A5031102509- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Genetics, Aging, and Longevity in Model Organisms
- Nuclear Receptors and Signaling
- Neuroscience and Neural Engineering
- Pancreatic function and diabetes
- Mesenchymal stem cell research
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- Phagocytosis and Immune Regulation
Centre for Genomic Regulation
2014-2019
Vita-Salute San Raffaele University
2013-2014
Universitat Pompeu Fabra
2014
Direct lineage reprogramming through genetic-based strategies enables the conversion of differentiated somatic cells into functional neurons and distinct neuronal subtypes. Induced dopaminergic (iDA) can be generated by direct skin fibroblasts; however, their in vivo phenotypic properties remain incompletely understood, leaving impact on Parkinson's disease (PD) cell therapy modeling uncertain. Here, we determined that iDA retain a transgene-independent stable phenotype culture animal...
Current protocols for in vitro differentiation of human induced pluripotent stem cells (hiPSCs) to generate dopamine (DA) neurons are laborious and time-expensive. In order accelerate the overall process, we have established a fast protocol by expressing developmental transcription factors ASCL1, NURR1, LMX1A. With this method, were able mature functional dopaminergic as few 21 days, skipping all intermediate steps inducting selecting embryoid bodies rosette-neural precursors. Strikingly,...
Cyclic activation of the Wnt/β-catenin signaling pathway controls cell fusion-mediated somatic reprogramming. TCFs belong to a family transcription factors that, in complex with β-catenin, bind and transcriptionally regulate Wnt target genes. Here, we show that needs be off during early reprogramming phases mouse embryonic fibroblasts (MEFs) into iPSCs. In MEFs undergoing reprogramming, senescence genes are repressed mesenchymal-to-epithelial transition is favored. This correlated repressive...
Abstract Homotypic and heterotypic cell-to-cell fusion are key processes during development tissue regeneration. Nevertheless, aberrant cell can contribute to tumour initiation metastasis. Additionally, a form of cell-in-cell structure called entosis has been observed in several human tumours. Here we investigate interaction between mouse mesenchymal stem cells (MSCs) embryonic (ESCs). MSCs represent an important source adult since they have great potential for regenerative medicine, even...
Abstract Mouse embryonic stem cells (mESCs) are pluripotent and can differentiate into belonging to the three germ layers of embryo. However, mESC pluripotency genome stability be compromised in prolonged vitro culture conditions. Several factors control pluripotency, including Wnt/β-catenin signaling pathway, which is essential for differentiation proliferation. Here we show that activity pathway safeguards normal DNA methylation mESCs. The progressively silenced during passages this...
Imprinted genes control several cellular and metabolic processes in embryonic adult tissues. In particular, paternally expressed gene-3 (Peg3) is active the stem cell population during muscle neuronal lineage development. Here we have investigated role of Peg3 mouse cells (ESCs) process somatic reprogramming towards pluripotency. Our data show that knockdown increases expression pluripotency ESCs enhances efficiency both fibroblasts neural cells. Interestingly, observed altered activity...