Due to the lack of an effective therapeutic treatment flavivirus, dengue virus (DENV) nonstructural protein 1 (NS1) has been considered develop a vaccine owing its role in antibody-dependent enhancement (ADE). However, both NS1 and antibody have shown cross-reactivity host molecules stimulated anti-DENV antibody-mediated endothelial damage platelet dysfunction. To overcome pathogenic events reactogenicity, human monoclonal antibodies (HuMAbs) against DENV were generated from DENV-infected...

<title>Abstract</title> Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as global health threat. The emergence of ZIKV increased the incidence neonates born with microcephaly or other neurological syndromes. majority infections are mild asymptomatic; however, clinical diagnosis inaccurate. Moreover, Dengue cross-reacts antibodies, which creates problems for serological infections. assays often performed dismally in dengue-endemic areas because this phenomenon. In...

Serology remains a useful indirect method of diagnosing tropical diseases, especially in dengue infection. However, the current literature regarding cross-reactivity between SARS-CoV-2 and serology is limited revealed conflicting results. As means to uncover relevant serological insight involving antibody classes against cross-reactivity, anti-SARS-CoV-2 IgA, IgM, IgG ELISA, based on spike nucleocapsid proteins, were selected for fever-presenting disease patient investigation. The study was...

Hand, foot, and mouth disease (HFMD) is a contagious childhood illness annually affects millions of children aged less than 5 years across the Asia–Pacific region. HFMD transmission mainly occurs through direct contact (person-to-person) indirect with contaminated surfaces objects. Therefore, public health measures to reduce spread in kindergartens daycare centers are essential. Based on guidelines by Department Disease Control, school closure policy for outbreaks wherein every Thailand must...

Most cases of dengue virus infection are mild, but severe can be fatal. Therefore, identification factors associated with severity is essential to improve patient outcomes and reduce mortality. The objective this study was assess associations between nutritional status among Thai children adolescents. This retrospective cross-sectional based on the medical records 355 patients treated at Hospital for Tropical Disease (Bangkok, Thailand) from 2017 2019. Subjects were aged less than 18 years...

Human enterovirus causes various clinical manifestations in the form of rashes, febrile illness, flu-like uveitis, hand–foot–mouth disease (HFMD), herpangina, meningitis, and encephalitis. Enterovirus A71 coxsackievirus are significant epidemic HFMD worldwide, especially children aged from birth to five years old. The genotype variants causing epidemics have been reported increasingly worldwide last decade. We aim use simple robust molecular tools investigate human enteroviruses circulating...

To determine the seroprevalence and transmission dynamics of dengue virus (DENV), age-stratified longitudinal serological surveys were conducted in Bangphae district, Ratchaburi province, Thailand, for 3 years between April 2012 2015.The enrolled healthy children adults 1 55 years-of-age, a serosurvey six repeated bleeds same cohort individuals was every 8 months first 2 (M0, M8, M16) half year (M24, M30, M36) rest study period. All samples tested using in-house indirect sandwich IgG ELISA...

In 2016, Zika virus (ZIKV) infection was declared a public health emergency of international concern because the neurological consequences in babies born to infected people. Because mild and nonspecific symptoms, serological tests are essential epidemiological studies. However, cross-reactive antibodies between other Flaviviridae members may complicate interpretation results these tests. This study investigated seroprevalence ZIKV Samut Songkhram central Thailand which affected by outbreak...

This study focused on evaluating the fumigation scheme and identifying problems encountered during operation in Bangkok Metropolitan Administration area. Ten district health officers working different teams of dengue outbreak control programme around had participated an in-depth interview. Five predetermined themes, including (i) surveillance strategy, (ii) quality availability equipment, (iii) delays, (iv) human resources, (v) area coverage, other emerging themes were addressed interviews....

Zika virus (ZIKV) is the mosquito-transmitted that WHO declared a Public Health Emergency of International Concern in 2016 due to consequence microcephaly from infected pregnancies. The incidence infection has been unclear many countries because most people have nonspecific febrile illnesses. This study’s aim investigate symptomatic infections archived samples dengue cohort study children central Thailand 2006 2009. We performed NS1 immunoglobulin (Ig)G enzyme-linked immunosorbent assay...

The coat protein (CP) of Papaya ringspot virus (PRSV) was analyzed for presentation the antigenic peptide animal virus, Canine parvovirus (CPV), in Escherichia coli (E. coli). 45 nucleotides fragment coding 15-aa epitope CPV-VP2 either inserted into PRSV-cp gene at 5', 3' ends, both 5' and ends or substituted end PRSV cp gene. Each chimeric genes cloned pRSET B vector under control T7 promoter transformed E. coli. recombinant proteins expressed from different were purified intraperitoneally...

Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as global health threat. The rise in ZIKV infections driven an increased incidence of neonates born with microcephaly or other neurological malformations. Therefore, screening for infection can considerably impact pregnant women, especially during the first trimester. majority are mild asymptomatic, and clinical diagnosis inaccurate. Moreover, given high level cross-reactivity among flaviviruses, serological...

&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Previous studies showed that ceftriaxone can cause acute kidney injury (AKI) in the pediatric population. This study proposed a cellular model of crystalline nephropathy ceftriaxone-associated AKI and explored related pathophysiology by using proteomic approach. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Ceftriaxone was crystallized with calcium artificial urine. Madin-Darby Canine Kidney (MDCK) cells, distal renal tubular cell, were...

The frequency of Zika virus (ZIKV)-specific IgA and IgM the cytokine expression profile ZIKV-infected patients in hyperendemic areas remain unclear. This study investigated rates ZIKV non-structural protein 1 (NS1)-specific evaluated serum levels Dengue (DENV) cases Thailand to identify potential diagnostic biomarkers, elucidate immunity against DENV, investigate association between symptoms. Low positivity for NS1-specific were detected our study. NS1 IgA/M (11%, 11/101) combination was...

Abstract Background Undernutrition has been shown to be associated with various infectious diseases. However, the recent improvement in nutritional status and management for diseases worldwide necessitates re-evaluation of this association. Methods A retrospective study was conducted children aged &amp;lt;14 years old dengue, malaria or acute diarrhea who visited were admitted Tha Song Yang hospital, near Thai–Myanmar border. Results Most patients had mild disease most undernourishment mild....

Dengue is one of the most serious mosquito-borne viral diseases occurring in humans. To combat complexity 4 antigenically distinct serotypes, ideal vaccine for dengue should be able to stimulate cross-neutralizing antibodies. Recently, genetics-based immune responses have been studied guide design against several pathogens. Despite a recent approval vaccine, information on virus (DENV) still limited. Consequently, we aimed determine profiles immunoglobulin heavy chain genes from DENV2...

Circulating hsa-miRNA-126 (CmiR-126) has been reported to involve in the pathogenesis of many infectious diseases including dengue virus infection. However, no prior study conducted describe more details dengue-infected pediatric patients. This aimed CmiR-126-3p patients during febrile and convalescent phases. Additionally, correlations between other relevant clinical laboratory factors were investigated. Sixty paired-serum specimens collected phases retrieved from with fever (DF) (n = 30)...

Arboviruses, particularly dengue virus (DENV), Zika (ZIKV), and Chikungunya (CHIKV), pose a growing threat to global public health. For disease burden estimation control, seroprevalence studies are paramount. This study was performed determine the prevalence of DENV, ZIKV, CHIKV on healthy individuals aged from 1-55 years old in Bangphae district, Ratchaburi province, Thailand. Enzyme-linked immunosorbent assays (ELISAs) rapid diagnostic tests (RDTs) were archived samples serological survey...

Hand, foot, and mouth disease (HFMD) is a contagious mainly occurring in young children, outbreaks commonly occur among children the Asia–Pacific region including Thailand. Moreover, World Health Organization (WHO) monitors HFMD Western Pacific to detect other significant events by Regional Event Based Surveillance System. caused group of enteroviruses (EVs) transmitted through direct contact (person person) indirect with contaminated objects (surface-to-hand). However, few studies have...

ObjectivesThis study aimed to evaluate a microfluidic paper-based analytical device (DEN-NS1-PAD) based on rapid NS1 antigen test for diagnosing dengue at the point of care.Methods219 serum samples from suspected cases were tested with developed DEN-NS1-PAD and commercial RDT by SD BIOLINE. The results compared nested-PCR results.ResultsThe limit detection was 0.78 ng mL−1. It showed 88.89% sensitivity, 86.67% specificity, substantial agreement correlation (κ = 0.7522) nested-PCR. In...