A5085409826- Mathematical Biology Tumor Growth
- Glioma Diagnosis and Treatment
- Photoacoustic and Ultrasonic Imaging
- Cancer Cells and Metastasis
- Cell Image Analysis Techniques
- Optical Imaging and Spectroscopy Techniques
- Advanced Fluorescence Microscopy Techniques
- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Thermal Regulation in Medicine
- interferon and immune responses
- Metabolomics and Mass Spectrometry Studies
- Nanoplatforms for cancer theranostics
- Lanthanide and Transition Metal Complexes
- Medical Imaging Techniques and Applications
- Single-cell and spatial transcriptomics
- Analytical Chemistry and Chromatography
- MRI in cancer diagnosis
- Thermography and Photoacoustic Techniques
- Computational Drug Discovery Methods
- Cancer, Hypoxia, and Metabolism
- Cellular Mechanics and Interactions
- Retinoids in leukemia and cellular processes
- Tea Polyphenols and Effects
- Photodynamic Therapy Research Studies
University of Cambridge
2022-2025
Cancer Research UK
2023-2025
Foundation for Research and Technology Hellas
2014-2024
Cancer Research UK Cambridge Center
2022-2023
FORTH Institute of Electronic Structure and Laser
2021
University of Crete
2015-2019
Institute of Molecular Biology and Biotechnology
2018
University of Ioannina
2016
FORTH Institute of Computer Science
2015
Abstract Adjuvant Temozolomide is considered the front-line Glioblastoma chemotherapeutic treatment; yet not all patients respond. Latest trends in clinical trials usually refer to Doxorubicin; it can lead severe side-effects if administered high doses. While prognosis remains poor, little known about combination of two chemotherapeutics. Patient-derived spheroids were generated and treated with a range Temozolomide/Doxorubicin concentrations either as monotherapy or combination. Optical...
The application of accurate cancer predictive algorithms validated with experimental data is a field concerning both basic researchers and clinicians, especially regarding highly aggressive form cancer, such as Glioblastoma. In an aim to enhance prediction accuracy in realistic patient-specific environments, accounting for inter- intratumoral heterogeneity, we use patient-derived Glioblastoma cells from different patients. We focus on cell proliferation using vitro experiments estimate...
Abstract Multispectral optoacoustic tomography is a promising medical imaging modality that combines light and sound to provide molecular information at depths of several centimeters, based on the optical absorption endogenous chromophores, such as hemoglobin. Assessment inflammatory bowel disease has emerged clinical application tomography. In this context, preclinical studies in animal models are essential identify novel disease‐specific biomarkers understand findings from emerging pilot...
Radiotherapy is recognized globally as a mainstay of treatment in most solid tumors and essential both curative palliative settings. Ionizing radiation frequently combined with surgery, either preoperatively or postoperatively, systemic chemotherapy. Recent advances imaging have enabled precise targeting lesions yet substantial intratumoral heterogeneity means that planning monitoring remains clinical challenge therapy response can take weeks to manifest on conventional early indications...
Abstract Glioblastoma cells adopt migration strategies to invade into the brain parenchyma ranging from individual collective mechanisms, whose role and dynamics are not yet fully understood. In this work, we explore heterogeneity recapitulate its invasive patterns both in vitro , by utilizing primary along with U87MG cell line, silico adopting discrete, cell-based mathematics. cultured three-dimensionally an ECM-like substrate. The spheroids a novel cohesive pattern, mimicking perivascular...
Abstract Optoacoustic imaging (OAI) enables microscale of endogenous chromophores such as hemoglobin at significantly higher penetration depths compared to other optical technologies. Raster‐scanning optoacoustic mesoscopy (RSOM) has recently been shown identify superficial microvascular changes associated with human skin pathologies. In animal models, the depth afforded by RSOM can enable entirely new capabilities for noninvasive vascular structures in gastrointestinal tract, but exact...
Abstract Background/Objectives Pseudo-vascular network formation in vitro is considered a key characteristic of vasculogenic mimicry. While many cancer cell lines form pseudo-vascular networks, little known about the spatiotemporal dynamics these formations. Methods Here, we present framework for monitoring and characterising dynamic dissolution networks vitro. The combines time-resolved optical microscopy with open-source image analysis feature extraction statistical modelling. demonstrated...
Abstract The analysis of vascular morphology and functionality enables the assessment disease activity therapeutic effects in various pathologies. Raster‐scanning optoacoustic mesoscopy (RSOM) is an imaging modality that visualization superficial networks vivo. In murine models colitis, deep colon wall can be visualized by transrectal absorber guide raster‐scanning (TAG‐RSOM). order to accelerate implementation this technology translational studies inflammatory bowel disease,...
Glioblastoma multiforme is the most aggressive type of glioma and common malignant primary intra-axial brain tumor. In an effort to predict evolution disease optimize therapeutical decisions, several models have been proposed for simulating growth pattern glioma. One latest incorporates cell proliferation invasion, angiogenic net rates, oxygen consumption, vasculature. These factors, particularly oxygenation levels, are considered fundamental factors tumor heterogeneity compartmentalization....
NMR based metabolomics clarify the<italic>Origanum dictamnus</italic>tea effect upon the human urine metabolome.
Glioblastoma is the most malignant brain tumor among adults. Despite multimodality treatment, it remains incurable, mainly because of its extensive heterogeneity and infiltration in parenchyma. Recent evidence indicates dysregulation expression Promyelocytic Leukemia Protein (PML) primary samples. PML implicated various ways cancer biology. In brain, participates physiological migration neural progenitor cells, which have been hypothesized to serve as cell origin Glioblastoma. The role...
Abstract Objective: The formation of functional vasculature in solid tumours enables delivery oxygen and nutrients, is vital for effective treatment with chemotherapeutic agents. Longitudinal characterisation vascular networks can be enabled using mesoscopic photoacoustic imaging, but requires accurate image co-registration to precisely assess local changes across disease development or response therapy. Co-registration imaging challenging due the complex nature generated signal, including...
Breast cancer and Glioblastoma brain are aggressive malignancies with poor prognosis. In this study primary secondary breast spheroids formed treated the well-known Temozolomide Doxorubicin chemotherapeutics, respectively. A custom multi-angle Light Sheet Fluorescence Microscope is employed for high resolution imaging of both cell spheroids. Such a technique successful in realizing pre-clinical drug screening, while enables discrimination among physiologic tumor parameters. LSFM technique,...
Brain tumor lesions (BTL), i.e. high grade gliomas, are known to have a prominence of vasculature, which is promoted through hypoxia mechanisms and differential expression vascular endothelial growth factor (VEGF). Imaging techniques such as dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) with intra-venous (i.v.) administration Gd-based agent (GBCA) successfully used for the diagnosis characterization BTLs. Tracer kinetics plays an important role in DCE-MRI by assessing vessel...
Breast cancer and Glioblastoma brain are severe malignancies with poor prognosis. In this study, primary secondary breast spheroids formed treated the well-known Temozolomide Doxorubicin chemotherapeutics, respectively. High resolution imaging of both cell is possible using a custom multi-angle Light Sheet Fluorescence Microscope. Such technique successful in realizing preclinical drug screening, while enables discrimination among physiologic tumor parameters.
Abstract Background: Adjuvant Temozolomide is considered the front-line Glioblastoma chemotherapeutic treatment; yet not all patients respond. Latest trends in clinical trials usually refer to Doxorubicin; it can lead severe side-effects if administered high doses. While prognosis remains poor, little known about combination of two chemotherapeutics. Methods: Patient-derived spheroids were generated and treated with a range Temozolomide/Doxorubicin concentrations either as monotherapy or...
Abstract Pseudo-vascular network formation capacity in vitro is considered a key characteristic of vasculogenic mimicry. While many cancer cell lines are known to form pseudo-vascular networks, little about the spatiotemporal dynamics these formations. Here, we present framework for monitoring and characterising dynamic dissolution networks . The combines time-resolved optical microscopy with open-source image analysis feature extraction statistical modelling. demonstrated by comparing...