A5017994209- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Enzyme Structure and Function
- Redox biology and oxidative stress
- Protein Structure and Dynamics
- Bioinformatics and Genomic Networks
- Heat shock proteins research
- Silk-based biomaterials and applications
- Supramolecular Self-Assembly in Materials
- Selenium in Biological Systems
- Sulfur Compounds in Biology
- Advanced Proteomics Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Materials and Mechanics
- Monoclonal and Polyclonal Antibodies Research
ETH Zurich
2016-2025
Systems Technology (United States)
2024
ZHAW Zurich University of Applied Sciences
2023
Cells react to extracellular perturbations with complex and intertwined responses. Systematic identification of the regulatory mechanisms that control these responses is still a challenge requires tailored analyses integrating different types molecular data. Here we acquired time-resolved metabolomics measurements in yeast under salt pheromone stimulation developed machine learning approach explore associations between metabolism signal transduction. Existing phosphoproteomics same...
Abstract Protein serine/threonine/tyrosine (S/T/Y) phosphorylation is an essential and frequent post-translational modification in eukaryotes, but historically has been considered less prevalent bacteria because fewer proteins were found to be phosphorylated most modified a lower degree. Recent proteomics studies greatly expanded the phosphoproteome of Escherichia coli more than 2000 sites (phosphosites), yet mechanisms action proposed for only six phosphosites fitness effects described 38...
Designing proteins that fold and assemble over different length scales provides a way to tailor the mechanical properties biological performance of resulting hydrogels. In this study, we designed modular self-assemble into fibrillar networks and, as result, form hydrogel materials. We incorporated distinct functionalities by connecting separate self-assembling (A block) cell-binding (B domains single macromolecules. The number affects rigidity fibers final storage modulus G′ hydrogels could...
Coordinated through a complex network of kinases and phosphatases, protein phosphorylation regulates essentially all cellular processes in eukaryotes. Recent advances proteomics enable detection thousands sites (phosphosites) single experiments. However, functionality the vast majority these remains unclear we lack suitable approaches to evaluate functional relevance at pace that matches their detection.Here, assess 26 phosphosites by introducing phosphodeletion phosphomimic mutations 25...
Abstract The redox state of proteins is essential for their function and guarantees cell fitness. Peroxiredoxins protect cells against oxidative stress, maintain homeostasis, act as chaperones transmit hydrogen peroxide signals to regulators. Despite the profound structural functional knowledge peroxiredoxins action, information on how different functions are concerted still scare. Using global proteomic analyses, we show here that yeast peroxiredoxin Tsa1 binds hundreds biological...
Preparation of expression vectors using conventional cloning strategies is laborious and not suitable for the design metabolic pathways or enzyme cascades, which usually requires preparation a vector library to identify productive clones. Recently, Modular Cloning as novel technique in synthetic biology has been developed. relies on Golden Gate assembly supports individual one-step one-pot reactions, thus allowing rapid generation libraries. A number toolkits specific applications...