A5003753800- Peripheral Artery Disease Management
- Diabetic Foot Ulcer Assessment and Management
- Angiogenesis and VEGF in Cancer
- Aortic aneurysm repair treatments
- Cell Adhesion Molecules Research
- Wound Healing and Treatments
- Venous Thromboembolism Diagnosis and Management
- Cellular Mechanics and Interactions
- Diagnosis and Treatment of Venous Diseases
- Protease and Inhibitor Mechanisms
- Abdominal vascular conditions and treatments
- Protein Kinase Regulation and GTPase Signaling
- Nitric Oxide and Endothelin Effects
- Vascular Procedures and Complications
- Coronary Interventions and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Cardiac, Anesthesia and Surgical Outcomes
- Aortic Disease and Treatment Approaches
- Blood Coagulation and Thrombosis Mechanisms
- Infectious Aortic and Vascular Conditions
- Central Venous Catheters and Hemodialysis
- Pressure Ulcer Prevention and Management
- Vascular anomalies and interventions
- Atherosclerosis and Cardiovascular Diseases
- Renal and Vascular Pathologies
Yale University
2015-2024
Yale New Haven Health System
2006-2024
RELX Group (United States)
2022
Faculty of 1000 (United States)
2022
Yale New Haven Hospital
2002-2019
VA Connecticut Healthcare System
2004-2018
University of New Haven
1993-2018
Laboratoire d’Imagerie Biomédicale
2018
University Medical Center Utrecht
2014-2016
Harvard University
1998-2016
In vivo, endothelial cells (EC) are subjected to hemodynamic forces which may influence the production of nitric oxide. This study was designed examine effect cyclic strain on expression oxide synthase (eNOS) in cultured bovine aortic EC. EC were grown flexible membranes deformation at 60 cycles/min with -5 or -20 kPa vacuum. results an average 6 and 10%, respectively, is transmitted attached cells. Northern blot analysis total cytosolic RNA demonstrated increase eNOS gene both regimens but...
We examined the effect of repetitive mechanical stretching on smooth muscle cell (SMC) collagen production. Porcine SMCs from passages 3 through 7 were seeded in 35-mm2 flexible-bottomed culture wells at a concentration 2 x 10(5) cells per well and allowed to attach for 24 hours. The experimental group was placed vacuum-operated stress-providing instrument that exerted an average elongation 25% maximum downward deflection plate bottom. stretched (nine day) subjected cyclic force regimen 10 s...
Shear stress, the tangential component of hemodynamic forces, plays an important role in endothelial remodeling. In this study, we investigated Rho family GTPases Cdc42 and shear stress–induced signal transduction cytoskeleton reorganization. Our results showed that stress induced translocation from cytosol to membrane. Although both were involved transcription factor AP-1 acting on 12-O-tetradecanoyl-13-phorbol-acetate–responsive element (TRE), only was sufficient activate AP-1/TRE....
The vascular wall is continuously subjected to pulsatile hydrostatic pressures. However, most studies of smooth muscle cells (SMC) are done under static tissue culture conditions. We have utilized a flexible-bottom plate and applied cyclic tensional deformation cultured SMC. Recent shown that endothelial (EC) proliferate but remain randomly oriented when force regimen 3 cycle/min up 24% elongation. current experiments were performed study the effects similar on Porcine SMC seeded at 200,000...
The objective of this study was to determine whether focal adhesion proteins pp125FAK (focal kinase) and paxillin are phosphorylated on tyrosine might play a role in the morphological change cell migration induced by strain. Bovine aortic endothelial cells (EC) were subjected 10% average strain at 60 cycles/min. Cyclic increased phosphorylation 30 min (3.4-fold) 4 h (5.9-fold) (2.0-fold). Confocal microscopy showed that, after 4-h exposure strain, EC began elongate F-actin, pp125FAK,...
Tubular absorption (T) of two cationic proteins, lysozyme (LZM) and cytochrome c (CYT c), anionic beta 2-microglobulin (beta 2M) 125I-labeled human growth hormone (hGH), was studied in the isolated perfused rat kidney. All four proteins are extensively filtered and, at low loads, almost completely absorbed by tubular epithelium. TLZM TCYT is a saturable process high capacity (Tm) apparent affinity. (Tm)LZM orders magnitude larger than (Tm)CYT c. LZM inhibited dose-dependent reversible...