Piezo1 mechanosensitive ion channel plays a important role in vascular physiology and disease. This study aimed to elucidate the altered signaling elicited by activation arteries of type 2 diabetes. Ten- 12-week-old male C57BL/6 (control) diabetic mice (db

Phellinus linteus is a well-known medicinal mushroom that widely used in Asian countries. In several experimental models, extracts were reported to have various biological effects, including anti-inflammatory, anti-cancer, hepatoprotective, anti-diabetic, neuroprotective, and anti-angiogenic activity. the present study, bioactive compounds, palmitic acid ethyl ester linoleic acid, identified linteus. The intermediate-conductance calcium-activated potassium channel (IKCa) plays an important...

Autophagy is an intracellular degradation system that disassembles cytoplasmic components through autophagosomes fused with lysosomes. Recently, it has been reported autophagy associated cardiovascular diseases, including pulmonary hypertension, atherosclerosis, and myocardial ischemia. However, the involvement of in hypertension not well understood. In present study, we hypothesized excessive contributes to dysfunction mesenteric arteries angiotensin II (Ang II)-induced hypertensive mice....

Background An orally active synthetic adiponectin receptor agonist, AdipoRon has been suggested to ameliorate insulin resistance, and glucose tolerance. However, the chronic effect of in vascular dysfunction type 2 diabetes not studied yet. Thus, this study, we examined whether improves function diabetes. Methods Type diabetic (db-/db-) mice were treated with (10 mg/kg/everyday, by oral gavage) for weeks. Body weight blood levels recorded every other day during experimental period. Diameter...

Dapagliflozin, a sodium–glucose co-transporter 2 (SGLT2) inhibitor, is an antidiabetic medication that reduces blood glucose. Although it well known dapagliflozin has additional benefits beyond glycemic control, such as reducing pressure and lowering the risk of cardiovascular events, no sufficient research data are available on direct effect function. Thus, in this study, we investigated vascular isolated rat coronary arteries. The left descending arteries 13-week-old male Sprague Dawley...

Vanillin is a phenolic aldehyde, which found in plant species of the Vanilla genus. Although recent studies have suggested that vanillin has various beneficial properties, effect on blood vessels not been studied well. In present study, we investigated whether vascular effects rat mesenteric resistance arteries. To examine vanillin, measured isometric tension arteries using multi-wire myograph system. After were pre-contracted with high K+ (70 mM) or phenylephrine (5 µM), was administered....

Aim: Piezo1, a mechanosensitive ion channel, plays significant role in vascular physiology and disease. This study aimed to elucidate the altered signaling elicited by Piezo1 activation arteries of type 2 diabetes. Methods: Ten-to 12-week-old male C57BL/6 (control) diabetic mice (db − /db ) were used. The second-order mesenteric (~150 mm) used for isometric tension experiments. Western blot analysis immunofluorescence staining performed observe protein expression. Results: was significantly...

Trachelospermi caulis (T. caulis) has been used as a traditional herbal medicine in Asian countries. Although it is well known that T. beneficial effects, no sufficient research data are available on the cardiovascular effect of caulis. We investigated whether extract vascular effects rat resistance arteries this study.To examine affects reactivity, we measured isometric tension mesenteric using multi-wire myograph system. was administered after were pre-contracted with high K+ (70 mM) or...

Electrophoretic mobility shift assay (EMSA) technology has been widely employed for the analysis of transcription factors such as Forkhead box protein M1 (FOXM1). However, application high-throughput screening (HTS) in performing, analyses are limited it uses time consuming electrophoresis procedure and radioisotopes. In this study, we developed a FOXM1-DNA binding domain (DBD) based on time-resolved fluorescence energy transfer (TR-FRET) that enables HTS inhibitors interaction. This was...

Alpinia officinarum (A. officinarum) is known to exhibit a beneficial effect for anti-inflammatory, anti-oxidant, and anti-hyperlipidemic effects. However, no sufficient research data are available on the cardiovascular of A. officinarum. Thus, in this study, we investigate whether extract has direct effects vascular reactivity.To examine affects functionality, measured isometric tension rat mesenteric resistance arteries using wire myograph. After were pre-contracted with high-K+ (70 mM),...

Ezetimibe is a lipid-lowering agent that selectively inhibits cholesterol absorption by binding to the Niemann-Pick C1-like 1 (NPC1L1) protein. Although it well known administration of ezetimibe in hypercholesterolemia patients reduces risk cardiovascular events through attenuation atherosclerosis, studies on direct effect vascular function are not sufficient. The aim present study was investigate effects rat mesenteric arteries. In study, 12-week-old male Sprague Dawley rats were used....