A5071351889- Photosynthetic Processes and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Enzyme Structure and Function
- Plant biochemistry and biosynthesis
Okayama University of Science
2022-2024
Okayama University
2019-2021
University of Hyogo
2019-2021
Photosynthetic water oxidation is catalyzed by the Mn4CaO5 cluster of photosystem II (PSII) with linear progression through five S-state intermediates (S0 to S4). To reveal mechanism oxidation, we analyzed structures PSII in S1, S2, and S3 states x-ray free-electron laser serial crystallography. No insertion was found but flipping D1 Glu189 upon transition leads opening a channel provides space for incorporation an additional oxygen ligand, resulting open cubane Mn4CaO6 oxyl/oxo bridge....
Abstract Photosystem II (PSII) catalyses the oxidation of water through a four-step cycle S i states ( = 0–4) at Mn 4 CaO 5 cluster 1–3 , during which an extra oxygen (O6) is incorporated 3 state to form possible dioxygen 4–7 . Structural changes metal and its environment S-state transitions have been studied on microsecond timescale. Here we use pump-probe serial femtosecond crystallography reveal structural dynamics PSII from nanoseconds milliseconds after illumination with one flash (1F)...
Photosystem II (PSII) catalyzes light-induced water oxidation through an S i -state cycle, leading to the generation of di-oxygen, protons and electrons. Pump–probe time-resolved serial femtosecond crystallography (TR-SFX) has been used capture structural dynamics light-sensitive proteins. In this approach, it is crucial avoid light contamination in samples when analyzing a particular reaction intermediate. Here, method for determining condition that avoids PSII microcrystals while...
Abstract Photosystem II (PSII) catalyzes light-induced water oxidation through an Si-state cycle, leading to the generation of di-oxygen, protons, and electrons. Pump-probe time-resolved serial femtosecond crystallography (TR-SFX) has been used capture intermediate states light-driven enzymatic reactions. In this approach, it is crucial avoid contamination light into samples when analyzing a particular reaction intermediate. Here, we describe method for determining proper condition that...