A5047682471- Plant Pathogens and Resistance
- Plant Disease Resistance and Genetics
- Potato Plant Research
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Genetic diversity and population structure
- Ecology and Conservation Studies
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
Obihiro University of Agriculture and Veterinary Medicine
2015-2024
Kobe University
2009-2018
Max Planck Institute for Plant Breeding Research
2015
Hokkaido Agricultural Research Center
2011-2013
SNPs in candidate genes Pain - 1, InvCD141 (invertases), SSIV (starch synthase), StCDF1 (transcription factor), LapN (leucine aminopeptidase), and cytoplasm type are associated with potato tuber yield, starch content and/or yield. Tuber yield (TY), (TSC), (TSY) complex characters of high importance for the crop general industrial production particular. DNA markers superior alleles that control natural variation TY, TSC, TSY could increase precision speed breeding new cultivars optimized...
Various wild species germplasm has been used in European potato breeding since the first introduction of (Solanum tuberosum L.) to Europe. As plant cytoplasmic genome including chloroplast and mitochondrial genomes is transmitted only through maternal parent, markers are useful tools programs determine types trace ancestors. The can be distinguished into six distinct (M, P, A, W, T, D). Male sterility was found genotypes with S. demissum-derived D-type cytoplasm stoloniferum-derived W/γ-type...
Abstract Cytoplasmic male sterility (CMS) is a phenotype wherein plants cannot develop normal organs because of mitochondrial genes. Several cytoplasmic factors are suggested to be involved in CMS phenotypes potatoes ( Solanum tuberosum ); however, reports on the relevant genes remain scarce. Many potato cultivars carry that cause CMS, resulting pollen sterility, thereby limiting their use as parents breeding. Therefore, identifying causal crucial for In this study, we focused T/β-type...
Abstract Potato and its wild relatives are distributed mainly in the Mexican highlands central Andes of South America. The American A-genome species, including cultivated potatoes, reproductively isolated from diploid species. Whole-genome sequencing has disclosed genome structure similarity, mostly potatoes their closely related In this study, we generated a chromosome-scale assembly Solanum bulbocastanum Dun., using PacBio long-read sequencing, optical mapping, Hi-C scaffolding...
The soil borne, obligate biotrophic fungus Synchytrium endobioticum causes tumor-like tissue proliferation (wart) in potato tubers and thereby considerable crop damage. Chemical control is not effective unfriendly to the environment. S. therefore a quarantined pathogen. emergence of new pathotypes aggravate this agricultural problem. best wart disease cultivation resistant varieties. Phenotypic screening for cultivars however time, labor material intensive. Breeding resistance would greatly...
A total of 476 accessions seven cultivated and 32 wild potato species previously characterized by nuclear DNA (nDNA) chloroplast (ctDNA) marker analyses were employed to the mitochondrial (mtDNA) analysis. Fourteen simple sequence repeat (SSR) markers with mononucleotide regions developed from mtDNA, although their variability was extremely low. Six mtDNA including three SSR disclosed 40 banding patterns that discriminated 63 different mtDNAs. For same set samples, 72 ctDNA 129 ctDNAs....
Abstract Background Tetrad sterility in potato is caused by a specific cytoplasm, called TSC sto , derived from the Mexican wild tetraploid species Solanum stoloniferum . Different S. accessions crossed as females with tuberosum resulted 12 fertile hybrids and 27 sterile exhibiting tetrad sterility. Results Whole-mitochondrial-genome sequencing was performed for two three Two to seven contigs, total assembly lengths ranging 462,716 535,375 bp, were assembled each hybrid. Unlike reference...
There are over 100 known species of cultivated potatoes and their wild relatives. Many these species, including potatoes, share the A genome; mainly distributed in South America reproductively isolated from Mexican diploid species. The only A-genome Mexico is Solanum verrucosum Schlechtendal, which also a maternal progenitor polyploid In this study, we constructed high-quality de novo assembly S. genome using PacBio long-read sequencing Hi-C scaffolding technologies. monohaploid clone (2n =...
A Mexican hexaploid wild potato species, Solanum demissum (dms), was only used as a female in previous breeding programs. The resulting clones with dms cytoplasm produced abundant, but non-functional pollen. 170 bp DNA fragment, named Band 1, originally detected the F1 hybrid between and S. tuberosum. In this study, sequenced region extended to 1,032 bp; nevertheless, it did not show any homology known sequences. This harboring 1 was, without introns, all transcribed mRNA maternally...
SUMMARY Mexican wild diploid potato species are reproductively isolated from A‐genome species, including cultivated potatoes; thus, their genomic relationships remain unknown. Solanum stoloniferum Schlechtd. et Bché. (2 n = 4 x 48, AABB) is a allotetraploid frequently used in breeding. We constructed chromosome‐scale assembly of the S. genome using PacBio long‐read sequencing and Hi‐C scaffolding technologies. The final consisted 1742 Mb, among which 745 Mb 713 were anchored to 12 B‐genome...
'Harimaru' is a new potato variety bred from cross between 'Saikai 35' as female parent and 'Pike' male parent. Marker selection was performed for 1,647 seedlings to combine resistance genes late blight (R1), Potato virus Y (Rychc), X (Rx1), golden cyst nematode (H1). In total, 194 selected clones were evaluated in the field, among which best clone officially released 'Harimaru'. Its yield slightly lower than local standard variety, 'May Queen'. However, it produces tasty potatoes, that do...
Abstract Newly developed potato varieties in Japan have never replaced existing old varieties, indicating genetic poverty our gene pool. To broaden the diversity, Andean primitive cultivated potatoes (Andigena) were used to create parental clones. A total of 184 Andigena accessions and 10 tetraploid Solanum tarijense -Andigena hybrid clones crossed with a breeding clone 10H17. From 6939 F 1 clones, 287 selected as long-day-adapted progeny evaluation using these pollen parents Atlantic, 95...