A5069816964- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Trypanosoma species research and implications
- RNA regulation and disease
- Biochemical and Molecular Research
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Research on Leishmaniasis Studies
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Cardiomyopathy and Myosin Studies
- HIV/AIDS drug development and treatment
- Parasitic Infections and Diagnostics
- Cancer-related gene regulation
- Metalloenzymes and iron-sulfur proteins
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Metabolism and Genetic Disorders
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Phytoplasmas and Hemiptera pathogens
- Enzyme Structure and Function
- Muscle Physiology and Disorders
- Cytomegalovirus and herpesvirus research
John Brown University
2024
The Ohio State University
2014-2023
Brown University
2023
Taylor's University
2018
Tufts University
2018
Jean Mayer Human Nutrition Research Center on Aging
2018
University of Miami
2018
University of Cincinnati
2015
Columbus Oncology and Hematology Associates
2014
University of California, Los Angeles
1997-2008
The combination of Reverse Transcription (RT) and high-throughput sequencing has emerged as a powerful to detect modified nucleotides in RNA via analysis either abortive RT-products or the incorporation mismatched dNTPs into cDNA. Here we simultaneously analyze both parameters detail with respect occurrence N-1-methyladenosine (m1A) template RNA. This naturally occurring modification is associated structural effects, but it also known mediator antibiotic resistance ribosomal In probing...
Mitochondrial genomes generally encode a minimal set of tRNAs necessary for protein synthesis. However, number eukaryotes import from the cytoplasm into their mitochondria. For instance, Saccharomyces cerevisiae imports cytoplasmic tRNA Gln mitochondrion without any added factors. Here, we examine existence similar active system in mammalian We have used subcellular RNA fractions rat liver and human cells to perform RT-PCR with oligonucleotide primers specific nucleus-encoded CUG UUG...
Aminoacyl-tRNA (aa-tRNA) formation, an essential process in protein biosynthesis, is generally achieved by direct attachment of amino acid to tRNA the aa-tRNA synthetases. An exception Gln-tRNA synthesis, which eukaryotes catalyzed glutaminyl-tRNA synthetase (GlnRS), while most bacteria, archaea, and chloroplasts employ transamidation pathway, a tRNA-dependent glutamate modification generates Gln-tRNA. Mitochondrial synthesis carried out normally mitochondrial enzymes organelle-encoded tRNAs...
Accurate selection of amino acids is essential for faithful translation the genetic code. Errors during acid are usually corrected by editing activity aminoacyl-tRNA synthetases such as phenylalanyl-tRNA (PheRS), which edit misactivated tyrosine. Comparison cytosolic and mitochondrial PheRS from yeast Saccharomyces cerevisiae suggested that organellar protein might lack activity. Yeast was found to contain an site, upon disruption abolished both cis trans Tyr-tRNA(Phe). Wild-type lacked...
Adenosine-to-inosine editing in the anticodon of tRNAs is essential for viability. Enzymes mediating tRNA adenosine deamination bacteria and yeast contain cytidine deaminase-conserved motifs, suggesting an evolutionary link between two reactions. In trypanosomatids, undergo both cytidine-to-uridine adenosine-to-inosine editing, but relationship reactions unclear. Here we show that down-regulation Trypanosoma brucei tRNA-editing enzyme by RNAi leads to a reduction C-to-U A-to-I vivo....
Ribosomal ribonucleic acid (RNA), transfer RNA and other biological or synthetic polymers can contain nucleotides that have been modified by the addition of chemical groups. Traditional Sanger sequencing methods cannot establish nature sequence these modified-nucleotide containing oligomers. Mass spectrometry (MS) has become conventional approach for determining nucleotide composition, modification status RNAs. Modified RNAs are analyzed MS using collision-induced dissociation tandem mass...
Transfer RNA modifications play pivotal roles in protein synthesis. N6-threonylcarbamoyladenosine (t6A) and its derivatives are found at position 37, 3΄-adjacent to the anticodon, tRNAs responsible for ANN codons. These universally conserved all domains of life. t6A have pleiotropic functions synthesis including aminoacylation, decoding translocation. We previously discovered a cyclic form (ct6A) as chemically labile derivative from bacteria, fungi, plants protists. Here, we report...
Abstract Every type of nucleic acid in cells undergoes programmed chemical post-transcriptional modification. Generally, modification enzymes use substrates derived from intracellular metabolism, one exception is queuine (q)/queuosine (Q), which eukaryotes obtain their environment; made by bacteria and ultimately taken into eukaryotic via currently unknown transport systems. Here, we a combination molecular, cell biology biophysical approaches to show that Trypanosoma brucei tRNA Q levels...
Abstract Transfer RNAs (tRNAs) are key players in protein synthesis. To be fully active, tRNAs undergo extensive post-transcriptional modifications, including queuosine (Q), a hypermodified 7-deaza-guanosine present the anticodon of several bacteria and eukarya. Here, molecular biochemical approaches revealed that protozoan parasite Trypanosoma brucei, Q-containing have preference for U-ending codons asparagine, aspartate, tyrosine histidine, analogous to what has been described other...
The essential deamination of adenosine A
ABSTRACT Successful pathogens must be able to adapt a multitude of stressors imposed by their host. Acinetobacter baumannii has emerged as major global health threat due its exceptional ability hostile environments and skyrocketing rates multidrug resistance. Recent studies have begun explore the importance tRNA methylation in regulation bacterial stress responses, including adaptation antibiotic oxidative stresses. However, methyltransferases (trms) not been investigated A. . Bioinformatic...
Editing of tRNAs is widespread in nature and either changes the decoding properties or restores folding a tRNA. Unlike phylogenetically disperse adenosine (A) to inosine (I) editing, cytosine (C) uridine (U) editing has only been previously described organellar tRNAs. We have shown that cytoplasmic tRNA(Thr)(AGU) undergoes two distinct events anticodon loop: C U A I. In vivo, every inosine-containing tRNA(Thr) also edited at position 32. vitro, stimulates conversion I wobble base. Although...