A5068977419- Cancer, Hypoxia, and Metabolism
- Receptor Mechanisms and Signaling
- Ion Transport and Channel Regulation
- Sphingolipid Metabolism and Signaling
- Helicobacter pylori-related gastroenterology studies
- Nitric Oxide and Endothelin Effects
- Drug Transport and Resistance Mechanisms
- Cell Image Analysis Techniques
- Cancer Immunotherapy and Biomarkers
- Cancer, Lipids, and Metabolism
- Cell Adhesion Molecules Research
- Chemical Synthesis and Analysis
- ATP Synthase and ATPases Research
- T-cell and B-cell Immunology
- Cancer therapeutics and mechanisms
- Epigenetics and DNA Methylation
- Adenosine and Purinergic Signaling
- Gastroesophageal reflux and treatments
- Forest, Soil, and Plant Ecology in China
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- Erythrocyte Function and Pathophysiology
- Gut microbiota and health
- Plant Ecology and Soil Science
- Image Processing Techniques and Applications
Sichuan University
2012-2025
East Carolina University
2015-2024
Nanjing Medical University
2024
Jiangsu Province Hospital
2024
Capital Institute of Pediatrics
2024
Cincinnati Children's Hospital Medical Center
2015-2022
State Key Laboratory of Biotherapy
2011-2021
Chengdu University
2017-2021
Kent State University
2020
University of Alabama
2020
Migration is a key property of live cells and critical for normal development, immune response, disease processes such as cancer metastasis inflammation. Methods to examine cell migration are very useful important wide range biomedical research biology, immunology, vascular biology developmental biology. Here we use tumor invasion an example describe two related assays illustrate the commonly used, easily accessible methods measure these processes. The first method culture wound closure...
Phagocytosis of apoptotic cells is fundamentally important throughout life, because non-cleared become secondarily necrotic and release intracellular contents, thus instigating inflammatory autoimmune responses. Secreted "find-me" exposed "eat-me" signals displayed by the dying cell in concert with phagocyte receptors comprise phagocytic synapse clearance. In this scenario, lysophospholipids (lysoPLs) are assumed to act as find-me for attraction phagocytes. However, both identity lyso-PLs...
G2A is an immunoregulatory G protein-coupled receptor predominantly expressed in lymphocytes and macrophages. Ectopic overexpression studies have implicated as a for the bioactive lysophospholipid, lysophosphatidylcholine (LPC). However, functional consequences of LPC–G2A interaction at physiological levels expression, cellular context relevant to its immunological role, remain largely unknown. Here, we show impaired chemotaxis LPC T lymphoid cell line which expression was chronically...
Acidic tissue microenvironment commonly exists in inflammatory diseases, tumors, ischemic organs, sickle cell disease, and many other pathological conditions due to hypoxia, glycolytic metabolism deficient blood perfusion. However, the molecular mechanisms by which cells sense respond acidic are not well understood. GPR4 is a proton-sensing receptor expressed endothelial types. The fully activated extracellular pH but exhibits lesser activity at physiological 7.4 minimal more alkaline pH. To...
Endothelium-leukocyte interaction is critical for inflammatory responses. Whereas the tissue microenvironments are often acidic at sites, mechanisms by which cells respond to acidosis not well understood. Using molecular, cellular and biochemical approaches, we demonstrate that activation of GPR4, a proton-sensing G protein-coupled receptor, isocapnic increases adhesiveness human umbilical vein endothelial (HUVECs) express GPR4 endogenously. Acidosis in combination with overexpression...
Background Systemic immune activation, hallmarked by C-reactive protein (CRP) and interleukin-6 (IL-6), can modulate antitumor responses. In this study, we evaluated the role of IL-6 CRP in stratification patients with non-small cell lung cancer (NSCLC) treated checkpoint inhibitors (ICIs). We also interrogated underlying immunosuppressive mechanisms driven IL-6/CRP axis. Methods cohort A (n=308), estimated association baseline objective response rate (ORR), progression-free survival (PFS),...
GPR4 is a G protein-coupled receptor expressed in the vasculature, lung, kidney, and other tissues.In vitro ectopic overexpression studies implicated sensing extracellular pH changes leading to cyclic AMP (cAMP) production.To investigate its biological roles vivo, we generated GPR4-deficient mice by homologous recombination.Whereas GPR4-null adult appeared phenotypically normal, neonates showed higher frequency of perinatal mortality.The average litter size from ؊/؊ intercrosses was ϳ30%...
Proton receptors are G protein-coupled that accept protons as ligands and function pH sensors. One of the proton receptors, GPR4, is relatively abundant in kidney, but its potential role acid-base homeostasis unknown. In this study, we examined distribution GPR4 kidney epithelial cells, effects deletion on homeostasis. We observed expression cortex, outer inner medulla, isolated collecting ducts, cultured medullary duct cells (mOMCD1 mIMCD3). Cultured mOMCD1 exhibited pH-dependent...
No prediction rule is currently available for advanced colorectal neoplasms, defined as invasive cancer, an adenoma of 10 mm or more, a villous adenoma, with high-grade dysplasia, in average-risk Chinese. In this study between 2006 and 2008, total 7,541 Chinese persons aged 40 years older who had complete colonoscopy were included. The derivation validation cohorts consisted 5,229 2,312 persons, respectively. A was developed from logistic regression model then internally externally...
Acidosis commonly exists in the tissue microenvironment of various pathophysiological conditions such as tumors, inflammation, ischemia, metabolic disease, and respiratory disease. For instance, tumor is characterized by acidosis hypoxia due to heterogeneity, aerobic glycolysis (the "Warburg effect"), defective vasculature that cannot efficiently deliver oxygen nutrients or remove acid byproduct. How acidic affects function blood vessels, however, not well defined. GPR4 (G protein-coupled...