A5102019605- Hepatitis C virus research
- HIV/AIDS drug development and treatment
- Traditional and Medicinal Uses of Annonaceae
- Hepatitis B Virus Studies
- Monoclonal and Polyclonal Antibodies Research
- Marine Sponges and Natural Products
- Alcohol Consumption and Health Effects
- Cholinesterase and Neurodegenerative Diseases
- Head and Neck Cancer Studies
- Clostridium difficile and Clostridium perfringens research
- Viral-associated cancers and disorders
- Viral gastroenteritis research and epidemiology
- Salivary Gland Tumors Diagnosis and Treatment
- HIV/AIDS Research and Interventions
- Advanced Polymer Synthesis and Characterization
- HIV Research and Treatment
- Microscopic Colitis
- Bariatric Surgery and Outcomes
- Cancer therapeutics and mechanisms
- Fatty Acid Research and Health
- Listeria monocytogenes in Food Safety
- Flame retardant materials and properties
- Quinazolinone synthesis and applications
- Chromium effects and bioremediation
- Inflammatory Myopathies and Dermatomyositis
Min Sheng General Hospital
2009-2024
National Health Research Institutes
2018-2024
Institute of Biomedical Sciences, Academia Sinica
2022
Hungkuang University
2008-2020
National Tsing Hua University
2018
Tungs' Taichung MetroHarbor Hospital
2011-2016
Chung Chou University of Science and Technology
2016
Changhua Christian Hospital
2010-2013
National Pingtung University of Science and Technology
2013
Outcomes Research Consortium
2010
ABSTRACT The valine at position 82 (Val 82) in the active site of human immunodeficiency virus (HIV) protease mutates response to therapy with inhibitor ritonavir. By using X-ray crystal structure complex HIV and ritonavir, potent ABT-378, which has a diminished interaction Val 82, was designed. ABT-378 potently inhibited wild-type mutant ( K i = 1.3 3.6 pM), blocked replication laboratory clinical strains type 1 (50% effective concentration [EC 50 ], 0.006 0.017 μM), maintained high potency...
A-837093 is a potent and specific nonnucleoside inhibitor of the hepatitis C virus (HCV) nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase. It possesses nanomolar potencies in both enzymatic replicon-based cell culture assays. In rats dogs this compound demonstrated an oral plasma half-life greater than 7 h, its bioavailability was >60%. monkeys it had 1.9 h 15% bioavailability. Its antiviral efficacy evaluated two chimpanzees infected with HCV proof-of-concept study. The design...
Castration-resistant prostate cancer (CRPC) with sustained androgen receptor (AR) signaling remains a critical clinical challenge, despite depletion therapy. The Jumonji C-containing histone lysine demethylase family 4 (KDM4) members, KDM4A‒KDM4C, serve as coactivators of AR to promote tumor growth in and are candidate therapeutic targets overcome mutations/alterations-mediated resistance CRPC.In this study, using structure-based approach, we identified natural product, myricetin, able block...
Abstract Background The prevalence of resistance to fusidic acid methicillin-resistant Staphylococcus aureus (MRSA) was increased each year in a Taiwan hospital. Thirty-four MRSA clinical isolates collected 2007 and 2008 with reduced susceptibility FA were selected for further evaluation the presence determinants. Results most common determinant fusC , found 25 34 isolates. One acid-resistant harboured both fusB which is first time this has been identified. Mutations fusA 10 strains, total 3...
The first total synthesis of isopalhinine A, as well unified syntheses palhinine A and D, were successfully accomplished by means a biomimetic strategy that proceeds through bioinspired 5/6/6/9 tetracyclic intermediate, which mimics the amino ketone form D. An early-stage direct SN 2 cyclization to construct nine-membered azonane ring minimized transannular strain would otherwise be increased twisted nature isotwistane skeleton. Then, diastereoselective Diels-Alder reaction masked...
We have cloned Staphylococcus aureus DNA gyrase and topoisomerase IV expressed them in Escherichia coli as polyhistidine-tagged proteins to facilitate purification eliminate contamination by host enzymes. The enzyme preparations had specific activities similar previously reported values. Potassium glutamate (K-Glu) stimulated the drug-induced cleavage activity was optimal between 100 200 mM for peaked at IV. Higher concentrations of K-Glu inhibited both Using a common buffer system...
The radii of gyration wormlike "living polymer" micelles tetradecyltrimethylammonium bromide/salicylate have been measured in an elongational field at various elongation rates. radius the flow direction first increases, indicating alignment with field, reaching a maximum critical rate before falling. This decrease is taken as evidence for micelle scission high velocity gradients. microscopic structural measurements are consistent inferences drawn from rheological on similar systems.
(R)-9-[4-Hydroxy-2-(hydroxymethy)butyl]guanine (H2G) is a potent and selective inhibitor of herpesvirus replication. It nucleoside analog, its triphosphate derivative (H2G-TP) competitive DNA polymerases. In this study, the antiviral activities H2G acyclovir (ACV) development viral resistance to these agents were compared in varicella-zoster virus (VZV)-infected cells. plaque reduction assays, 50% effective concentration for VZV was 60- 400-fold lower than that ACV, depending on strain cell...
A series of bifunctional compounds have been discovered for their dual functionality as MER/AXL inhibitors and immune modulators. The furanopyrimidine scaffold, renowned its suitability in kinase inhibitor discovery, offers at least three distinct pharmacophore access points. Insights from molecular modeling studies guided hit-to-lead optimization, which revealed that the 1,3-diketone side chain hybridized with scaffold respectively combined amino-type substituent 1
We evaluated the heterogeneity of primary tumor volume (PTV) within tumors same pT4a-staged tongue carcinoma and to elucidate effects PTV on treatment outcomes in patients with carcinoma.Fifty-eight newly diagnosed who received surgery were enrolled onto this study. Magnetic resonance imaging-derived was measured by summation-of-area technique.The mean 24.55 ml, a range 5.32 119.64 ml. The receiver operating characteristic curve applied, optimal cutoff 23 Large associated significantly poor...
Objectives: Tophi may occur within the knee joint causing limited knee-joint range of motion (knee motion). We investigated relationships between motion, total intra-articular tophi size (tIA-tophi), and subcutaneous (tSC-tophi) determined improvement after continual urate-lowering therapy (ULT). Methods: A 26 patients with tophaceous gout were enrolled. Inclusion criteria age ≤ 60 years preserved space on a plain radiograph. tSC-tophi measured using Vernier caliper tIA-tophi magnetic...