A5093567998- Parkinson's Disease Mechanisms and Treatments
- Autophagy in Disease and Therapy
- Fungal and yeast genetics research
- Protein Tyrosine Phosphatases
- Mitochondrial Function and Pathology
Research Network (United States)
2024
Universitätsmedizin Göttingen
2023-2024
University of Dundee
2024
University of Göttingen
2023-2024
Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103
2024
Loss-of-function mutations in PTEN-induced kinase 1 (PINK1) are a frequent cause of early-onset Parkinson’s disease (PD). Stabilization PINK1 at the translocase outer membrane (TOM) complex damaged mitochondria is critical for its activation. The mechanism how activated TOM unclear. Here, we report that co-expression human and all seven subunits Saccharomyces cerevisiae sufficient We use this reconstitution system to systematically assess role each subunit toward unambiguously demonstrate...
The PINK1-Parkin axis plays a major role in mitochondrial quality control and mutations have been closely associated with familial cases of Parkinson's disease [1]. To assess the correct functioning mitochondria, PINK1 acts as sensor by monitoring their import capabilities [2]. While proper leads to degradation PINK1, failed causes form complex translocon outer membrane, allowing it be stabilized activated cross- phosphorylation [3]. Here we describe protocol for live-cell imaging...
Abstract Loss of function mutations in PTEN-induced kinase 1 (PINK1) are a frequent cause early-onset Parkinson’s disease (PD). Stabilisation PINK1 at the Translocase Outer Membrane (TOM) complex damaged mitochondria is critical step for its activation. To date mechanism how activated TOM unclear. Herein we report co-expression human and all seven subunits Saccharomyces cerevisiae sufficient We use this reconstitution system to systematically assess role each subunit towards unambiguously...