Abstract STUDY QUESTION Is a microfluidic sperm sorter (MSS) able to select higher quality compared conventional methods? SUMMARY ANSWER The MSS selects with improved parameters, lower DNA fragmentation, and fertilizing potential. WHAT IS KNOWN ALREADY To date, the few studies that have microfluidics selection methods used heterogeneous study population lacked molecular investigations. DESIGN, SIZE, DURATION efficiency of newly designed in isolating high-quality was density-gradient...

Infertility is inability to conceive after 12 months of regular unprotected sex. MiRNA expression changes can serve as potential biomarkers for infertility in males due impaired spermatogenesis. This research was conducted measure the level miR-211 plasma samples a factor identifying comparison with control group.

Background . Interferon gamma (IFN- γ ) is an immune regulatory cytokine that acts through its receptor and plays important role in progression of inflammatory disease such as chronic periodontitis (CP). The purpose this study was to determine the differences distribution IFN- (+874A/T) R1 (-611A/G, +189T/G, +95C/T) gene polymorphisms among CP healthy individuals investigate relationships between these susceptibility CP. Materials Methods 310 were enrolled including 210 patients 100...

Maternal smoking during pregnancy can induce structural and functional changes in the placenta. Placentas from heavy smoker (>20 cigarettes per day) mothers non-smoker healthy controls (n=10 each group) were enrolled present case-control study. Sample selection sectioning performed by systematic uniform random sampling (SURS). Selected sections stained using Masson's trichrome to estimate quantitative parameters of placental extravillous trophoblast cells (EVTs) number EVTs. Differences...

Smoking during pregnancy is able to alter the structure and function of placenta. In present study, quantitative changes placenta in smoking mothers were investigated compared healthy controls by Cavalieri's point counting method.Twenty placentas from heavy non-smoker (n = 10 each group) selected. Systematic uniform random sampling (SURS) was used for sample selection tissue sectioning. Quantitative parameters selected sections estimated after Masson's trichrome staining. Differences between...

In the present study, quantitative changes of extravillous trophoblast cells (EVTs) in placentas SLE patients were investigated compared to healthy controls using stereological methods. Volumetric parameters and number EVTs per unit volume placenta estimated respectively, Cavalieri's principle Physical Disector Placental group was increased control group, but this increase not statistically significant (p > .05). weight patient showed a decrease < Total EVTs, diameter nucleus cytoplasm N/C...

The quantitative changes of extravillous trophoblast cells (EVTs) in placenta gestational diabetes mellitus (GDM) patients were investigated compared to healthy controls using stereological methods. volumetric parameters the and EVTs estimated Cavalieri's principle Physical Disector placental volume weight GDM group increased control (p < .05). difference total intervillous space blood vessels between groups was statistically significant In addition, there a density syncytiotrophoblast EVTs,...

Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran Department Histology, School Medicine, Corresponding author: Nadia Sheibak, Iran. Tel: +98-5433295794, Fax: +98-5433295794. E-mail: nadia1989sh@yahoo.com

Chronic periodontitis (CP), an infectious disease resulting in inflammation within the periodontal tissue, is main cause of adult tooth loss. CP a multi-factorial disorder and interaction between multiple genetic environmental factors results manifestation this disease. Recent researches has focused on cytokine gene polymorphisms that play important role inflammation, but few studies investigated histological change occur during supporting tissue teeth. The aims study were to investigate...

Background: Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator. It plays vital role in immune response against the oral disease. MIF regulator of innate immunity, and bacterial antigens can stimulate serum level this protein. In experimental gingivitis, expression increases increment positively correlates with plaque index. The single nucleotide polymorphisms gene encoding protein control function MIF. aim present study was clarification associations between...

The present study investigated the effects of DNA fragmentation spermatozoa on growth factors expression by a human oviduct epithelial cell line (OE-E6/E7). Two separate groups were examined in this study. was cultured presence with normal index (DFI) or abnormal DFI. Total RNA from each group isolated, and relative objective genes analysed using PCR array. Also, concentration VEGF, BMP-2, BMP-7 MSTN supernatant culture ELISA method. array analysis revealed that most had been upregulated...

We evaluated the expression of interleukin-22 (IL-22) in endometrium women with unexplained recurrent pregnancy loss (uRPL) and infertility (UI) compared to normal pregnancies. Endometrial tissues were collected from 20 UI, uRPL, 24 healthy as a control group. Immunohistochemical gene IL-22 analyzed by immunohistochemistry (IHC) quantitative reverse transcription-polymerase chain reaction (qRT-PCR) methods. The controls showed lower than uRPL group (P > 0.05) using PCR. It was also found...

Background: The recent years has seen enormous research attempts in the study for genetic factors that may predispose individuals to periodontitis, as one of most common chronic infectious disease humans. Objectives: aim present was determine breakdown interdental gingiva tissues periodontitis patients with TGF-β1 (-509) C/T polymorphisms. Patients and Methods: A total 60 gingival samples, including 45 patient 15 healthy subjects, were selected this study. Following determination gene...

Background: The association between interleukin-6 (IL-6) (-174G/C) gene polymorphisms and level of tissue breakdown periodontal disease progression is unknown. present study investigated quantitative parameters interdental papilla in chronic periodontitis (CP) patients with IL-6 polymorphisms. Materials Methods: Sixty gingival samples were studied. After determination using a tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) technique, 45 CP (GG...

We undertook this study to determine quantitative changes of the placenta, focusing on extravillous trophoblastic cells (EVTs) in pregnancies with intrauterine growth restriction (IUGR) and small for gestational age (SGA) compared control group.Placentas from complicated SGA-IUGR (n = 10) group were obtained after cesarean surgery evaluated using stereological assays routine tissue processing Masson's trichrome staining. Mann-Whitney U-test was employed, level statistical significance set at...

Hypoxia is a critical part of normal placentation characterized by pO 2 < 20 mmHg in the placenta during 8 th to 10 weeks pregnancy (1, 2).

Abstract Aim Ectopic pregnancy (EP) is one of the most important causes maternal mortality. This study aimed to evaluate immunohistochemical (IHC) expression cannabinoid receptor type‐1 (CB1) and its association with CB1 ‐ 1359G/A gene polymorphism (rs1049353) in fallopian tubes EP compared controls. Methods In this case–control study, 100 women (cases) that underwent abdominal surgery due hysterectomy or uterine tubal ligation (healthy controls) were included. Genotyping CB1‐1359G/A...