A5080759990- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
Emory University
2022-2024
Rapid antigen tests (RATs) have become an invaluable tool for combating the COVID-19 pandemic. However, concerns been raised regarding ability of existing RATs to effectively detect emerging SARS-CoV-2 variants. We compared performance 10 commercially available, emergency use authorized against Delta and Omicron variants using both individual patient serially diluted pooled clinical samples. The exhibited lower sensitivity samples when PCR cycle threshold (C
Upper respiratory samples for SARS-CoV-2 detection include the gold standard nasopharyngeal (NP) swab, and mid-turbinate (MT) nasal swabs, oropharyngeal (OP) saliva. Following emergence of omicron (B.1.1.529) variant, limited preliminary data suggest that OP swabs or saliva may be more sensitive than highlighting need to understand differences in viral load across different sites.MT, OP, were collected from symptomatic individuals presenting evaluation Atlanta, GA, January 2022. Longitudinal...
Rapid Antigen Tests (RAT) have become an invaluable tool for combating the COVID-19 pandemic. However, concerns been raised regarding ability of existing RATs to effectively detect emerging SARS-CoV-2 variants. We compared performance eight commercially available, emergency use authorized against Delta and Omicron variants using individual patient serially diluted pooled clinical samples. The exhibited lower sensitivity samples when PCR Cycle threshold (C
Abstract Background Nasopharyngeal qualitative reverse-transcription polymerase chain reaction (RT-PCR) is the gold standard for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, but it not practical or sufficient in every clinical scenario due to its inability distinguish active from resolved infection. Alternative adjunct testing may be needed guide isolation precautions and treatment patients admitted hospital. Methods We performed a single-center,...
Traditional cellular and live-virus methods for detection of SARS-CoV-2 neutralizing antibodies (nAbs) are labor- time-intensive, thus not suited routine use in the clinical lab to predict vaccine efficacy natural immune protection. Here, we report development validation a rapid, high throughput method measuring nAbs against native-like trimeric spike proteins. This assay uses blockade human angiotensin converting enzyme 2 (hACE-2) binding (BoAb) approach an automated digital immunoassay on...
Limited data highlight the need to understand differences in SARS-CoV-2 omicron (B.1.1.529) variant viral load between gold standard nasopharyngeal (NP) swab, mid-turbinate (MT)/anterior nasal swabs, oropharyngeal (OP) and saliva. MT, OP, saliva samples from symptomatic individuals Atlanta, GA, January 2022 longitudinal a small familial cohort were tested by both RT-PCR ultrasensitive antigen assays. Higher concentrations nares observed cohort, but dominant sample type was not found among 39...
The motivation for this work was the need to establish a predefined cutoff based on genome copies per ml (GE/ml) rather than Ct, which can vary depending laboratory and assay used. A GE/ml-based threshold necessary define what constituted 'low positives" samples that were included in data sets submitted FDA emergency use approval SARS-CoV-2 antigen tests.SARS-CoV-2, causal agent of global COVID-19 pandemic, made its appearance at end 2019 is still circulating population. pandemic led an...
Viability of saliva samples stored for longer than 28 days has not been reported in the literature. The COVID-19 pandemic spawned new research evaluating various sample types, thus large biobanks have started. Residual from university student surveillance testing were retested on SalivaDirect and compared with original RT-PCR (cycle threshold values) quantitative antigen values each month storage. We conclude that at -80°C are still viable detecting SARS-CoV-2 after 12 months storage,...