A5038082147- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Coral and Marine Ecosystems Studies
- Chromosomal and Genetic Variations
- Environmental Toxicology and Ecotoxicology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA and protein synthesis mechanisms
- Marine Toxins and Detection Methods
- Marine and fisheries research
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Aquatic Invertebrate Ecology and Behavior
- Environmental DNA in Biodiversity Studies
- Genetic diversity and population structure
- Sperm and Testicular Function
- Marine Bivalve and Aquaculture Studies
- RNA modifications and cancer
- Animal Genetics and Reproduction
- DNA Repair Mechanisms
- RNA Research and Splicing
- Marine Ecology and Invasive Species
- Insect behavior and control techniques
- Coastal wetland ecosystem dynamics
- Marine Sponges and Natural Products
Florida International University
2016-2025
Universidade da Coruña
2004-2013
University of Victoria
2005-2009
Abstract Histone variants are non-allelic protein isoforms that play key roles in diversifying chromatin structure. The known number of such has greatly increased recent years, but the lack naming conventions for them led to a variety styles, multiple synonyms and misleading homographs obscure variant relationships complicate database searches. We propose here unified nomenclature all five classes histones uses consistent flexible produce names informative readily searchable. builds on...
Abstract Background Within chromatin, the histone variant H2A.Z plays a role in many diverse nuclear processes including transcription, preventing spread of heterochromatin and epigenetic transcriptional memory. The molecular mechanisms how mediates its effects are not entirely understood. However, it is now known that has two protein isoforms vertebrates, H2A.Z-1 H2A.Z-2, which encoded by separate genes differ 3 amino acid residues. Results We report H2A.Z-2 expressed across wide range...
In the tephritids Ceratitis capitata and Bactrocera oleae, gene transformer acts as memory device for sex determination, via an auto-regulatory function; functional Tra protein is produced only in females. This paper investigates evolution of tra, which was characterised twelve tephritid species belonging to less extensively analysed genus Anastrepha. Our study provided following major conclusions. Firstly, mechanism used by this determination likely existed common ancestor Ceratitis,...
The histone H2A family encompasses the greatest number of core variants which replacement variant H2A.Z is currently one most heavily studied. No clear mechanism for functional variability that imparts to chromatin has yet been proposed. While past studies have referred generically as a single protein, in vertebrates it mixture two protein forms H2A.Z-1 (previously H2A.Z) and H2A.Z-2 H2A.F/Z or H2A.V) differ by three amino acids.We performed an extensive study on long-term evolution across...
Despite the identification of H2A.Bbd as a new vertebrate-specific replacement histone variant several years ago, and despite many in vitro structural characterizations using reconstituted chromatin complexes consisting this variant, existence cell its location has remained elusive. Here, we report that native form is present highly advanced spermiogenic fractions mammalian testis at time when histones are acetylated being replaced by protamines. It also nucleosomal fraction mature human...
Abstract Background In the tephritids Ceratitis , Bactrocera and Anastrepha gene transformer provides memory device for sex determination via its auto-regulation; only in females is functional Tra protein produced. To date, isolation characterisation of transformer-2 has been undertaken it shown that function required female-specific splicing doublesex pre-mRNA. It therefore participates auto-regulatory function. this work, eleven tephritid species belonging to less extensively analysed...
Abstract Nutrient pollution and thermal stress constitute two of the main drivers global change in coastal oceans. While different studies have addressed physiological effects ecological consequences these stressors corals, role acquired modifications coral epigenome during acclimatory adaptive responses remains unknown. The present work aims to address that gap by monitoring types epigenetic mechanisms, namely histone DNA methylation, a 7‐week‐long experiment which staghorn fragments (...
Age constitutes a critical parameter for the study of animal populations, providing information about development, environmental effects, survival, and reproduction. Unfortunately, age estimation is not only challenging in large, mobile legally protected species, but often involves invasive sampling methods. The present work investigates association between epigenetic modifications chronological small cetaceans. For that purpose, characterized DNA methylation at age-linked genes an...
Epigenetic modifications such as DNA methylation have been shown to participate in plastic responses environmental change a wide range of organisms, including scleractinian corals. Unfortunately, the current understanding links between signals, epigenetic modifications, and subsequent consequences for acclimatory phenotypic changes remain obscure. Such knowledge gap extends also dynamic nature changes, hampering our ability ascertain magnitude extent these under natural conditions. The...
Abstract The methyltransferase-like (METTL) proteins constitute a family of seven-beta-strand methyltransferases with S-adenosyl methionine-binding domains that modify DNA, RNA, and proteins. Methylation by METTL contributes to the epigenetic, in case RNA modifications, epitranscriptomic regulation variety biological processes. Despite their functional importance, most investigations substrates functions METTLs within metazoans have been restricted model vertebrate taxa. In present work, we...
Non-coding RNAs (ncRNAs) play critical regulatory roles in gene expression regulation that influences diverse biological processes response to environmental change. Yet their characterization non-model organisms, particularly sessile, benthic ecosystem engineers such as reef-building corals are sensitive climate change, remains limited. This study provides the first comprehensive analysis of ncRNA repertoire species from three ecologically important coral genera Moorea, French Polynesia:...
Family 32 carbohydrate-binding modules (CBM32s) are found in a diverse group of microorganisms, including archea, eubacteria, and fungi. Significantly, many members this family belong to plant animal pathogens where they likely play key role enzyme toxin targeting function. Indeed, ligand targets have been shown range from insoluble cell wall polysaccharides complex eukaryotic glycans. Besides potential direct involvement microbial pathogenesis, CBM32s also represent an important for the...
Histone variants play a critical role in chromatin structure and epigenetic regulation. These "deviant" proteins have been historically considered as the evolutionary descendants of ancestral canonical histones, helping specialize nucleosome during eukaryotic evolution. Such view is now challenged by 2 major observations: first, histones present extremely unique features not shared with any other genes; second, histone are widespread across many groups. The work further supports nature...