A5000780291- Veterinary Oncology Research
- Mesenchymal stem cell research
- Infectious Diseases and Mycology
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Virus-based gene therapy research
- Immune cells in cancer
- Hedgehog Signaling Pathway Studies
- Peptidase Inhibition and Analysis
- Melanoma and MAPK Pathways
- Sarcoma Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Complement system in diseases
- Soft tissue tumors and treatment
- Platelet Disorders and Treatments
- Cutaneous Melanoma Detection and Management
- Mast cells and histamine
- Urologic and reproductive health conditions
- Genital Health and Disease
- Prostate Cancer Diagnosis and Treatment
- Hypertrophic osteoarthropathy and related conditions
- Galectins and Cancer Biology
- RNA modifications and cancer
- Lymphoma Diagnosis and Treatment
Nippon Veterinary and Life Science University
2017-2025
Shinshu University Hospital
2024
Yamaguchi University
2024
Veterinary Medical Center
2022
In cancer cells, folate is metabolised in the cytoplasm and mitochondria. Folate metabolism mediates nucleic acid synthesis and, thereby, growth of cells. One enzymes within this metabolic pathway, methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2 (MTHFD2), rarely expressed normal adult cells but abundantly mitochondria nuclei foetal humans mice. study, we examined localisation function MTHFD2 canine mammary was carcinoma cell lines. Short-term inhibition with an inhibitor reduced...
Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are an attractive source for cell-based therapy of some diseases, including acute and chronic liver failure, in not only human medicine but also veterinary medicine. However, medicine, no studies have reported the effects AT-MSCs on injury dogs. The purpose this study was to investigate allogenic by carbon tetrachloride dogs compare therapeutic transplanted via peripheral vein (PV) or splenic (SV). After transplantation through PV SV,...
Mesenchymal stem cells are expected to be a cell source for therapy of various diseases in veterinary medicine. However, donor-dependent heterogenicity has been cause inconsistent therapeutic efficiency. Therefore, we established immortalized from canine adipose tissue-derived mesenchymal (ADSCs) minimize cellular heterogeneity by reducing the number donors, evaluated their properties, and compared them primary with RNA-sequencing. Immortalized ADSCs were transduction combinations R24C...
Mammary adenocarcinoma, the most common cancer in female dogs, often exhibits lymph node and lung metastases has a higher mortality rate. However, mammary adenocarcinoma no established treatment, except early surgical excision. Canine carcinoma many features with human carcinoma, including clinical characteristics, heterogeneity, genetic aberrations, making it an excellent spontaneous tumor model for breast cancer. Diverse cancers comprised heterogeneous cell populations originating from...
The potential of mesenchymal stem cells (MSCs) to differentiate into nonmesodermal such as pancreatic beta has been reported. New cell-based therapy using MSCs for diabetes mellitus is anticipated an alternative treatment option insulin injection or islet transplantation in both human and veterinary medicine. Several protocols were reported differentiation insulin-producing (IPCs), but no studies have IPCs generated from canine MSCs. purpose this study was generate adipose tissue-derived...
Abstract Canine prostate cancer (cPCa) is a malignant neoplasm with no effective therapy. The BRAF V595E mutation, corresponding to the human V600E found frequently in cPCa. Activating mutations are recognized as oncogenic drivers, and blockade of MAPK/ERK phosphorylation may be an therapeutic target against BRAF‐mutated tumours. aim this study was establish novel cPCa cell line clarify antitumor effects MEK inhibitors on vitro vivo. We established CHP‐2 that derived from prostatic tissue...
Abstract Background Canine malignant melanoma is highly aggressive and generally chemoresistant. Toceranib a kinase inhibitor drug that inhibits several tyrosine kinases including the proto-oncogene receptor KIT. Although canine cells often express KIT, therapeutic effect for toceranib has yet to be reported this tumor, with only small number of patients studied date. This case report dog experienced transient response toceranib. Furthermore, KIT expressed in tumor was examined using...
Mesenchymal stem cells (MSCs) are a promising cell source for therapy of intractable diseases in veterinary medicine, but donor-dependent cellular heterogeneity is an issue that influences therapeutic efficacy. Thus, we previously established immortalized maintain the fundamental properties primary cells, functional evaluation had not been performed. Therefore, evaluated immunomodulatory capacity canine adipose-derived MSCs (cADSCs) vitro and vivo to investigate whether they functions....
Introduction The therapeutic efficacy of mesenchymal stromal cells (MSCs) in inflammatory bowel disease is not completely known and consistent. Priming with cytokines has been proposed to adapt MSCs an environment have them ready counteract it, but may undesirable effects on MSCs, such as increased immunogenicity. In this study, we hypothesized that priming inflamed intestinal tissue would more effectively enhance their effect inflammation. Methods capacity canine adipose-derived (cADSCs)...
Chondrosarcoma is the second most common primary bone tumor after osteosarcoma in dogs. has a good prognosis owing to its low metastatic rate and long survival time, even with amputation alone. However, risks reducing quality of life patients other orthopedic diseases non-affected limb, neurological diseases, or large body size. Limb-sparing surgery frozen autologous grafting using liquid nitrogen allows be maintained normal area while killing cells, thereby preserving affected limb. Thus,...
A 16-year-old male mixed-breed dog presented with a mass hemorrhage at the right conjunctiva. Five months after initial visit, eye protruded and had firm irregular measuring approximately 1.00 cm in diameter conjunctival hemorrhage. Microscopically, was comprised polygonal or round tumor cells distinct cell borders arranged nested diffuse pattern. The round-to-oval fine hyperchromatic nuclei containing multiple nucleoli abundant eosinophilic pale cytoplasm. Multiple giant were frequently...
Canine histiocytic sarcoma (HS) is an aggressive and highly metastatic neoplasm. Mutations in src homology 2 domain-containing phosphatase (SHP2; encoded by PTPN11), which recently have been identified canine HS tumour cells, could be attractive therapeutic targets for SHP099, allosteric inhibitor of SHP2. Here, molecular characteristics wild-type SHP2 four mutants (p.Ala72Gly, p.Glu76Gln, p.Glu76Ala p.Gly503Val), including one that was newly the present study, were investigated....
A 4-year-old castrated male domestic shorthair cat with a continuous cough was brought to private veterinary clinic for detailed examination. Radiography of the thoracic cavity revealed severe radiopaque region in caudal lobe right lung. At 108 days after initial visit, CT showed mass 27 × 23 18 mm that time, no abnormalities other organs except lung were detected on and peripheral blood biochemistry tests. The resected by lobectomy; it had white surface firm. Histopathologically,...