A5010816544- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Insect and Arachnid Ecology and Behavior
- Mycorrhizal Fungi and Plant Interactions
- Natural Antidiabetic Agents Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Insect-Plant Interactions and Control
- Phytochemicals and Antioxidant Activities
- Genetically Modified Organisms Research
- Seed and Plant Biochemistry
- Environmental Toxicology and Ecotoxicology
- Aquatic Invertebrate Ecology and Behavior
University of Missouri
2013-2023
Biological Control of Insects Research Laboratory
2017-2019
Agricultural Research Service
2017-2019
Providence College
2016
University of Nebraska–Lincoln
2013
Abstract Diabrotica virgifera LeConte, the western corn rootworm (WCR), is most destructive pest of maize in North America, and has recently spread across central Europe. Its subterranean larval stages are hard to reach with pesticides it evolved resistance conventional management practices. The application beneficial soil organisms being considered as a sustainable environmental friendly alternative. In previous study, combined wheat fields arbuscular mycorrhizal fungi, entomopathogenic...
The frequency of extended diapause in populations the northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae), was measured eastern Nebraska. Adult collections were made during late summer 2008 and 2009 from eight sites each year (seven consistent over years). Eggs obtained 12 to 20 females per site held on moist soil under appropriate temperature profiles facilitate egg survival, development, termination. Percentage hatch recorded after first second for...
Both an eCry3.1Ab-selected and paired control western corn rootworm, Diabrotica virgifera LeConte, colony were tested for adult longevity, egg oviposition, viability, larval development in order to evaluate the potential fitness costs associated with eCry3.1Ab resistance. Adult longevity experiments conducted by pairing virgin males females together plastic boxes supplied food, water, ovipositional medium observed survival time. Eggs also collected from once a week determine average...
Abstract Bioassays involving newly hatched larval insects can be limited by the larvae’s feeding state. Assays attempting to monitor mortality effects negatively affected starvation on larvae. Neonate western corn rootworms have significant reductions in viability if not provided food within 24 h post hatch. The recent development of an improved artificial diet for rootworm larvae provides a new bioassay type evaluating entomopathogenic nematodes that also makes testing arena easy observe....
Larval Diabrotica virgifera LeConte (Coleoptera: Chrysomelidae) were exposed to seven different entomopathogenic nematode species test their potential infectivity in a laboratory setting. Known D. virgifera-infecting Heterorhabditis bacteriophora Poinar, megidis Jackson & Klein, Steinernema feltiae Filipjev, and carpocapsae Weiser tested concerted experiment alongside diaprepesi Nguyen Duncan, riobrave Cabanillas, Poinar Raulston, Missouri wild-type H. which have not been previously on...
The northern corn rootworm, Diabrotica barberi Smith & Lawrence, has a univoltine life cycle that typically produces one generation year. When rearing the rootworm in laboratory, order to break diapause, it is necessary expose eggs five month cold period before raising temperature. By selective breeding of small fraction hatched without within 19-32 days post oviposition, we were able develop non-diapausing colony generations selection. Through selection, percentages adult emergence from egg...
Corn fields planted with plant-incorporated Bacillus thuringiensis (Bt) proteins must have a portion of the field non-Bt, isoline, plants that serve as refuge for susceptible insects. In Belt, seeds are now blended in bag Bt corn hybrids containing two or more toxins targeted toward western rootworm, Diabrotica virgifera LeConte. Syngenta's hybrid, Agrisure Duracade, eCry3.1Ab (event 5307) and mCry3a MIR604) rootworm-targeted were registered seed blend 2014. Western rootworm larval movement...
Two new insect colonies were created by separating virgin western corn rootworm, Diabrotica virgifera LeConte, males and females from both a selected laboratory colony that was being reared on eCry3.1Ab-expressing ( Zea mays L.) control its near-isoline corn. Females the paired with of vice versa to create female male (Sel♀) (Con♀). Both along their parental (eCry3.1Ab-selected control) evaluated in seedling assays. Larvae each also used diet toxicity experiments order determine LC 50 EC...
The northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae), is one of the most important insect pests in U.S. Corn Belt. Efforts to obtain eggs from wild rootworm populations using techniques developed for other species have been unsuccessful due lack oviposition. In 2016, we evaluated four oviposition media choice tests within each three female densities 30.5 × cm BugDorm cages. number laid per was significantly affected by density and interaction media, but...
Western, northern, Mexican, and southern corn rootworms (WCR, NCR, MCR, SCR) are serious pests. We evaluated host search behavior of these pests on six plant species using a video tracking system. After 5-min exposure to roots, behavioral parameters were automatically recorded used quantify the behavior. The was not observed for sorghum since no neonates survived after contacting roots. exposures all exhibited localized behaviors (i.e., shortening total distance traveled, lowering movement...
Abstract The northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae) is a major pest of maize in the United States Corn Belt. Recently, resistance to Bacillus thuringiensis (Bt) was reported North Dakota and increased use Bt hybrids could facilitate evolution other maize-producing states. In this study, susceptibility proteins evaluated wild D. populations from 8 fields collected 5 different states (Minnesota, Missouri, Nebraska, Iowa, Dakota). Field were...
The western corn rootworm (WCR), Diabrotica virgifera LeConte, is the most serious pest of maize in United States. In pursuit developing a diet free antibiotics for WCR, we characterized effects thermal exposure (50–141 °C) and length on quality WCRMO-2 measured by life history parameters larvae (weight, molting, survival) reared diet. Our results indicated that temperatures had non-linear performance diet, no impacts were observed time exposure. optimum temperature processing was 60 °C...
The western corn rootworm (WCR), Diabrotica vifgirera virgifera LeConte, (Coleoptera: Chrysomelidae) causes significant economic damage in production each year. Resistance to insecticides and transgenic with Bacillus thuringiensis (Bt), Berliner toxins have been reported throughout the United States Corn Belt. breeding programs for natural resistance against WCR larvae could potentially assist management. Root root regrowth of eight lines were evaluated field assays at three different...
Western corn rootworm, Diabrotica virgifera LeConte, became much easier to research with the development of a nondiapausing rootworm strain. In event that eggs cannot be used immediately researchers have been known delay egg hatch by storing at low temperatures. It is not well how this technique could affect or larval development, which alter results an experiment. To test for western were stored temperatures potential negative effects on and development. Eggs in either soil agar placed...