A5001912536- interferon and immune responses
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- HIV Research and Treatment
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Phagocytosis and Immune Regulation
- Herpesvirus Infections and Treatments
- Erythrocyte Function and Pathophysiology
- Blood transfusion and management
- Blood groups and transfusion
- Pancreatic function and diabetes
- Urological Disorders and Treatments
- Pluripotent Stem Cells Research
- Liver physiology and pathology
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Neonatal Respiratory Health Research
- HIV/AIDS Research and Interventions
- Reproductive System and Pregnancy
National University of San Marcos
2025
Vitalant
2020-2023
Pacific Research Institute
2020-2021
Broad Center
2017
Reproductive Science Center
2017
University of California, San Francisco
2017
Abstract The DNA exonuclease three-prime repair 1 (TREX1) is critical for preventing autoimmunity in mice and humans by degrading endogenous cytosolic DNA, which otherwise triggers activation of the innate cGAS/STING pathway leading to production type I IFNs. As tumor cells are prone aberrant accumulation, we hypothesized that they critically dependent on TREX1 activity limit their immunogenicity. Here, show cells, restricts spontaneous pathway, subsequent induction a IFN response. result,...
Cryopreservation is known to destabilise spermatozoa and associated with deficiencies in protamine levels increased DNA fragmentation, which can reduce fertility various species. The objective of this study was evaluate the impact cryopreservation on fragmentation alpaca spermatozoa. A total 108 testicles/epididymides were collected from a slaughterhouse sperm recovered cauda epididymis. Only samples meeting criteria > 10 g weight, 3 cm length, 30% motility, 50 million spermatozoa/mL...
We examined the contribution of fetal membranes, amnion and chorion, to human embryonic hematopoiesis. A population cells displaying a hematopoietic progenitor phenotype (CD34++ CD45low) origin was present in chorion at all gestational ages, associated with stromal or near blood vessels, but absent amnion. Prior 15 weeks gestation, these lacked vivo engraftment potential. Differences chemokine receptor β1 integrin expression profiles progenitors between first second trimesters suggest that...
Animal models are vital to the study of transfusion and development new blood products. Post-transfusion recovery human components can be studied in mice, however, there is a need identify strains that best tolerate xenogeneic transfusions, as well optimize such protocols. Specifically, importance using immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) has been questioned. In this study, wild-type NSG mice were compared hosts for transfusions with outcomes quantified by flow cytometric...
<div>Abstract<p>The DNA exonuclease three-prime repair 1 (TREX1) is critical for preventing autoimmunity in mice and humans by degrading endogenous cytosolic DNA, which otherwise triggers activation of the innate cGAS/STING pathway leading to production type I IFNs. As tumor cells are prone aberrant accumulation, we hypothesized that they critically dependent on TREX1 activity limit their immunogenicity. Here, show cells, restricts spontaneous pathway, subsequent induction a IFN...
Abstract The cryopreservation of hematopoietic cells using dimethyl sulfoxide (DMSO) and serum is a common procedure used in transplantation. However, DMSO has clinical biological side effects due to its toxicity, introduces variation safety risks. Inspired by natural antifreeze proteins, novel class ice-interactive cryoprotectants was developed. corresponding DMSO-, protein-, serum-free media candidates were screened through series assays human cell lines, peripheral blood cells, bone...
Background &amp; Aims Hepatocytic cells found during prenatal development have unique features compared to their adult counterparts, and are believed be the precursors of pediatric hepatoblastoma. The cell-surface phenotype hepatoblasts hepatoblastoma cell lines was evaluated discover new markers these gain insight into hepatocytic phenotypes origins Methods Human midgestation livers four were screened using flow cytometry. Expression over 300 antigens on defined by expression CD326...
<p>Supplementary Figure 1. TREX1 limits an ISG response in CT26 and STING-expressing MC38 cells.</p>
<p>Supplementary Figure 5. TREX1 loss remodels an immunosuppressive myeloid tumor microenvironment</p>
<p>Supplementary Figure 4. Single-cell RNA sequencing workflow and ISG expression across immune cells in the tumor microenvironment</p>
<p>Supplementary Figure 2. Tumor-intrinsic TREX1 loss impairs CT26 tumor growth in immunocompetent mice a cGAS dependent manner.</p>
<p>Supplementary Figure 1. TREX1 limits an ISG response in CT26 and STING-expressing MC38 cells.</p>
<p>Supplementary Figure 6. TREX1 loss increases CD8 T cell and NK activation, limits exhaustion enhances the potency of immune checkpoint blockade therapy</p>
<p>Supplementary Figure 3. Assessment of TREX1’s catalytic activity and cGAS dependency.</p>
<p>Supplementary Figure 5. TREX1 loss remodels an immunosuppressive myeloid tumor microenvironment</p>
<p>Supplementary Figure 6. TREX1 loss increases CD8 T cell and NK activation, limits exhaustion enhances the potency of immune checkpoint blockade therapy</p>
<p>Supplementary Figure 2. Tumor-intrinsic TREX1 loss impairs CT26 tumor growth in immunocompetent mice a cGAS dependent manner.</p>
<p>Supplementary Figure 4. Single-cell RNA sequencing workflow and ISG expression across immune cells in the tumor microenvironment</p>
<p>Supplementary Figure 3. Assessment of TREX1’s catalytic activity and cGAS dependency.</p>
<div>Abstract<p>The DNA exonuclease three-prime repair 1 (TREX1) is critical for preventing autoimmunity in mice and humans by degrading endogenous cytosolic DNA, which otherwise triggers activation of the innate cGAS/STING pathway leading to production type I IFNs. As tumor cells are prone aberrant accumulation, we hypothesized that they critically dependent on TREX1 activity limit their immunogenicity. Here, show cells, restricts spontaneous pathway, subsequent induction a IFN...
A critical barrier to the development of a human immunodeficiency virus (HIV) cure is lack scalable animal model that enables robust evaluation eradication approaches prior testing in humans. We established humanized mouse latent HIV infection by transplanting "J-Lat" cells, Jurkat cells harboring provirus encoding an enhanced green fluorescent protein (GFP) reporter, into irradiated adult NOD.Cg-Prkdc