A5065673016- Vestibular and auditory disorders
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Malaria Research and Control
- Hearing, Cochlea, Tinnitus, Genetics
- Voice and Speech Disorders
- Ear Surgery and Otitis Media
- Enzyme Structure and Function
- Head and Neck Surgical Oncology
- Pancreatic and Hepatic Oncology Research
- Ear and Head Tumors
- Mosquito-borne diseases and control
- Head and Neck Cancer Studies
- Tracheal and airway disorders
- Ophthalmology and Eye Disorders
- Dysphagia Assessment and Management
- Chemical Synthesis and Analysis
- Salivary Gland Tumors Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Synthesis and Characterization of Heterocyclic Compounds
- Crystallography and molecular interactions
- Hearing Loss and Rehabilitation
- Pancreatitis Pathology and Treatment
- Glaucoma and retinal disorders
- Head and Neck Anomalies
Osaka University
2016-2025
Protein Research Foundation
2012-2024
Tokyo Medical University
2012-2023
Japan Synchrotron Radiation Research Institute
1997-2022
University of Tsukuba
1997-2019
Health Services Research & Development
2019
Iwate University
2018-2019
Toyota Industries (United States)
2018
SPring-8
2001-2017
RIKEN
1993-2017
This paper presents diagnostic criteria for Menière's disease jointly formulated by the Classification Committee of Bárány Society, The Japan Society Equilibrium Research, European Academy Otology and Neurotology (EAONO), American Otolaryngology-Head Neck Surgery (AAO-HNS) Korean Balance Society. classification includes two categories: definite probable disease. diagnosis is based on clinical requires observation an episodic vertigo syndrome associated with low- to medium-frequency...
Abstract Photosystem II (PSII) catalyses the oxidation of water through a four-step cycle S i states ( = 0–4) at Mn 4 CaO 5 cluster 1–3 , during which an extra oxygen (O6) is incorporated 3 state to form possible dioxygen 4–7 . Structural changes metal and its environment S-state transitions have been studied on microsecond timescale. Here we use pump-probe serial femtosecond crystallography reveal structural dynamics PSII from nanoseconds milliseconds after illumination with one flash (1F)...
The mammalian endoplasmic reticulum (ER) harbors disulfide bond-generating enzymes, including Ero1α and peroxiredoxin 4 (Prx4) nearly 20 members of the protein isomerase family (PDIs), which together constitute a suitable environment for oxidative folding. Here, we clarified Prx4 preferential recognition two PDI proteins, P5 ERp46 mode interaction between thioredoxin domain. Detailed analyses folding catalyzed by reconstituted Prx4–PDIs pathways demonstrated that, while are dedicated to...
Significance Copper nitrite reductase (CuNiR) is involved in denitrification of the nitrogen cycle. Synchrotron X-rays rapidly reduce copper sites and decompose substrate complex structure, which has made crystallographic studies CuNiR difficult. Using femtosecond X-ray free electron lasers, we determined intact structures with without nitrite. Based on obtained structures, proposed a redox-coupled proton switch model, provides an explanation for proton-coupled transfer (PCET) CuNiR. PCET...
An algorithm for the protection of power transformers is described in which are represented by a universal equivalent circuit composed inverse inductance, without reference to an internal fault. The derived from multiterminal network analysis windings. intrinsic characteristics elements clarified calculations actual transformers. inductance obtained sampling voltage and current at each terminal used discriminate between faults magnetizing inrush. Computer simulations large experimental...
We used directed evolution to enhance the thermostability of glycosyl hydrolase family-11 xylanase from <i>Bacillus subtilis</i>. By combining random point mutagenesis, saturation and DNA shuffling, a thermostable variant, Xyl<sup>st</sup>, was identified which contained three amino acid substitutions: Q7H, N8F, S179C. The half-inactivation temperature (the midpoint melting curves) for Xyl<sup>st</sup> variant compared with wild-type enzyme after incubation 10 min elevated 58 68 °C. At 60 °C...
An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and two-dimensional detector. The chamber the are operated under helium atmosphere at 1 atm. ambient pressure operation facilitates applications to samples. Three kinds employed feed randomly oriented crystals in aqueous solution or highly viscous fluid. Experiments on lysozyme were performed by 10 keV XFEL...
Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading low flow rate that enables the determination structure, while requiring consumption less than 1 mg sample. However, reliable and versatile wide variety protein samples is still elusive. Here we introduce hydroxyethyl cellulose-matrix carrier, determine structure three proteins....
Significance The influenza M2 channel is among the smallest proton-selective channels found in nature. High-resolution structures of this further our understanding how protons are transported across a membrane bilayer through constricted protein environment. This study also has broader implications for structural interpretation water using X-ray crystallographic techniques. Previous structure determined synchrotron radiation with cryocooling were biased by low temperature, and room...
The skills of ear surgery are best developed by dissecting a temporal bone. However, only limited number trainees can be afforded this opportunity because the scarcity available bones. aim study was to investigate validity prototype bone model for surgical training and education.A simulated 3D human made using selective laser sintering method. powder layers were laser-fused based on detailed CT data accumulated create structure. Conventional instruments used dissect under microscope.The as...
A recently developed predictive out-of-step protection system is described. The purpose of this to prevent total collapse caused by step-out between large-capacity generator groups due a serious fault in the trunk transmission line. This accomplished using online data collected during incubation phase predict and then issuing system-seperation command. has following characteristics: (1) difference several large measured from voltage substation busbars vicinity generators at sampling rate 600...