A5004441101- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Neuroscience and Neuropharmacology Research
- Probiotics and Fermented Foods
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- ATP Synthase and ATPases Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Oral microbiology and periodontitis research
- Protein Kinase Regulation and GTPase Signaling
- Genetics and Neurodevelopmental Disorders
- Parasitic Diseases Research and Treatment
- Mitochondrial Function and Pathology
- Parasite Biology and Host Interactions
- Fungal and yeast genetics research
- Gut microbiota and health
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- Congenital heart defects research
- Cardiomyopathy and Myosin Studies
- RNA Research and Splicing
- Malaria Research and Control
University of Massachusetts Amherst
2020-2024
The Ohio State University
2018-2022
Boston University
2019
Candida albicans surface-attached biofilms such as those formed on intravenous catheters with direct access to the bloodstream often serve a nidus for continuous release of cells capable initiating new infectious foci. We previously reported that dispersed from biofilm are yeast originate top-most hyphal layers biofilm. Compared their planktonic counterparts, these dispersal displayed enhanced virulence-associated characteristics and drug resistance. However, little is known about molecular...
Structural and functional analyses suggest that the hub domain of CaMKII regulates kinase activity.
Infectious fungal species are often treated uniformly despite clear evidence of genotypic and phenotypic heterogeneity being widespread across strains. Identifying the genetic basis for this diversity is extremely challenging because tens or hundreds thousands variants that may distinguish two
Gene duplication facilitates functional diversification and provides greater phenotypic flexibility to an organism. Expanded gene families arise through repeated but the extent of divergence that accompanies each paralogous is generally unexplored because difficulty in isolating effects single family members. The telomere-associated (TLO) a remarkable example expansion, with 14 members more pathogenic Candida albicans relative two TLO genes closely-related species C. dubliniensis. encode...
Biofilms are organized communities of microbial cells that promote persistence among bacterial and fungal species. Biofilm formation by host-associated Candida species fungi occurs on both tissue surfaces implanted devices, contributing to host colonization disease. In C. albicans, biofilms built sequentially adherence yeast a surface, invasion into the substrate, aerial hyphal projections, secretion extracellular matrix. Measurement these biofilm-related phenotypes remains highly...
Abstract Subtelomeres are dynamic genomic regions shaped by elevated rates of recombination, mutation, and gene birth/death. These processes contribute to formation lineage-specific family expansions that commonly occupy subtelomeres across eukaryotes. Investigating the evolution subtelomeric families is complicated presence repetitive DNA high sequence similarity among members prevents accurate assembly from whole genome sequences. Here, we investigated telomere-associated (TLO) in Candida...
Candida species are common fungal commensals of humans colonizing the skin, mucosal surfaces, and gastrointestinal tract. Under certain conditions, can overgrow their natural niches resulting in debilitating infections as well life-threatening systemic infections, which a major focus investigation due to associated high mortality rates. Animal models disseminated infection exist for studying disease progression dissecting characteristics pathogenicity. Of these, Galleria mellonella waxworm...
Abstract Ca 2+ -calmodulin dependent protein kinase II (CaMKII) plays a central role in signaling throughout the body. Specifically hippocampus, CaMKII is required for learning and memory. encoded by four highly conserved genes vertebrates: α, β, γ, δ. AllCaMKIIs are comprised of domain, regulatory segment, variable linker region, hub domain responsible oligomerization. The differ primarily length composition due to extensive alternative splicing. Here, we unambiguously report heterogeneity...
Abstract Pseudomonas aeruginosa is a Gram-negative bacterium responsible for numerous human infections. Previously, novel antibiotic tolerant variants known as phoenix colonies well similar to viable but non-culturable (VBNC) were identified in response high concentrations of aminoglycosides. In this study, the mechanisms behind colony and VBNC-like emergence further explored using both whole genome sequencing RNA sequencing. Phoenix found have single nucleotide polymorphism (SNP) PA4673...
Abstract Candida albicans surface-attached biofilms are sites of amplification an infection through continuous discharge cells capable initiating new infectious foci. Yeast released from on intravenous catheters have direct access to the bloodstream. We previously reported that dispersed largely lateral yeast originate hyphal layers biofilm. Compared their planktonic counterparts, these biofilm-dispersed displayed enhanced virulence-associated gene expression and drug resistance. Little is...
Abstract Pseudomonas aeruginosa is a Gram-negative bacterium responsible for numerous human infections. Previously, novel antibiotic tolerant variants known as phoenix colonies well similar to viable but non-culturable (VBNC) were identified in response high concentrations of aminoglycosides. In this study, the mechanisms behind colony and VBNC-like emergence further explored using both whole genome sequencing RNA sequencing. Phoenix found have single nucleotide polymorphism (SNP) PA4673...
ABSTRACT Subtelomeres are dynamic genomic regions shaped by elevated rates of recombination, mutation, and gene birth/death. These processes contribute to formation lineage-specific family expansions that commonly occupy subtelomeres across eukaryotes. Investigating the evolution subtelomeric families is complicated presence repetitive DNA high sequence similarity among members prevents accurate assembly from whole genome sequences. Here we investigated te lo mere-associated ( TLO ) in...
ABSTRACT The human commensal and opportunistic fungal pathogen Candida albicans displays extensive genetic phenotypic variation across clinical isolates. Here, we performed RNA sequencing on 21 well-characterized isolates to examine how contributes gene expression differences, link these differences traits. C. adapts primarily through clonal evolution yet hierarchical clustering of profiles in this set did not reproduce their phylogenetic relationship. Strikingly, strain-specific was...