Excess cyclin E-Cdk2 accelerates entry into S phase of the cell cycle and promotes polyploidy, which may contribute to genomic instability in cancer cells. We identified 20 amino acids E as a centrosomal localization signal (CLS) essential for both targeting promoting DNA synthesis. Expressed wild-type, but not mutant, CLS peptides localized on centrosome, prevented endogenous A from localizing inhibited Ectopic centrosome accelerated even with mutations that abolish Cdk2 binding, mutation...

Pulmonary lymphangioleiomyomatosis (LAM), a disease of young women, is characterized by proliferation immature-appearing smooth-muscle cells (LAM cells) in the lungs and abdomen. LAM react with monoclonal antibody HMB45, which recognizes 100-kD glycoprotein (gp100) originally found human melanoma cells. We investigated expression subcellular localization gp100 lung tissue from patients cell lines (Malme-3M, A2058, CHL-1), relationship between this cellular proliferation. Binding sites for...

Aberrant centrosome duplication is observed in many tumor cells and may contribute to genomic instability through the formation of multipolar mitotic spindles. Cyclin-dependent kinase 2 (Cdk2) required for multiple rounds Xenopus egg extracts but not initial round replication. Egg undergo periodic oscillations level free calcium. We show here that chelation calcium or specific inactivation calcium/calmodulin–dependent protein II (CaMKII) blocks even duplication, whereas Cdk2 does not....

Circular RNA replicons have been reported in plants and, one case, animal cells. We describe such an element yeast. In certain yeast strains, a 20S species appears on transfer of cells to acetate medium. This phenotype shows cytoplasmic (non-Mendelian) inheritance and the is associated with 23-kDa protein subunits as 32S particle. demonstrate that independent replicon no homology host genomic, mitochondrial, or 2-microns plasmid DNA L-A, L-BC, M1 double-stranded viruses The circularity shown...

Urban particulate air pollution is associated with an increased incidence of cancers, and especially lung cancer. Organic extracts airborne matter (APM) cause cancer in mice, PAHs adsorbed to APM are particle-induced carcinogenesis. agonists for AhR predominantly responsible through induction highly carcinogenic metabolites. PAH metabolization requires CYP1A1 activation AhR, therefore we hypothesized that carcinogenesis due would be reduced AhR-/- mice. To examine this hypothesis, performed...

RUNX3 is a novel tumor suppressor gene that frequently silenced by promoter hypermethylation in gastric cancer. The methylation status of multiple regions within the CpG island (3478 bp) was examined cancer cell lines, primary cancers and non‐neoplastic mucosa to clarify how spreads island. critical for silencing were evaluated analysis lines. most 5′ region methylated 90% (9/10) 96% (43/45) mucosa. frequencies less near transcription start site 40% (4/10) 53% (24/45) 11% (5/45) mucosa,...

Inhibition of GPVI has been proposed as a useful antithrombotic strategy; however, in vivo proof-of-concept animal studies targeting are lacking. We evaluated novel anti-human monoclonal antibody OM4 Fab rats.OM4 specifically inhibited collagen-induced aggregation rat platelets vitro with an IC50 20 to 30 microg/mL but not ADP and AA-induced platelet aggregation. After intravenous administration Fab, rapid inhibition ex was observed gradual recovery within 60 90 minutes which corresponded...

To prevent membrane fouling, cross-flow microfiltration with ultrasonic wave cleaning was carried out using a model suspension containing both baker's yeast and bovine serum albumin (BSA). The effects of waves on the permeate flux rejection BSA were studied.The steady-state obtained in filtration four to six times greater than that without waves, high obtained, even at low feed flow velocity. There an optimum pore size operating pressure. It found very effective for removing cake layer...

The aim of this study was to evaluate and compare the effects anti-platelet agents with different modes action (cilostazol, aspirin, clopidogrel) on brain infarction produced by photothrombotic middle-cerebral-artery (MCA) occlusion in male, spontaneously hypertensive rats. Cerebral blood flow (CBF) measured laser-Doppler flowmetry penumbral cortex. Infarct size evaluated 24 h after MCA occlusion. these drugs infarct were examined pretreatment rats undergoing Pretreatment cilostazol (100...

Airborne particulates were collected at a site near group of factories in Kitakyushu, one the most industrialized cities Japan, and downtown sites Sapporo Tokyo, typical large commercial cities. We determined levels polycyclic aromatic hydrocarbons (PAHs) nitropolycyclic (NPAHs), which are known to be carcinogenic and/or mutagenic. The atmospheric concentrations PAHs with 4-, 5-, 6-rings higher Kitakyushu Tokyo than Sapporo. On other hand, strongly mutagenic NPAHs highest Sapporo,...

Background and objectives: Aroma therapy is a complementary using essential oils diluted with carrier oils. Jojoba have been widely used as However, limited information available regarding their effects on blood biochemical parameters. This study aimed to investigate the effect of transdermal administration jojoba oil parameters in mice. Materials Methods: Eight-week-old male hairless mice were randomly divided into naïve control treatment groups. In group, topically administered 4 μL oil,...

Air samples were collected at the sides of busy traffic roads in downtown Kanazawa, Sapporo and Tokyo during same periods both daytime night, winter summer. We determined levels polycyclic aromatic hydrocarbons (PAHs) nitropolycyclic (NPAHs), which are known to be carcinogenic and/or mutagenic. PAHs NPAHs analyzed simultaneously with an HPLC system fluorescence detection for another chemiluminescence NPAHs, respectively. The amounts airborne particulates larger than night all three cities....

The Ames Salmonella mutagenicity bioassay using strains TA98 and TA100 of airborne particulates from Sapporo, Japan, was studied over an 18-year period starting in 1974. Measurements benzo[a]pyrene (BaP), as well other polycyclic aromatic hydrocarbones (PAHs), were performed on organic extracts which prepared seasonal particulate composites high-volume filter samples for each year. average contribution BaP to total indirect-acting mutagenic activity 1.8% strain 3.5% TA100. During the...

Abstract The 20 S RNA genome is a circular single-stranded replicon, present in most laboratory yeast strains, whose copy number induced 10,000-fold by transfer of cells to acetate medium without carbon source. We have sequenced the genome, and (+) strand has long open reading frame with potential encode protein homology viral RNA-dependent polymerases. presence typical cAMP-dependent phosphorylation site putative polymerase suggests that amplification might be mediated cAMP, signal known...

Two new prenylflavones, cudraflavones C and D, along with six know compounds were isolated from the root bark of Cudrania tricuspidata (Carr.) Bur. (Moraceae), collected in China. The structures D elucidated on basis spectroscopic data