A5000981525- Advancements in Transdermal Drug Delivery
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Immunotherapy and Biomarkers
- Asthma and respiratory diseases
- CAR-T cell therapy research
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Ubiquitin and proteasome pathways
- Advanced Biosensing Techniques and Applications
- Dermatology and Skin Diseases
- Dietary Effects on Health
- Parasitic Diseases Research and Treatment
- Microfluidic and Bio-sensing Technologies
- Ocular Surface and Contact Lens
- Allergic Rhinitis and Sensitization
- Microfluidic and Capillary Electrophoresis Applications
- T-cell and B-cell Immunology
Massachusetts Institute of Technology
2019-2021
University of Wisconsin–Madison
2013-2019
Allen Institute
2018
Koch Institute for Integrative Cancer Research At MIT
2018
Minimally invasive technologies that can sample and detect cell-free nucleic acid biomarkers from liquid biopsies have recently emerged as clinically useful for early diagnosis of a broad range pathologies, including cancer. Although blood has so far been the most commonly interrogated bodily fluid, skin interstitial fluid mostly overlooked despite containing same variety molecular originating cells surrounding capillaries. Emerging to this in pain-free minimally-invasive manner often take...
The clinical application of cytokine therapies for cancer treatment remains limited due to severe adverse reactions and insufficient therapeutic effects. Although localization by intratumoral administration could address both issues, the rapid escape soluble cytokines from tumor invariably subverts this effort. We find that a fused collagen-binding protein lumican prolongs local retention markedly reduces systemic exposure. Combining lumican-cytokine fusions with immunotherapies...
Significance Vaccine kinetics, the timing of antigen and/or adjuvant delivery to lymphoid organs, have recently been shown substantially influence immune responses subunit vaccines, but strategies control vaccine kinetics in a clinically translatable manner are still lacking. MNs arrays solid micron-sized projections that utilized early clinical trials for delivery. Here, we investigated whether implantable MN skin patches could be used kinetics. We show sustained intradermal release an HIV...
A minimally invasive microneedle device allows repeated sampling of tissue-resident immune cell populations from the skin.
Efficacious vaccines require antigens that elicit productive immune system activation. Antigens afford robust antibody production activate both B and T cells. Elucidating the antigen properties enhance B-T cell communication is difficult with traditional antigens. We therefore used ring-opening metathesis polymerization to access chemically defined, multivalent containing epitopes explore how structure impacts activation communication. The bifunctional were designed so backbone substitution...
Abstract We show that treatment with the FDA-approved anti-parasitic drug ivermectin induces immunogenic cancer cell death (ICD) and robust T infiltration into breast tumors. As an allosteric modulator of ATP/P2X4/P2X7 axis which operates in both immune cells, also selectively targets immunosuppressive populations including myeloid cells Tregs, resulting enhanced Teff/Tregs ratio. While neither agent alone showed efficacy vivo, combination therapy checkpoint inhibitor anti-PD1 antibody...
B cells detect foreign antigens through their cell antigen receptor (BCR). The BCR, when engaged by antigen, initiates a signaling cascade. Concurrent with is endocytosis of the BCR complex, which acts to downregulate and facilitate uptake for processing display on surface. relationship between poorly defined. Here, we explore interplay that either promote or inhibit activation. Specifically, synthetic were generated engage alone both inhibitory co-receptor CD22. lectin CD22, member Siglec...
Subunit vaccines can have excellent safety profiles, but their ability to give rise robust immune responses is often compromised. For glycan-based vaccines, insufficient understanding of B and T cell epitope combinations that yield optimal activation hinders optimization. To determine which antigen features promote desired IgG responses, we synthesized epitope-functionalized polymers using ring-opening metathesis polymerization (ROMP) assessed the effect loading. The most were induced by...
Abstract We show that treatment with the FDA-approved anti-parasitic drug ivermectin induces immunogenic cancer cell death (ICD) and robust T infiltration into breast tumors. As an allosteric modulator of ATP/P2×4/P2×7 axis which operates in both immune cells, also selectively targets immunosuppressive populations including myeloid cells Tregs, resulting enhanced Teff/Tregs ratio. While neither agent alone showed efficacy vivo , combination therapy checkpoint inhibitor anti-PD1 antibody...