A5035562080- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Environmental DNA in Biodiversity Studies
- Parasitic Infections and Diagnostics
- Aquatic Invertebrate Ecology and Behavior
- Parasite Biology and Host Interactions
- Molecular Sensors and Ion Detection
- Methane Hydrates and Related Phenomena
- Marine and coastal ecosystems
- Luminescence and Fluorescent Materials
- Marine Biology and Ecology Research
- Advanced biosensing and bioanalysis techniques
University of Duisburg-Essen
2020-2024
University of Cologne
2024
Comenius University Bratislava
2014-2019
Abstract Members of the class Armophorea occur in microaerophilic and anaerobic habitats, including digestive tract invertebrates vertebrates. Phylogenetic kinships metopid clevelandellid armophoreans conflict with traditional morphology‐based classifications. To reconcile their relationships understand morphological evolution diversification, we utilized molecular clock theory as well information contained estimated time trees morphology extant taxa. The radiation last common ancestor...
Reconstruction of evolutionary history the ciliate order Spathidiida has proven elusive. There are weakly statistically supported deeper nodes in 18S rRNA gene phylogenies on one hand, while several strongly clusters containing morphologically dissimilar taxa that usually lack any common apomorphies other hand. To clarify whether silent aspects spathidiid phylogeny results methodological problems tree‐building algorithms, we examined informativness macronuclear locus using an increased taxon...
A library of eight different cationic emitters with emission properties in solution and solid-state (solution - SSSE) is presented. These compounds, bearing either ammonium or pyridinium groups, have been investigated regarding their photophysical as well potential application biological imaging. Besides high quantum yields a degree stability during the imaging process, it was additionally revealed that broad range targets can be addressed, such bacterial strains, human cells protists. The...
We investigated interrelationships between four free-living litostomatean lineages, using 18S rRNA gene and ITS region sequences as well the secondary structure of ITS2 molecules. Our phylogenetic analyses confirmed deep split litostomateans into Rhynchostomatia Haptoria represented here by Haptorida, Pleurostomatida, Spathidiida. This bifurcation is also corroborated signature rhynchostomatian haptorian Specifically, consensus stems helices II III are longer one base pair in...
Ciliates are a suitable microbial model to investigate trait-dependent diversification because of their comparatively complex morphology and high diversity. We examined the impact seven intrinsic traits on speciation, extinction, net-diversification rhynchostomatians, group large, predatory ciliates with proboscis carrying dorsal brush (sensoric structure) toxicysts (organelles used kill prey). Bayesian estimates under binary-state speciation extinction indicate that two types extrusomes...
Although ciliates are one of the most dominant microbial eukaryotic groups in many environments, there is a lack updated global ciliate alignments and reference trees that can be used for phylogenetic placement methods to analyze environmental metabarcoding data. Here we fill this gap by providing those taxa with available SSU-rDNA sequences derived from identified species. Each alignment contains 478 six outgroup taxa, they were made using different masking strategies positions (unmasked,...
Ciliates have a long history of being central in evolutionary and ecological studies on eukaryotic microorganisms. Although thousands species been discovered, their total diversity still remains unknown. Here, we will discuss two unsolved problems that hinder the further exploration ciliate at level, potential solutions to these are proposed. First, morphospecies difficult identify because different silver stains not scalable (they do represent high-throughput methods) basic supplies lacking...
The morphology and phylogenetic position of a haptorian ciliate, Phialina pupula (Müller, 1773) Foissner, 1983, isolated from microaerobic sandy sediments the floodplain area Boise River, Idaho, U.S.A., were studied using live observation, protargol impregnation, scanning electron microscopy, 18S rRNA gene as well ITS region. population P. is characterized by size about 60–130 × 20–50 μm, an elliptical macronucleus with single micronucleus, highly refractive dumbbell-shaped inclusions...
Pleurostomatids are predatory ciliates, living especially in the periphyton and benthos of various freshwater marine habitats. In present work, we provide an annotated revised checklist this ciliate group from territory Slovakia. Altogether 29 pleurostome species belonging to five genera have been reported there: Acineria incurvata, Ac. punctata, uncinata, Amphileptus claparedii, Am. falcatus, fusiformis, parafusidens, pleurosigma, procerus, punctatus, A. rotundus, Litonotus alpestris, Li....
Short-branch Microsporidia were previously shown to form a basal grade within the expanded clade and branch near classical, long-branch Microsporidia. Although they share simpler versions of some morphological characteristics, do not show accelerated evolutionary rates, making them ideal candidates study trajectories that has led microsporidians unique characteristics. However, most sequences assigned short-branch are undescribed, novel environmental lineages for which identification...
Abstract Taxonomic assignment of operational taxonomic units (OTUs) is an important bioinformatics step in analyzing environmental sequencing data. Pairwise alignment and phylogenetic‐placement methods represent two alternative approaches to assignments, but their results can differ. Here we used available colpodean ciliate OTUs from forest soils compare the assignments VSEARCH (which performs pairwise alignments) EPA‐ng phylogenetic placements). We showed that when there are differences...
Abstract Taxonomic assignment of OTUs is an important bioinformatics step in analyzing environmental sequencing data. Pairwise-alignment and phylogenetic-placement methods represent two alternative approaches to taxonomic assignments, but their results can differ. Here we used available colpodean ciliate from forest soils compare the assignments VSEARCH (which performs pairwise alignments) EPA-ng phylogenetic placements). We showed that when there are differences between alignments...