A5026985599- interferon and immune responses
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- Cell death mechanisms and regulation
- RNA Research and Splicing
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Bacterial Infections and Vaccines
- Immune Response and Inflammation
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Alzheimer's disease research and treatments
- Monoclonal and Polyclonal Antibodies Research
- Nanofabrication and Lithography Techniques
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- RNA Interference and Gene Delivery
University of Wollongong
2024
The University of Sydney
2018-2022
Herpesviruses are known to encode a number of inhibitors host cell death, including RIP Homotypic Interaction Motif (RHIM)-containing proteins. Varicella zoster virus (VZV) is member the alphaherpesvirus subfamily and responsible for causing chickenpox shingles. We have identified novel viral RHIM in VZV capsid triplex protein, open reading frame (ORF) 20, that acts as death inhibitor. Like human cellular RHIMs RIPK1 RIPK3 stabilise necrosome TNF-induced necroptosis, M45 from murine...
Quenchbodies are antibodies labelled with a fluorophore that increases in fluorescence intensity upon antigen binding, which makes them promising candidates for the development of diagnostic or other immunoassays requiring quantification. based on camelid nanobodies particularly attractive rapid against molecular antigens interest, due to their small size, ease expression, high stability, evolvability, and amenability protein engineering. However, current nanobody-based quenchbodies display...
TIR-domain-containing adapter-inducing interferon-β (TRIF) is an innate immune protein that serves as adaptor for multiple cellular signalling outcomes in the context of infection. TRIF activated via ligation Toll-like receptors 3 and 4. One outcome TRIF-directed activation programmed cell death pathway necroptosis, which governed by interactions between proteins contain a RIP Homotypic Interaction Motif (RHIM). contains RHIM sequence can interact with receptor interacting kinases 1 (RIPK1)...
Abstract The M45 protein from murine cytomegalovirus protects infected cells death by necroptosis and can protect human induced TNFR activation, when heterologously expressed. We show that the N-terminal 90 residues of protein, which contain a RIP Homotypic Interaction Motif (RHIM), are sufficient to confer protection against TNFR-induced necroptosis. This region drives rapid self-assembly into homo-oligomeric amyloid fibrils interacts with RHIMs RIPK1 RIPK3 kinases form heteromeric in vitro...