A5032489820- Cardiovascular Conditions and Treatments
- Congenital Heart Disease Studies
- Pulmonary Hypertension Research and Treatments
- Cardiovascular Function and Risk Factors
- Mesenchymal stem cell research
- Sphingolipid Metabolism and Signaling
- Cardiovascular Effects of Exercise
- Cardiac Valve Diseases and Treatments
- Cardiomyopathy and Myosin Studies
- Cardiac Arrhythmias and Treatments
- Carcinogens and Genotoxicity Assessment
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Veterinary Oncology Research
- Lipid Membrane Structure and Behavior
- Lipid metabolism and biosynthesis
- Medical and Health Sciences Research
- Nitric Oxide and Endothelin Effects
- Estrogen and related hormone effects
- Gut microbiota and health
- Gastrointestinal motility and disorders
- Coronary Artery Anomalies
- Veterinary Medicine and Surgery
- Immune cells in cancer
- Clostridium difficile and Clostridium perfringens research
Nippon Veterinary and Life Science University
2016-2025
Yamagata University
2022-2024
Nagasaki University
2023
Suzuki (Japan)
2022
University of Veterinary Medicine
2022
Tohoku University
2014-2021
Hiratsuka Kyosai Hospital
2017
Hokkaido University
2009-2016
BioChem Technology (United States)
2015
Japan Science and Technology Agency
2014
Ovarian cancer shows high mortality due to development of resistance chemotherapy and relapse. Cancer stem cells (CSCs) have been suggested be a major contributor in developing drug relapse ovarian cancer. In this study, we isolated CSCs through sphere culture A2780, SKOV3, OVCAR3 epithelial primary from patients. We identified heat-stable factors secreted stimulated migration proliferation CSCs. Mass spectrometry ELISA analysis revealed that lysophosphatidic acid (LPA) was significantly...
Lysophosphatidic acids (LPA) have important roles in the field of vascular biology and are derived mainly from lysophosphatidylcholine via autotaxin. However, our previous study, only plasma LPA levels, not serum autotaxin increased patients with acute coronary syndrome (ACS). The aim this study was to elucidate pathway by which is ACS.We measured lysophospholipids species 141 consecutive undergoing angiography (ACS, n=38; stable angina pectoris, n=71; angiographically normal arteries, n=32)...
Three new communesin congeners, communesins C, D, and E, together with two known A B were isolated from okara that had been fermented Penicillium expansum Link MK-57. All these showed the insecticidal activity against silkworms.
Lysophosphatidic acid (LPA) is a blood-derived bioactive lipid with numerous biological activities exerted mainly through six defined G protein-coupled receptors (LPA1-LPA6). LPA was first identified as vasoactive compound because it induced transient hypertension when injected intravenously in rodents. Here, we examined the molecular mechanism underlying LPA-induced hypertensive response. The response significantly attenuated by pretreatment Rho kinase inhibitor, which blocks Gα12/13...
Recently, fecal microbiota transplantation (FMT) has been tested in veterinary medicine as a treatment option for multiple gastrointestinal (GI) diseases, such inflammatory bowel disease (IBD). However, there are no reports of changes the microbial diversity microbiome after with FMT canine IBD cases. Moreover, little is known about long-term efficacy and safety dogs. Herein, we present case intractable treated repeated, FMT.The patient was 10-year-old, neutered, male, 4-kg Toy Poodle...
In human medicine, fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridioides difficile infection. It has also been tested as a multiple gastrointestinal diseases, including inflammatory bowel disease (IBD). However, only few studies have focused on the changes in microbiome following FMT canine IBD. Here, we performed nine dogs with IBD using matter of healthy and investigated subsequent clinical signs. three dogs, was examined by 16S rRNA sequencing....
Lysophosphatidic acids (LysoPAs) and lysophosphatidylserine (LysoPS) are emerging lipid mediators proposed to be involved in the pathogenesis of acute coronary syndrome (ACS). In this study, we attempted elucidate how LysoPA LysoPS become elevated ACS using human blood samples collected simultaneously from culprit arteries peripheral subjects. We found that: 1) plasma LysoPA, LysoPS, lysophosphatidylglycerol levels were not different, while lysophosphatidylcholine (LysoPC),...
Lysophosphatidic acid (LPA) is a potent signaling lipid, and state-dependent alterations in plasma LPA make it promising diagnostic marker for various diseases. However, concentrations vary widely among reports, even under normal conditions. These variations can be attributed, at least part, to the artificial metabolism of after blood collection. Here, we aimed develop an optimized preparation method that reflects concentration circulating blood. The main features devised were suppression...
This study aimed to investigate the effects of oxidative stress and antioxidant activity in plasma uterine fluid during early postpartum on reproductive performance Japanese Black cows. A total 17 cows were included this study. Plasma samples collected at days 7 14 estrus cycle (n = 17), between 47–67 postpartum, diacron-reactive oxygen metabolites (d-ROMs) biological potential (BAP) measured. d-ROMs, BAP, oxidation index (OSI: d-ROMs/BAP × 100) compared pregnant nonpregnant first artificial...
Hypertrophic cardiomyopathy (HCM) is the most prevalent cardiac disease in cats, and one phenotype includes obstructive HCM with dynamic left ventricular outflow tract obstruction (DLVOTO). Myocardial function has been reported to be lower cats than non-obstructive HCM. Carvedilol, because of its pharmacological action, expected reduce pressure gradient associated DLVOTO, but no previous reports have studied effects on myocardial function. This study aimed evaluate treated by carvedilol...
Hypertrophic cardiomyopathy (HCM) is the most common cardiac disease in cats. HCM classified into obstructive (HOCM) and non-obstructive (HNCM) forms based on presence or absence of left ventricular outflow tract obstruction (LVOTO). In humans, LVOTO a known poor prognostic factor target for therapeutic intervention. contrast, has been reported not to contribute prognosis This study demonstrated significant correlations between cTnI levels maximal LV velocity (LVOTV). finding suggests that...
Abstract Objective —To investigate the roles of transforming growth factor-β (TGF-β) isoforms and matrix metalloproteinases (MMPs) in development chronic mitral valvular disease (CMVD) dogs. Sample Population —12 valve leaflets collected from cadavers 5 clinically normal dogs 7 with CMVD. Procedures —Expression TGF-β 1, 2, 3; MMPs 3, 9; receptor II (TβR-II); α smooth muscle actin (αSMA) valves CMVD was compared that Additionally, responses interstitial cells (VICs) to TGF-β3, MMP-3,...
Background Left ventricular torsional motion plays an important role for effective pump function. However, noninvasive clinical assessment of deformations by two‐dimensional speckle‐tracking echocardiography (2D‐ STE ) in dogs with myxomatous mitral valve disease ( MMVD has not been reported. Hypothesis torsion is determined the native orientation helical myocardial fibers, such that it might provide better function than conventional methods. Animals Sixty‐seven client‐owned were classified...
Glycero-lysophospholipids (glycero-LPLs), which are known to exert potent biological activities, have been demonstrated be secreted from activated platelets in vitro; however, their association with platelet activation vivo has not yet elucidated. In this study, we investigated the correlations between blood levels of each glycero-LPL and serotonin, a biomarker activation, human subjects elucidate involvement glycero-LPLs vivo.We measured plasma serotonin 141 consecutive patients undergoing...
Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are an attractive source for cell-based therapy of some diseases, including acute and chronic liver failure, in not only human medicine but also veterinary medicine. However, medicine, no studies have reported the effects AT-MSCs on injury dogs. The purpose this study was to investigate allogenic by carbon tetrachloride dogs compare therapeutic transplanted via peripheral vein (PV) or splenic (SV). After transplantation through PV SV,...
Abstract Background Progression to combined post‐ and pre‐capillary pulmonary hypertension (PH) provides prognostic information in human patients with post‐capillary PH. Pulmonary vascular resistance estimated by echocardiography (PVRecho) is useful for the stratification of dogs myxomatous mitral valve disease (MMVD) detectable tricuspid regurgitation. Objectives To evaluate value PVRecho MMVD. Animals Fifty‐four MMVD Methods Prospective cohort study. All underwent echocardiography. The was...