A5102833536- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
Princess Margaret Cancer Centre
2023-2024
University Health Network
2023-2024
University of Toronto
2023-2024
Heterotrimeric G proteins can be regulated by posttranslational modifications, including ubiquitylation. KCTD5, a pentameric substrate receptor protein consisting of an N-terminal BTB domain and C-terminal domain, engages CUL3 to form the central scaffold cullin-RING E3 ligase complex (CRL3 KCTD5 ) that ubiquitylates Gβγ reduces levels in cells. The cryo-EM structure 5:5:5 KCTD5/CUL3 NTD /Gβ 1 γ 2 assembly reveals highly dynamic with rotations over 60° between /CUL3 CTD /Gβγ moieties...
Abstract Heterotrimeric G proteins can be regulated by post-translational modifications, including ubiquitylation. KCTD5, a pentameric substrate receptor protein consisting of an N-terminal BTB domain and C-terminal (CTD), engages CUL3 to form the central scaffold cullin- RING E3 ligase complex (CRL3 KCTD5 ) that ubiquitylates Gβγ reduces levels in cells. The cryo-EM structure 5:5:5 KCTD5/CUL3 NTD /Gβ 1 γ 2 assembly reveals highly dynamic with rotations over 60° between /CUL3 CTD /Gβγ...