A5042905704- CRISPR and Genetic Engineering
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Pluripotent Stem Cells Research
- Plant Virus Research Studies
- Invertebrate Immune Response Mechanisms
- CAR-T cell therapy research
- Physiological and biochemical adaptations
- Autophagy in Disease and Therapy
- Neurobiology and Insect Physiology Research
- RNA Interference and Gene Delivery
- Innovation and Socioeconomic Development
- Retinal Development and Disorders
- Microtubule and mitosis dynamics
University of Glasgow
2013-2024
Insect osmoregulation is subject to highly sophisticated endocrine control. In Drosophila, both Drosophila kinin and tyramine act on the Malpighian (renal) tubule stellate cell activate chloride shunt conductance, so increase fluid production rate. known through intracellular calcium, but mode of action not known. Here, we used a transgenically encoded GFP::apoaequorin translational fusion, targeted either principal or cells under GAL4/UAS control, demonstrate that indeed acts raise calcium...
Abstract Background The field of genome editing has been revolutionized by the development an easily programmable tool, CRISPR–Cas9. Despite its promise, off-target activity Cas9 posed a great disadvantage for purposes causing DNA double strand breaks at locations and unwanted outcomes. Furthermore, gene integration applications, which introduce transgene sequences, transgenes to sites could be harmful, hard detect, reduce faithful efficiency. Method Here we report multicolour fluorescence...
Chromosomal rearrangements of the mixed lineage leukaemia (MLL, also known as KMT2A) gene on chromosome 11q23 are amongst most common genetic abnormalities observed in human acute leukaemias. MLL (MLLr) cytogenetic infant and childhood myeloid (AML) lymphocytic (ALL) do not normally acquire secondary mutations compared to other To model these leukaemias, we have used clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 editing induce MLL-AF9 (MA9) chromosomal murine...
Blood disorders like B-thalassemia or sickle cell anaemia obligate patients to require constant blood transfusions. These, among others, make a highly demanded resource in the medical field. has been obtained from in-vitro erythroid differentiation embryonic stem cells induced pluripotent cells, but this tends produce haemoglobin. In project, we aim use CRISPR/Cas9 modified version, combined with an aptamer approach, alter expression of β-globin locus upregulating gamma globin gene foetal...