A5065071678- Neurobiology and Insect Physiology Research
- Insect Utilization and Effects
- Insect and Arachnid Ecology and Behavior
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Invertebrate Immune Response Mechanisms
- Physiological and biochemical adaptations
- Insect Pest Control Strategies
- Neuropeptides and Animal Physiology
- Antimicrobial Peptides and Activities
- Genetics, Aging, and Longevity in Model Organisms
- Insect behavior and control techniques
- Protein Hydrolysis and Bioactive Peptides
- Animal Behavior and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Plant and animal studies
- Receptor Mechanisms and Signaling
- Neuroendocrine regulation and behavior
- Advanced Proteomics Techniques and Applications
- Entomological Studies and Ecology
- Origins and Evolution of Life
- Phytochemical compounds biological activities
- Insects and Parasite Interactions
- Photoreceptor and optogenetics research
- Crustacean biology and ecology
KU Leuven
2014-2023
Vlaams Instituut voor Biotechnologie
1997-2010
UCLouvain
1982-2004
Zoological Institute
1982-2003
Bangladesh Atomic Energy Commission
2003
Southern Plains Agricultural Research Center
2002
University of Michigan
2002
Agricultural Research Service
2002
Catholic University of America
2001
Hebrew University of Jerusalem
1999
Neuropeptides regulate most, if not all, biological processes in the animal kingdom, but only seven have been isolated and sequenced from Drosophila melanogaster. In analogy with proteomics technology, where all proteins expressed a cell or tissue are analyzed, peptidomics approach aims at simultaneous identification of whole peptidome tissue,i.e. peptides their posttranslational modifications. Using nanoscale liquid chromatography combined tandem mass spectrometry data base mining, we...
In response to crowding, locusts develop characteristic black patterns that are well discernible in the gregarious phase at outbreaks. We report here a dark-color-inducing neuropeptide (dark-pigmentotropin) from corpora cardiaca of two plague locusts, Schistocerca gregaria and Locusta migratoria. The chromatographic isolation this was monitored by using bioassay with an albino mutant L. neurohormone, consisting 11 amino acids, is identical [His7] corazonin, previously isolated another...
In the classical "mind-body" wording, "body" is usually associated with "mass aspect" of living entities and "mind" "immaterial" one. Thoughts, consciousness soul are classified as immaterial. A most challenging question emerges: Can something that truly immaterial, thus in wording physics has no mass, exist at all? Many will answer: "No, impossible." My answer it very well possible, esoteric mechanisms need to be invoked, but this possibility inherent 2 established undervalued physiological...
Abstract Peptides are the largest class of signalling molecules found in animals. Nevertheless, most proteomic studies peptides overlooked since they literally fall through mazes net. In analogy with proteomics technology, where all proteins expressed a cell or tissue analyzed, peptidomic approach aims at simultaneous visualization and identification whole peptidome tissue, i.e. their post‐translational modifications. this paper we describe analysis larval fruit fly central nervous system...
Insects respond to microbial infection by the rapid and transient expression of several genes encoding antibacterial peptides. In this paper we describe a powerful technique, two-dimensional difference gel electrophoresis, that, when combined with mass spectrometry, can be used study immune response Drosophila melanogaster at protein level. By comparatively analyzing hemolymph proteome 2,000 third-instar larvae, identified 10 differential proteins that appear in fruit fly very early after an...
The pars intercerebralis–corpora cardiaca system (PI–CC) of insects is the endocrinological equivalent hypothalamus–pituitary vertebrates. Peptide profiles intercerebralis and corpora were characterized using simple sampling protocols in combination with MALDI‐TOF electrospray ionization double quadrupole time flight (ESI‐Qq‐TOF) mass spectrometric technologies. results compared earlier conventional sequencing methods immunocytochemical methods. In addition to many known peptides, several m...
The cDNA for Tyr-Loc, a G protein-coupled receptor that clearly shows homology to number of mammalian and fruit fly receptors biogenic amines, was cloned from the nervous system Locusta migratoria. Functional expression obtained in cultured insect cells, i.e., Spodoptera SF9 cells using baculoviral stably transformed Drosophila Schneider 2 (S2) cells. Multiple copies construct are inserted into genome these permanently driven by upstream sequences Bombyx mori immediate early gene. Tyramine...
Background The desert locust (Schistocerca gregaria) displays a fascinating type of phenotypic plasticity, designated as 'phase polyphenism'. Depending on environmental conditions, one genome can be translated into two highly divergent phenotypes, termed the solitarious and gregarious (swarming) phase. Although many underlying molecular events remain elusive, central nervous system (CNS) is expected to play crucial role in phase transition process. Locusts have also proven interesting model...