A5042272947- Analytical Chemistry and Chromatography
- Chromatography in Natural Products
- Crystallization and Solubility Studies
- Protein purification and stability
- Microfluidic and Capillary Electrophoresis Applications
- Phase Equilibria and Thermodynamics
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Surfactants and Colloidal Systems
- Innovative Microfluidic and Catalytic Techniques Innovation
- Mass Spectrometry Techniques and Applications
- Chemical and Physical Properties in Aqueous Solutions
- Electrostatics and Colloid Interactions
- Microbial Metabolites in Food Biotechnology
- Field-Flow Fractionation Techniques
- Metabolism and Genetic Disorders
- Spam and Phishing Detection
- RNA Interference and Gene Delivery
- Drug Solubulity and Delivery Systems
- Analytical Methods in Pharmaceuticals
- Biopolymer Synthesis and Applications
- Natural product bioactivities and synthesis
- Molecular Biology Techniques and Applications
- Liquid Crystal Research Advancements
Karlstad University
2015-2025
Uppsala University
2018-2019
In recent decades, there has been a trend toward using larger biological molecules as new active pharmaceutical ingredients (APIs) instead of the classical small organic API molecules.More recently, this shifted from very large biomolecules intermediate-sized APIs, such oligonucleotide therapeutics.Because their fundamental role in gene regulation, therapeutic oligonucleotides can be directed against specific ribonucleic acid (RNA) targets, representing promising customized approach for...
This study presents a systematic investigation of factors influencing the chromatographic separation diastereomers phosphorothioated pentameric oligonucleotides as model solutes. Separation was carried out under ion-pairing conditions using an XBridge C18 column. For with single sulfur substitution, diastereomer selectivity found to increase decreasing carbon chain length tertiary alkylamine used ion-pair reagent. Using reagent high for diastereomers, triethylammonium, it increased decreased...
Oligonucleotide drugs represent an emerging area in the pharmaceutical industry. Solid-phase synthesis generates many structurally closely related impurities, making efficient separation systems for purification and analysis a key challenge during drug development. To increase fundamental understanding of important preparative step, mass-overloaded injections fully phosphorothioated 16mer, i.e., deoxythymidine oligonucleotide, were performed on C18 phenyl column. The narrowest elution...
This study presents an improved workflow to support the development of machine learning models predict oligonucleotide retention times, peak widths and thus resolutions, from larger datasets where manual processing is not feasible. We explored diverse forms, ranging native fully phosphorothioated, using three different gradient slopes. Both phosphorothioated oligonucleotides were separated, a chromatographic C18 system with tributylaminium ion as ion-pair reagent in eluent, resulting time...
Here it was investigated how oligonucleotide retention and selectivity factors are affected by electrostatic non-electrostatic interactions in ion pair chromatography. A framework derived describing depends on the potential generated ion-pair reagent concentration, co-solvent volume fraction, charge difference between analytes, temperature. Isocratic experiments verified that, separation problems concerning oligonucleotides of different charges, increases with increasing surface analyte...
Support vector regression models are created and used to predict the retention times of oligonucleotides separated using gradient ion-pair chromatography with high accuracy. The experimental dataset consisted fully phosphorothioated oligonucleotides. Two were trained validated two pseudo-orthogonal modes three slopes. results show that spread in time differs between modes, which indicated varying degree sequence dependent separation. Peak widths from calculated correlated guanine-cytosine...
We investigated and compared the robustness of supercritical fluid chromatography (SFC) separations peptide gramicidin, using either isocratic or gradient elution. This was done design experiments in a space co-solvent fraction, water mass fraction co-solvent, pressure, temperature. The density eluent (CO2-MeOH-H2O) experimentally determined Coriolis flow meter to calculate volumetric rate required by design. For both retention models, most important factor total co-solvent. Comparing...
Ion-pair chromatography is the de facto standard for separating oligonucleotides and related impurities, particularly analysis but also often small-scale purification. Currently, there limited understanding of quantitative modeling both analytical overloaded elution profiles obtained during gradient in ion-pair chromatography. Here we will investigate a recently introduced mode, so-called ion-pairing reagent separations oligonucleotides. The first part study demonstrates how electrostatic...
A combined experimental and theoretical study was performed to understand how the pore size of packing materials with pores 60–300 Å in affects separation 5–50-mer oligonucleotides. For this purpose, we developed a model which solutes were described as thin rods estimate accessible surface area solute function size. First, an analytical investigation conducted found that selectivity increased by factor 2.5 when separating 5- 15-mer oligonucleotides using 300 rather than 100 pores. We...
Evaluation and analysis of environmentally sustainable extraction betulin from birch bark with a focus on large-scale industrial feasibility.
The inverse method is a numerical for fast estimation of adsorption isotherm parameters directly from few overloaded elution profiles and it was recently extended to acquisition in gradient conditions. However, the cumbersome due complex models found elution. In this case, physicochemically correct have very long calculation times. aim study investigate possibility using less model, with fewer adjustable parameters, but preserved/acceptable predictive abilities. We that equal or better...
Abstract When a chromatographic separation process is numerically optimized, number of input parameters to the column model need be accurately determined, and accuracy will affect reliability predicted optimal operational conditions. Furthermore, numerical solution have similar impact. The holdup volume, injection profiles, selection an algorithm for solving were investigated. Errors in or error found lead prediction product fraction where set purity and/or yield requirements are not met.
The fundamental aspects to be considered during method transfer in supercritical fluid chromatography (SFC) are reviewed. review is limited mobile phases, stationary and operating conditions generally encountered current practice. First, the fundamentals of retention SFC will explored relation composition, co-solvent adsorption phase, pressure, temperature. Second, considerations regarding predictable discussed instrumentation, columns, shifts, robustness. This not intended comprehensive but...