The process of cell death caused by influenza virus infection in cultured MDCK and HeLa cells was analysed. This gave rise to nuclear fragmentation chromatin condensation accompanied chromosomal DNA into oligonucleosomes. Chromosomal progressed concomitantly with lysis cells, producing high low yields particles, respectively, indicating that the extent not proportional production. endonuclease inhibitor zinc blocked cells. Cycloheximide inhibited as well lysis. Inhibition occurred when drug...

Influenza virus-infected cells undergo apoptosis and become susceptible to phagocytosis by macrophages in vitro, this leads the propagation of virus being inhibited. We previously showed that inhibitors increased rate mortality among influenza mice. However, mode vivo has not been investigated. We, study, assessed issue histochemically analyzing bronchoalveolar lavage lung tissue obtained from C57BL/6 mice infected with A/WSN (H1N1) virus. Both neutrophils accumulated soon after viral...

The mechanism of phagocytic elimination dying cells in Drosophila is poorly understood. This study was undertaken to examine the recognition and engulfment apoptotic by hemocytes/macrophages vitro vivo. In analysis, l(2)mbn (a cell line established from larval hemocytes a tumorous mutant) were used as phagocytes. When treated with molting hormone 20-hydroxyecdysone, acquired ability phagocytose S2 cells, another line. undergoing cycloheximide-induced apoptosis exposed phosphatidylserine on...

Some synthetic lipopeptides, in addition to native lipoproteins derived from both Gram-negative bacteria and mycoplasmas, are known activate TLR2 (Toll-like receptor 2). However, the inherent Gram-positive bacteria, which function as ligands, have not been characterized. Here, we purified a lipoprotein homogeneity Staphylococcus aureus study ligand. The 33-kDa was capable of stimulating identified triacylated SitC lipoprotein, belongs family ATP binding cluster (ABC) transporter...

Although ischemia-reperfusion of mouse kidney is known to cause severe renal failure due tubular cell death, the exact cellular mechanism responsible for this phenomenon not clear. To investigate spatial and temporal development death role Fas/APO-1/CD95 (Fas) in process, left vessels were occluded a group mice 30, 60, or 120 min followed by reperfusion 24 h (n = 4 each group). Analysis isolated DNA agarose-gel electrophoresis revealed typical ladder pattern bands consisting multiples 180...

Previously we reported that ischemia results in apoptosis and is accompanied by phosphorylation on Tyr-701 increased expression transcriptional activity of the signal transducer activator transcription-1 (STAT-1). In present study, show exposure cardiomyocytes to induced STAT-1 at another site, Ser-727. Moreover, critical for induction Fas receptor ligand ischemia/reperfusion (I/R). Transcriptional activation FasL was dependent Ser-727 but independent Tyr-701. Similarly, not required...

Rat Sertoli cells phagocytose apoptotic spermatogenic cells, which consist mostly of spermatocytes, in primary culture by recognizing phosphatidylserine (PS) exposed on the surface degenerating cells. We compared mode phagocytosis using at different stages spermatogenesis. Spermatogenic were separated into several groups based their ploidy, with purities 60–90%. When fractionated cell populations subjected to a assay, ploidies 1<i>n</i>, 2<i>n</i>, and 4<i>n</i> almost equally phagocytosed...

ABSTRACT Some cultured cell lines undergo typical apoptosis upon infection with influenza virus. However, the release of replicated virus into culture medium does not change when is inhibited. Since apoptotic cells are heterophagically eliminated at early stages pathway, we anticipated that coexistence phagocytic virus-infected affects extent growth. When A HeLa were mixed activated mouse peritoneal macrophages, efficient phagocytosis, which was abrogated in presence a caspase inhibitor,...

We reported previously that influenza virus infection induces the apoptotic death of HeLa cells associated with activation Fas gene. In this report, we show transfection a PKR having point mutation in catalytic domain K at 296 to R suppressed both augmented expression and cell by infection. These results suggested involvement virus-induced death.

In a primary co-culture of spermatogenic and Sertoli cells the rat, many die by apoptosis are subsequently engulfed cells. We investigated mechanism this phagocytosis reaction. Testicular from 20-day-old rats were cultured, separated. When recovered maintained without cells, viability decreased they became more susceptible to Phagocytosis was severely impaired when liposomes containing acidic phospholipids, such as phosphatidylserine, phosphatidylinositol, cardiolipin, included in reaction,...

The high power ability of humanoid is desired for application nursing or running jumping motions. Achievement the actuator light and powerful equivalent to humans required. In this paper, we propose a method extract inherent performance from motors by an active temperature control. safely improves output motors. control achieved combining estimation internal motor with forced cooling liquid. We also developed drivers proposed method. An experiment joint test bench shown. show prototype biped...

We investigated whether phagocytosis participates in the protection of insects from viral infection using natural host-virus interaction between Drosophila melanogaster and C virus (DCV). S2 cells were induced to undergo apoptotic cell death upon DCV infection. However, UV-inactivated was unable cause apoptosis, indicating need for productive apoptosis induction. became susceptible by hemocyte-derived l(2)mbn after infection, presence phagocytes cultures reduced proliferation. Phagocytosis...

Journal Article Phosphatidylserine recognition and induction of apoptotic cell clearance by Drosophila engulfment receptor Draper Get access Tran Thanh Tung, Tung 1Graduate School Natural Science Technology, Kanazawa University, Kanazawa, Ishikawa 920-1192, Japan; 2Department Pharmacology, Hanoi Medical No 1. Ton That Hanoi, Socialist Republic Vietnam; 3Graduate Sciences; 4School Pharmacy, Japan Search for other works this author on: Oxford Academic PubMed Google Scholar Kaz Nagaosa, Nagaosa...

Apoptosis of germ cells is very common in normal and injured mammalian testes. The aim this study was to examine the possible involvement Fas ligand (FasL) system induction cell apoptosis ischemia-reperfusion testes adult mice. assessed by TUNEL method DNA gel electrophoresis. FasL mRNAs were detected Northern blotting reverse transcription polymerase chain reaction techniques, proteins analyzed Western immunohistochemistry. identified testis especially around stages XI XII, whereas...

Abstract Toll-like receptor 4 (TLR4) of macrophages recognizes LPS Gram-negative bacteria in cooperation with CD14, which is also involved the recognition apoptotic cells. In this study we asked whether TLR4 plays a role phagocytic clearance cells by macrophages. Macrophages were prepared from peritoneal fluid thioglycolate-treated mice carrying either wild-type or disrupted TLR4-encoding gene and examined for their ability to phagocytose mouse thymocytes, Jurkat T cells, Ig-opsonized...

Testicular Sertoli cells phagocytose apoptotic spermatogenic in a manner depending on the membrane phospholipid phosphatidylserine (PS) expressed at surface of latter cell type. Our previous studies have indicated that class B scavenger receptor type I (SR-BI) is responsible for PS-mediated phagocytosis by cells. We examined here whether SR-BI binds directly to PS. A line acquired ability bind PS-exposing and incorporate PS-containing liposomes when it was forced express SR-BI. Furthermore,...

Abstract TLR2 plays a role as pattern-recognition receptor in the innate immune response involving secreted proteins against microbial pathogens. To examine its possible involvement cellular response, we determined levels of engulfment and subsequent killing bacteria by macrophages prepared from TLR2-deficient wild-type mice. The level Staphylococcus aureus or Escherichia coli was almost same between TLR2-lacking macrophages. However, colony-forming ability engulfed S. aureus, but not E....

Recent findings on the translocation of intact fibroblast growth factor (FGF) into cell nucleus suggest that it functions directly in nuclear events. We examined effect human basic FGF (bFGF) gene transcription a cell-free system. When mouse genes encoding phosphoglycerate kinases 1 and 2 (Pgk-1 Pgk-2) were transcribed by using extracts Ehrlich ascites tumor cells, affected different ways: presence bFGF, Pgk-1 was inhibited, whereas Pgk-2 enhanced. viral tested, adenovirus major late DNA...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification and preparation of antibody to RNA polymerase II stimulatory factors from Ehrlich ascites tumor cellsKazuhisa Sekimizu, Yoshinobu Nakanishi, Den'ichi Mizuno, Shunji NatoriCite this: Biochemistry 1979, 18, 8, 1582–1588Publication Date (Print):April 17, 1979Publication History Published online1 May 2002Published inissue 17 April 1979https://pubs.acs.org/doi/10.1021/bi00575a031https://doi.org/10.1021/bi00575a031research-articleACS...

Phagocytosis is central to cellular immunity against bacterial infections. As in mammals, both opsonin-dependent and -independent mechanisms of phagocytosis seemingly exist Drosophila. Although candidate Drosophila receptors for have been reported, how they recognize bacteria, either directly or indirectly, remains be elucidated. We searched the Staphylococcus aureus genes required by hemocytes a screening mutant strains with defects structure cell wall. The identified included ltaS, which...