A5014944735- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Digital Holography and Microscopy
- Neural dynamics and brain function
- Seismic Imaging and Inversion Techniques
- Memory and Neural Mechanisms
- Hydraulic Fracturing and Reservoir Analysis
- Image Processing Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Geophysical and Geoelectrical Methods
- EEG and Brain-Computer Interfaces
- Hydrocarbon exploration and reservoir analysis
- Cell Image Analysis Techniques
- Seismic Waves and Analysis
- Advanced X-ray Imaging Techniques
University of Virginia
2022
Second Affiliated Hospital of Zhejiang University
2018-2021
Zhejiang University
2018-2020
State Key Laboratory of Modern Optical Instruments
2019
Tarim University
2014
Significance Most biological tissue is opaque, and the inside fine structures are invisible even with a microscope. Optical clearing methods can reduce scattering enable us to “look” samples. Current optical suffer from large time consumption, distortion, fluorescence quenching, which greatly limit their applications. Here, we describe nontoxic ultrafast method (FOCM), could clarify 300-µm-thick brain slices within 2 min without morphology distortion. The be preserved up 86% after 11 d....
Non-invasive, real-time imaging and deep focus into tissue are in high demand biomedical research. However, the aberration that is introduced by refractive index inhomogeneity of biological hinders way forward. A rapid focusing with sensor-less corrections, based on machine learning, demonstrated this paper. The proposed method applies Convolutional Neural Network (CNN), which can rapidly calculate low-order aberrations from point spread function images Zernike modes after training. results...
Abstract The amygdala, one of the most studied brain structures, integrates brain-wide heterogeneous inputs and governs multidimensional outputs to control diverse behaviors central survival, yet how amygdalar input-output neuronal circuits are organized remains unclear. Using a simplified cell-type- projection-specific retrograde transsynaptic tracing technique, we scrutinized afferent four major output groups in basolateral complex (BLA) that project bed nucleus stria terminals (BNST),...
Two‐photon microscopy (2PM) is one of the most widely used tools for in vivo deep tissue imaging. However, spatial resolution and penetration depth are still limited due to strong scattering background. Here we demonstrate a two‐photon focal modulation microscopy. By utilizing demodulation techniques, background rejection capability enhanced, thus imaging improved. Compared with 2PM, transverse increased by 70%, while axial 2‐fold. Furthermore, when applied conventional 2PM mode, it can...
The combination of light sheet fluorescence microscopy (LSFM) and the optical clearing method can achieve fast three-dimensional high-resolution imaging. However, there is an essential contradiction between field view (FoV) spatial resolution. Also, aberration scattering still exist after tissue clearing, which seriously limits imaging depth LSFM. Here we propose a Schwartz modulation implement it in LSFM based on quasi-Bessel beam to enlarge FoV without sacrificing its simulation results...
Intercellular communication mediated by a large number of neuromodulators diversifies physiological actions, yet neuromodulation remains poorly understood despite the recent upsurge genetically encoded transmitter sensors. Here, we report development versatile sensor-based image analysis program (GESIAP) that utilizes MATLAB-based algorithms to achieve high-throughput, high-resolution processing functional imaging data. GESIAP enables delineation fundamental properties (e.g., spatial...
We introduce a new vector-imaging condition for elastic reverse-time migration to produce high-resolution images. This imaging is developed by the use of polarization vectors wave fields along with conventional condition. The as that used scalar-wave migration, established on principle incident and reflected waves coincide in phase at reflection point. Our also makes directional coincidence forward backward propagating waves. are obtained spatial derivatives corresponding frequency domain....
Summary We present results from a case study being performed in the HS area of Tarim basin that utilizes two orthogonal Wide Azimuth (WAZ) surveys to create an improved image subsurface. demonstrate WAZ acquisition – acquiring second dataset over existing data provides efficient way obtain near Full (FAZ) coverage at relatively low cost. By using offset vector tile (OVT) technology, we also considerable uplift imaging target carbonate reservoir and facilitates application multi azimuthal...