A5031749819- CRISPR and Genetic Engineering
- Retinal Development and Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- CAR-T cell therapy research
- Pluripotent Stem Cells Research
University of Glasgow
2024
Abstract Leukaemias, driven by mutations in hematopoietic stem cells (HSCs), rely on interactions with the bone marrow (BM) niche and other cell populations such as mesenchymal stromal (MSCs) for growth survival. While chimeric antigen receptor (CAR) T-cell therapy shows promise hematological malignancies, its application to acute myeloid leukaemia (AML) is hindered tumour heterogeneity off-target toxicity. Combining CRISPR-Cas9 gene editing CAR has potential selectively targeting AML while...
Abstract Background The field of genome editing has been revolutionized by the development an easily programmable tool, CRISPR–Cas9. Despite its promise, off-target activity Cas9 posed a great disadvantage for purposes causing DNA double strand breaks at locations and unwanted outcomes. Furthermore, gene integration applications, which introduce transgene sequences, transgenes to sites could be harmful, hard detect, reduce faithful efficiency. Method Here we report multicolour fluorescence...