A5056819507- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Diet, Metabolism, and Disease
- Sugarcane Cultivation and Processing
- Infant Nutrition and Health
- Aquatic Ecosystems and Phytoplankton Dynamics
- Environmental Toxicology and Ecotoxicology
- biodegradable polymer synthesis and properties
- Plant biochemistry and biosynthesis
- Digestive system and related health
- Marine Toxins and Detection Methods
- Amino Acid Enzymes and Metabolism
- Fermentation and Sensory Analysis
- Protein Hydrolysis and Bioactive Peptides
- Proteins in Food Systems
Pontificia Universidad Católica de Valparaíso
2016-2024
University of Concepción
2018
The one-pot production of tagatose from lactose using recombinant l-arabinose isomerase (L-AI) Thermotoga maritima and commercial β-galactosidase (β-gal) Aspergillus oryzae was evaluated. β-gal L-AI ratio (R β-gal/L-AI), initial concentration temperature were chosen as study variables. Maximum values yield (YTag), specific productivity (πTag) sugars (RTag/Gal) obtained 23 %, 0.202 ± 0.03 mmol Tag/(g prot⋅h) 1, respectively. composition the product on a dry basis follows (g/g): 0.23 tagatose,...
Despite the advance of knowledge about factors and potential mechanisms triggering ichthyotoxicity in microalgae, these remain unclear or are controversial for several species (e.g. Heterosigma). Neither typical toxicity tests carried out with cell extracts nor direct exposure to harmful were proved suitable unravel mechanism harm. Ichthyotoxic show a complex effect on fish, which is mediated through various depending species. In this work, we present method study sub-lethal effects...
The aromatic expression of wines can be enhanced by the addition specific glycosidases, although their poor stability remains a limitation. Coimmobilization glycosidases as cross-linked enzyme aggregates (combi-CLEAs) offers simple solution yielding highly stable biocatalysts. Nevertheless, small particle size combi-CLEAs hinders recovery, preventing industrial application. Encapsulation in alginate beads and polyvinyl alcohol is proposed solution. Combi-CLEAS β-d-glucosidase...
Hybrid bioinorganic biocatalysts have received much attention due to their simple synthesis, high efficiency, and structural features that favor enzyme activity stability. The present work introduces a biomineralization strategy for the formation of hybrid nanocrystals from β-galactosidase. effects immobilization conditions were studied, identifying important effect metal ions pH on yield recovered activity. For deeper understanding process, an in silico study was carried out identify ion...
Native whey obtained during casein micelle microfiltration was used as a novel source to produce galacto-oligosaccharides (GOS). Since the presence of macromolecules and other interferers reduces biocatalyst performance, this work evaluated effect different ultrasound processing conditions on GOS synthesis using concentrated native whey. Ultrasonic intensities (UI) below 11 W/cm2 tended increase activity in enzyme from Aspergillus oryzae for several minutes but accelerated inactivation that...