A PCR assay was developed for the diagnosis of invasive aspergillosis in immunocompromised patients. For this purpose, complete nucleotide sequences genes encoding 18S rRNA Aspergillus nidulans, terreus, niger, and flavus were elucidated aligned to fumigatus other clinically relevant prokaryotic eukaryotic microorganisms. Genus-specific could be identified V7 V9 region rRNA. By using hot-start PCR, Southern blot hybridization, restriction enzyme analysis, Aspergillus-specific -sensitive...

As a subclass of microplastics, tire and road wear particles have been recognized as source toxicants in the aquatic environment. Tire rubber contains chemicals associated with serious toxicological effects, especially some salmonid species, but not other fish species. Among these are polynuclear aromatic hydrocarbons (PAHs) 6PPD-Q. These detoxified excreted through enzymatic biotransformation, notably by cytochrome P-450 (CYP) isoforms UDP-glucuronosyltransferases (UGT). We tested...

Bivalves have been extensively utilized as sentinel biomonitoring species, organisms used to predict the extent and severity of environmental contamination. However, significant knowledge gaps remain regarding operationalization application indicator species for radioactive contaminants in marine environment. Dose-dependent organism responses with validated practical measurement protocols need be identified use within frameworks. Our study explored tissue-specific oxidative stress filtration...

Toxicity testing of whole sediments was conducted as part the Bremerhaven Workshop designed to test various methods (chemical and biological) for assessing status North Sea waters, biota.Six investigators from 4 countries were involved; laboratory after transporting field-collected distances varying tens miles thousands miles.Sediments tested 2 contamination gradients, one an abandoned drilling site, other mouth Elbe northwest across German Bight.Methods included 11 different tests (20...

Antidepressants have been found in measurable concentrations final treated wastewater effluent and receiving waters throughout the world. Studies shown that these are typically not overtly toxic, but psychotropic mode of action chemicals warrants examination their behavioral effects. Exposure hybrid striped bass to antidepressants fluoxetine or venlafaxine alone has cause decreased brain serotonin levels increased time capture prey at 1 2 orders magnitude higher than environmentally relevant...

Seafood mislabeling distorts the true abundance of fish in sea, defrauds consumers, and can also cause unwanted exposure to harmful pollutants. By combining genetic data with analyses total mercury content, we have investigated how species substitutions fishery-stock obscure contamination Patagonian toothfish (Dissostichus eleginoides), known as “Chilean sea bass”. show wide variation concentrations such that consumers may be exposed either acceptable or unacceptable levels depending on...

Polychlorinated biphenyls (PCBs) can be metabolized by cytochromes P450 to hydroxylated biotransformation products. In mammalian studies, some of the products have been shown strong inhibitors steroid sulfotransferases. As a part ongoing research into bioavailability environmental pollutants in catfish intestine, we investigated effects series PCBs (OH-PCBs) on two conjugating enzymes, phenol-type sulfotransferase and glucuronosyltransferase. We incubated cytosolic microsomal samples...

Abstract Concurrent with the increase in use of carbon nanotubes (CNTs) society is rise their introduction into environment. Carbon cause adverse effects themselves, and they have potential to adsorb contaminants such as polycyclic aromatic hydrocarbons (PAHs). Although CNTs a high adsorption capacity for PAHs these can co‐occur environment, few studies characterized bioavailability CNT‐adsorbed fish. The goal present study was characterize fluoranthene adsorbed suspended multiwalled‐carbon...

Abstract Sediments from Lake Conestee, a former reservoir now filled with pollutant‐enriched sediments, located south of Greenville, South Carolina, USA, and other nearby reservoirs were collected analyzed for lead polycyclic aromatic hydrocarbons (PAHs). Hepatic ethoxyresorufin‐ O ‐deethylase (EROD), glutathione S ‐transferase (GST), UDP‐glucuronosyltransferase (UGT), sulfotransferase (SULT), erythrocyte δ‐aminolevulinic acid dehydratase (ALAD) measured in largemouth bass ( Micropterus...

During the Bremerhaven Workshop, sediment samples from 2 pollution gradients in North Sea were tested a 10 d static bioassay with infaunal amphipods.One gradient was downstream former drilling site, second offshore of Elbe-Weser plume German Bight.Four participating laboratories used basically same technique to test toxicity 1 3 different amphipod specles: Rhepoxynius abronius, Corophium volutator or Bathyporeia sarsi.The measured endpoints mortality and sublethal effects, including...

Despite carbon nanomaterials' (CNMs) potential to alter the bioavailability of adsorbed contaminants, information characterizing relationship between adsorption behavior and CNM-adsorbed contaminants is still limited. To investigate influence CNM morphology organic contaminant (OC) physicochemical properties on this relationship, isotherms were generated for a suite polycyclic aromatic hydrocarbons (PAHs) multiwalled nanotubes (MWCNTs) exfoliated graphene (GN) in conjunction with determining...