A5087049435- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Veterinary Equine Medical Research
- Reproductive biology and impacts on aquatic species
- Ovarian function and disorders
- Reproductive Health and Technologies
- RNA Interference and Gene Delivery
- Veterinary Medicine and Surgery
- Pelvic floor disorders treatments
- Effects of Environmental Stressors on Livestock
- Reproductive System and Pregnancy
- Educational Technology in Learning
- Organoselenium and organotellurium chemistry
- Phytoestrogen effects and research
- Telemedicine and Telehealth Implementation
- Reproductive tract infections research
- Selenium in Biological Systems
- Metabolism and Genetic Disorders
- Animal Genetics and Reproduction
- Aldose Reductase and Taurine
- Redox biology and oxidative stress
- Gut microbiota and health
- Tea Polyphenols and Effects
- Mitochondrial Function and Pathology
Universidad Cardenal Herrera CEU
2019-2024
Universidad de Extremadura
2012-2017
Newmarket Equine Hospital
2013
Mitochondria have been proposed as the major source of reactive oxygen species in somatic cells and human spermatozoa. However, no data regarding role mitochondrial ROS production stallion spermatozoa are available. To shed light on electron transport chain origin oxidative stress spermatozoa, specific inhibitors complex I (rotenone) III (antimycin-A) were used. Ejaculates from seven Andalusian stallions collected incubated BWW media at 37 °C presence rotenone, antimycin-A or control...
To investigate the hypothesis that oxidative phosphorylation is a major source of ATP to fuel stallion sperm motility, was suppressed using mitochondrial uncouplers CCCP and 2,4,-dinitrophenol (DNP) by inhibiting respiration at complex IV sodium cyanide or level synthase oligomycin-A. As dysfunction may also lead stress, production reactive oxygen species monitored simultaneously. All inhibitors reduced content, but oligomycin-A did so most profoundly. Oligomycin-A significantly membrane...
Apoptosis has been recognized as a cause of sperm death during cryopreservation and infertility in humans, however there is no data on its role conservation refrigeration; autophagy not described to date mature sperm. We investigated the apoptosis cooled storage stallion spermatozoa. Samples from seven stallions were split; half ejaculate was processed by single layer centrifugation, while other extended unprocessed, stored at 5°C for five days. During time storage, motility (CASA, daily)...
AKT, also referred to as protein kinase B (PKB or RAC), plays a critical role in controlling cell survival and apoptosis. To gain insights into the mechanisms regulating sperm after ejaculation, of AKT was investigated stallion spermatozoa using specific inhibitor phosphoflow approach. Stallion were washed incubated Biggers-Whitten-Whittingham medium, supplemented with 1% polyvinyl alcohol (PVA) presence 0 (vehicle), 10, 20 30 μM SH5, an inhibitor. SH5 treatment reduced percentage displaying...
PGF
Oxidative stress has been linked to sperm death and the accelerated senescence of cryopreserved spermatozoa. However, molecular mechanisms behind this phenomenon remain poorly understood. Reactive oxygen species (ROS) are considered relevant signaling molecules for function, only becoming detrimental when ROS homeostasis is lost. We hereby hypothesize that a major component alteration in spermatozoa exhaustion intrinsic antioxidant defense mechanisms. To test hypothesis, semen from seven...
Lactic acid bacteria (LAB) dominate human vaginal microbiota and inhibit pathogen proliferation. In other mammals, LAB do not microbiota, however shifts of dominant microorganisms occur during ovarian cycle. The study objectives were to characterize equine in mares by culture-dependent independent methods describe its variation estrus diestrus. Vaginal swabs from 8 healthy adult Arabian obtained For processing, isolated on Columbia blood agar (BA) Man Rogosa Sharpe (MRS) agar. comprised only...
Stallion spermatozoa are highly dependent on oxidative phosphorylation for ATP production to achieve normal sperm function and fuel the motility. The aim of this study was evaluate response equine under capacitating conditions inhibition mitochondrial complex I by rotenone test whether epigallocatechin-3-gallate (EGCG), a natural polyphenol component green tea, could counteract effect. After 2-h incubation stallion in modified Tyrode's medium, (100 nm, 500 nm 5 μm) EGCG (10, 20 60 μm), alone...
Contents Flow cytometry is considered the only reliable method for separation of X and Y chromosome bearing spermatozoa in equines. The MoFlo SX DP sorter highly efficient, allowing production foals desired sex. However, to achieve acceptable pregnancy rates currently used protocol requires working with fresh semen obtained close to, or at, sorting facility. An alternative was tested during two consecutive breeding seasons. Fresh stallion cooled 20 h, which staining Hoechst 33342 took place....
Abstract Ebselen (2‐phenyl‐1,2‐benzisoselenazol‐3(2H)‐one) is an organoselenium radical scavenger compound, which has strong antioxidant and anti‐inflammatory effects. However, evidence suggests that this compound could exert deleterious actions on cell physiology. In study, we have analyzed the effect of ebselen rat pancreatic AR42J cells. Cytosolic free‐Ca 2+ concentration ([Ca ] c ), cellular oxidative status, setting endoplasmic reticulum stress, phosphorylation major mitogen‐activated...
Contents To investigate the mechanisms inducing sperm death after ejaculation, stallion ejaculates were incubated in BWW media during 6 h at 37°C. At beginning of incubation period and 1, 2, 4 motility kinematics ( CASA ), mitochondrial membrane potential permeability integrity evaluated (flow cytometry). Also, same time intervals, active caspase 3, hydrogen peroxide, superoxide anion cytometry) Akt phosphorylation evaluated. Major decreases function occurred incubation, although 1 decrease...
The effect of embryo reinsertion immediately after flushing was studied to determine its on luteal function, pregnancy outcome, endometrial bacterial contamination, and morphology. In Experiment 1, eight mares were used during 32 cycles (8 in each group): for the first two groups, inseminated flushed 8 days ovulation prostaglandin F2α not administered: group EF-ET (embryo transfer) reinserted same donor mare, while EF group, no further procedure performed. third (ET), non-inseminated...
The effect of embryo reinsertion immediately after flushing was studied. In Experiment 1, eight mares were used during 32 cycles (8 in each group). For the first two groups, inseminated flushed 8 days ovulation and prostaglandin F2α not administered: group EF-ET (embryo transfer) reinserted same donor mare, while EF group, no further procedure performed. third (ET), non-inseminated (recipients) received a Day embryo. Progesterone concentration measured before EF/ET 72 h three groups. 2,...