A5106367183- Carbohydrate Chemistry and Synthesis
- Lysosomal Storage Disorders Research
- Trypanosoma species research and implications
- Glycosylation and Glycoproteins Research
Leiden University
2023-2025
Macrophages are specialised cells that degrade a range of substrates during their lifetime. In inherited lysosomal storage disorders, particularly the sphingolipidoses, macrophages transform into and contribute to pathology. An appropriate cultured macrophage model is desired for fundamental research assessment considered therapeutic interventions. We compared commonly used cell lines, RAW264.7, J774A.1, THP-1 cells, with human monocyte-derived (HMDMs) isolated from peripheral blood....
Parallel FluoPol-ABPP screenings on lysosomal β-glucosidase (GBA1) and α-glucosidase (GAA) revealed a N -9-phenanthrenyl-DNJ that inhibits GAA selectively is an interesting hit for the development of chaperones Pompe disease.
Fluorescent β- d -arabinofuranosyl cyclitol aziridine activity-based probes potently and selectively label GBA2 both in vitro cellulo , allowing visualization of overexpressed using fluorescent microscopy.