A5017061254- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- DNA Repair Mechanisms
- Cancer, Stress, Anesthesia, and Immune Response
- Cellular transport and secretion
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Polysaccharides and Plant Cell Walls
- Biofuel production and bioconversion
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Estrogen and related hormone effects
- Advanced Cellulose Research Studies
- Adenosine and Purinergic Signaling
- Mitochondrial Function and Pathology
- Plant Genetic and Mutation Studies
- interferon and immune responses
- Microtubule and mitosis dynamics
- Autism Spectrum Disorder Research
Karolinska Institutet
2019-2025
Science for Life Laboratory
2019-2024
University of Zurich
2019
Institute of Technical Chemistry
2014
University of Łódź
2014
Antibodies binding to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike have therapeutic promise, but emerging variants show potential for virus escape. This emphasizes need molecules with distinct and novel neutralization mechanisms. Here we describe isolation of a nanobody that interacts simultaneously two RBDs from different trimers SARS-CoV-2, rapidly inducing formation trimer-dimers leading loss their ability attach host cell receptor, ACE2. We this potently...
<title>Abstract</title> Epithelial-mesenchymal transition (EMT) is a key biological process in development and its reactivation plays an important role cancer progression resistance to various therapies. There lack of EMT targeting strategies current methods assess are limited by low scalability insufficient capacity score capture hybrid states. To address this, we developed novel morphology-based machine learning method predict epithelial-mesenchymal plasticity (EMP) using organelle...
During DNA replication stress, stalled forks need to be stabilized prevent fork collapse and genome instability. The AAA + ATPase WRNIP1 (Werner Helicase Interacting Protein 1) has been implicated in the protection of from nucleolytic degradation, but underlying molecular mechanism remained unclear. Here we show that exerts its protective function downstream reversal. Unexpectedly though, is not part well-studied BRCA2-dependent branch seems protect junction point reversed SLX4-mediated...
Among others, expression levels of programmed cell death 1 ligand (PD-L1) have been explored as biomarkers the response to immune checkpoint inhibitors in cancer therapy. Here, we present results a chemical screen that interrogated how medically approved drugs influence PD-L1 expression. As expected, corticosteroids and Janus kinases were among top downregulators. In addition, identified is induced by antiestrogenic compounds. Transcriptomic analyses indicate chronic estrogen receptor alpha...
Several viruses hijack various forms of endocytosis in order to infect host cells. Here, we report the discovery a molecule with antiviral properties that named virapinib, which limits viral entry by macropinocytosis. The identification virapinib derives from chemical screen using high-throughput microscopy, where identified entities capable preventing infection pseudotype virus expressing spike (S) protein SARS-CoV-2. Subsequent experiments confirmed capacity inhibit SARS-CoV-2, as well...
ABSTRACT Estrogen receptor (ER)-positive breast tumors are routinely treated with estrogen-depriving therapies. Despite their effectiveness, patients often progress into a more aggressive form of the disease. Through chemical screen oriented to identify chemicals capable inducing expression immune-checkpoint ligand PD-L1, we found antiestrogens as hits. Subsequent validations confirmed that estrogen deprivation or ERα depletion induces PD-L1 in ER-positive cancer cells, both vitro and vivo ....
Abstract Antibodies binding to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike have therapeutic promise, but emerging variants show potential for virus escape. This emphasizes need molecules with distinct and novel neutralization mechanisms. Here we isolated a nanobody that interacts simultaneously two RBDs from different trimers of SARS-CoV-2, rapidly inducing formation trimer-dimers leading loss their ability attach host cell receptor, ACE2. We this potently...
Speed is key during infectious disease outbreaks. It essential, for example, to identify critical host binding factors pathogens as fast possible. The complexity of plasma membrane often a limiting factor hindering and accurate determination well high-throughput screening neutralizing antimicrobial drug targets. Here, we describe multiparametric platform tackling this bottleneck enabling screens new antiviral sensitivity robustness our were validated by blocking SARS-CoV-2 particles with...
The tetracycline repressor (tetR)-regulated system is a widely used tool to specifically control gene expression in mammalian cells. Based on this system, we generated human osteosarcoma cell line, which allows for the inducible of an EGFP fusion TAR DNA-binding protein 43 (TDP-43), has been linked neurodegenerative diseases. Consistent with previous findings, TDP-43 overexpression led accumulation aggregates and limited viability U2OS. Using conducted chemical screen library that included...
The presentation of neoantigens by HLA-I is essential for the recognition tumor cells cytotoxic T cells. Transcriptionally, expression regulated interferon-dependent activation JAK/STAT signaling. Accordingly, mutations that inactivate this pathway are one main causes resistance to cancer immunotherapies. Recent evidences indicate can be induced independently IFN-signaling innate immune response. In context, we performed an image-based screen evaluate how more than 5,000 chemicals, including...
SUMMARY Several viruses hijack various forms of endocytosis in order to infect host cells. Here, we report the discovery a new molecule with antiviral properties that named virapinib, which limits viral entry by macropinocytosis. The identification virapinib derives from chemical screen using High-Throughput Microscopy, where identified entities capable preventing infection pseudotype virus expressing spike (S) protein SARS-CoV-2. Subsequent experiments confirmed capacity inhibit SARS-CoV-2,...
ABSTRACT The tetracycline repressor (tetR)-regulated system is a widely used tool to specifically control gene expression in mammalian cells. Based on this system, we generated human osteosarcoma cell line which allows for inducible of an EGFP-fusion the TAR DNA-binding protein 43 (TDP-43), has been linked neurodegenerative diseases. Consistent with previous findings, TDP-43 overexpression led accumulation aggregates and limited viability U2OS. Using conducted chemical screen library that...
Abstract Speed is key during infectious disease outbreaks. It essential, for example, to identify critical host binding factors the pathogens as fast possible. The complexity of plasma membrane often a limiting factor hindering and accurate determination well high-throughput screening neutralizing antimicrobial drug targets. Here we describe multi-parametric platform tackling this bottleneck enabling screens new antiviral sensitivity robustness our was validated by blocking SARS-CoV-2 spike...