A5041494111- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Plant Virus Research Studies
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Studies on Chitinases and Chitosanases
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- RNA modifications and cancer
- Diffusion and Search Dynamics
University of Missouri–St. Louis
2015-2017
University of North Carolina at Chapel Hill
2009
University of Wisconsin–Madison
2006
Movement of transposons causes insertions, deletions, and chromosomal rearrangements potentially leading to premature lethality in Drosophila melanogaster. To repress these elements combat genomic instability, eukaryotes have evolved several small RNA-mediated defense mechanisms. Specifically, somatic cells, endogenous interfering (esi)RNAs suppress retrotransposon mobility. EsiRNAs are produced by Dicer-2 processing double-stranded RNA precursors, yet the origins precursors unknown. We show...
While non-specific DNA plays a role in target localization for many recombinases, transcription factors and restriction enzymes, the importance of interactions transposases has not been investigated. Here, we discuss DNA-Tn 5 Transposase (Tnp) suggest how they stabilize Tnp modulate 19 bp recognition end sequences (ESes). protection assays indicate that full-length interacts efficiently with supercoiled does contain ESes. These significantly prolong lifetime Tnp, vitro . The balance between...
A core cleavage complex (CCC) consisting of CPSF73, CPSF100, and Symplekin is required for cotranscriptional 3' end processing all metazoan pre-mRNAs, yet little known about the in vivo molecular interactions within this complex. The CCC a component two distinct complexes, cleavage/polyadenylation that processes nonpolyadenylated histone pre-mRNAs. RNAi-depletion factors Drosophila culture cells causes reduction activity on mRNAs, resulting read through transcription. In contrast, only mRNA...
X-ray cocrystal structures of Tn5 transposase (Tnp) bound to its 19 base pair (bp) recognition end sequence (ES) reveal contacts between a β-loop (amino acids 240−260) and positions 3, 4, 5, 6 the ES. Here, we show that mutations residues in this loop affect both vivo vitro transposition. Most are detrimental, whereas some at position 242 cause hyperactivity. More specifically, every individual step transposition tested. Mutants performing less efficiently also form fewer synaptic complexes,...
Understanding regulation of transposon movement in somatic cells is important as mobile elements can cause detrimental genomic rearrangements. Generally, transposons move via one 2 mechanisms; retrotransposons utilize an RNA intermediate, therefore copying themselves and amplifying throughout the genome, while terminal inverted repeat (TIR Tns) excise DNA sequences from genome integrate into a new location. Our recently published work indicates that Drosophila tissue culture are actively...
Endogenous small interfering (esi)RNAs repress mRNA levels and retrotransposon mobility in Drosophila somatic cells by poorly understood mechanisms. 21 nucleotide esiRNAs are primarily generated from retrotransposons two inverted repeat (hairpin) loci culture a Dicer2 dependent manner. Additionally, proteins involved 3' end processing, such as Symplekin, CPSF73 CPSR100, have been recently implicated the esiRNA pathway.Here we present evidence of overlap between essential RNA metabolic...
Metazoan histone pre‐mRNAs end in a stem‐loop (SL) structure rather than poly(A) tail. Formation of mRNAs requires cleavage directed by the SL, which binds SLBP, and downstream element, is recognized U7 snRNP. In D. melanogaster , signals located normal pre‐mRNA sites are utilized when inhibited. Knockdown CPSF73, CPSF100, Symplekin results polyadenylation mRNA, while depletion CstF50, 64 77, CPSF 30 160 does not affect processing, suggesting that CPSF100 comprise core common factor....