A5069375533- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Innovation and Socioeconomic Development
- Bacteriophages and microbial interactions
- Pacific and Southeast Asian Studies
- Genomics and Phylogenetic Studies
- Genetics, Aging, and Longevity in Model Organisms
- Pleistocene-Era Hominins and Archaeology
- Viral-associated cancers and disorders
- Cloud Computing and Resource Management
- Parvovirus B19 Infection Studies
- Infections and bacterial resistance
- Pneumonia and Respiratory Infections
- Vibrio bacteria research studies
- Australian Indigenous Culture and History
- Cytomegalovirus and herpesvirus research
- Microbial infections and disease research
- Mycobacterium research and diagnosis
University of Nevada, Reno
2020-2025
University of California, San Francisco
2019
Parks and Wildlife Service
1991
ABSTRACT Orthoflaviviruses are positive-sense single-stranded RNA viruses that hijack host proteins to promote their own replication. Zika virus (ZIKV) is infamous among orthoflaviviruses for its association with severe congenital birth defects, notably microcephaly. We previously mapped ZIKV-host protein interactions and identified the interaction between ZIKV non-structural 4A (NS4A) microcephaly ankyrin repeat LEM domain-containing 2 (ANKLE2). Using a fruit fly model, we showed NS4A...
Background/Objectives: Culex species mosquitoes are globally distributed and transmit several pathogens that impact animal public health, including West Nile virus, Usutu Plasmodium relictum. Despite their relevance, less widely studied than Aedes Anopheles mosquitoes. To expand the genetic tools used to study mosquitoes, we previously developed an optimized plasmid for transient Cas9 single-guide RNA (sgRNA) expression in quinquefasciatus cells generate gene knockouts. Here, established a...
Abstract Background Transfusion-related sepsis remains an important hospital infection control challenge. Investigation of septic transfusion events is often restricted by the limitations bacterial culture in terms time requirements and low yield setting prior antibiotic administration. Methods In 3 gram-negative cases, we performed metagenomic next-generation sequencing (mNGS) direct clinical blood specimens addition to standard culture-based approaches utilized for investigations. Pathogen...
Kaposi’s sarcoma-associated herpesvirus (KSHV) causes multiple malignancies in immunocompromised individuals. KSHV primarily establishes a lifelong latency infected humans during which only subset of viral genes is expressed while most the genome remains transcriptionally silent with condensed chromatin. However, lytic phase, undergoes dramatic changes chromatin landscape leading to active state expression and production progeny virions. Multiple cellular factors influence epigenetic gene...
ABSTRACT As an alternative and complementary approach to Cas9-based genome editing, Cas12a has not been widely used in mammalian cells largely due its strict requirement for the TTTV protospacer adjacent motif (PAM) sequence. Here, we report that Mb3Cas12a (Moraxella bovoculi AAX11_00205) can efficiently edit mouse based on TTV PAM sequence with minimal numbers of large on-target deletions or insertions. When sequence-targeting CRISPR (cr)RNAs 23 nt spacers are used, >70% founders...
The first step of any immune response is the recognition foreign molecular structures inside host organism. An important molecule that generally to eukaryotic cells long double-stranded RNA (dsRNA), which can be generated during virus replication. mechanisms sensing viral dsRNA are well-studied in mammalian systems but only poorly understood insects, including disease vectors such as Culex quinquefasciatus mosquitoes. These mosquitoes for arboviruses, West Nile virus, and species distributed...
Culex quinquefasciatus mosquitoes are a globally widespread vector of multiple human and animal pathogens, including West Nile virus, Saint Louis encephalitis lymphatic filariasis. Since the introduction virus to United States in 1999, cumulative 52,532 cases have been reported CDC, 25,849 (49.2%) neuroinvasive 2456 (5%) deaths. Viral infections elicit immune responses their mosquito vectors, RNA interference (RNAi) pathway considered be cornerstone antiviral response insects. To investigate...
A preliminary survey for Aboriginal sites in the Denison River valley was undertaken March 1989 by a team of eight people from Tasmanian Department Lands, Parks and Wildlife (now Parks, Heritage). Seven confirmed archaeological sites, all almost certainly providing evidence Pleistocene human occupation, were located.
Abstract Background Transfusion-related sepsis remains an important hospital infection control challenge. Investigating septic transfusion events is often restricted by the limitations of bacterial culture in terms time requirements and low yield setting prior antibiotic administration. Methods In three Gram-negative cases, we performed mNGS direct clinical blood specimens addition to standard culture-based approaches utilized for investigations. Pathogen detection leveraged IDSeq, a new...
Culture-confirmed bacteremia occurs in only a fraction of pneumonia cases. The degree to which microbial DNA circulates blood patients with pneumonia, however, is unknown. Detection cell-free from plasma promising new tool for infectious disease diagnosis. We conducted unbiased metagenomic next-generation sequencing (mNGS) paired and respiratory fluid 25 subjects, 18 (four concurrent bloodstream infections) seven non-infectious illnesses. Cell-free mNGS identified one or more...
Culture-confirmed bacteremia occurs in only a fraction of pneumonia cases. The degree to which microbial DNA circulates blood patients with pneumonia, however, is unknown. Detection cell-free from plasma promising new tool for infectious disease diagnosis. We conducted unbiased metagenomic next-generation sequencing (mNGS) paired and respiratory fluid 25 subjects, 18 (four concurrent bloodstream infections) seven non-infectious illnesses. Cell-free mNGS identified one or more...