductase (pt/mtKR) enoyl-ACP (pt/mtER) catalyze two reactions constitute core, 4-reaction cycle FAS system, which iteratively elongate acyl-chain by 2-carbon atoms per cycle. The third enzyme, malonyl-CoA:ACP transacylase (pt/mtMCAT) catalyzes...