A5030903871- Electron Spin Resonance Studies
- Photosynthetic Processes and Mechanisms
- Porphyrin and Phthalocyanine Chemistry
- Spectroscopy and Quantum Chemical Studies
- Photochemistry and Electron Transfer Studies
- Lanthanide and Transition Metal Complexes
- Hemoglobin structure and function
- Gas Sensing Nanomaterials and Sensors
- Electrochemical sensors and biosensors
- Magnetism in coordination complexes
- Analytical Chemistry and Sensors
University of Padua
2016-2020
We present a novel pulsed electron paramagnetic resonance (EPR) spectroscopic ruler to test the performance of recently developed spin-labeling method based on photoexcited triplet state (S=1). Four-pulse double (PELDOR) experiments are carried out series helical peptides, labeled at N-terminal end with porphyrin moiety, which can be excited state, and nitroxide various sequence positions, spanning distances in range 1.8-8 nm. The PELDOR traces provide accurate distance measurements for all...
Pulsed dipolar spectroscopic methods allow nanometer distance measurements between pairs of spin labels. We have proposed a new labelling approach, based on the population chromophore triplet under light excitation, testing it peptide-based ruler and photosynthetic protein. applied modified Electron DOuble Resonance (PELDOR) sequence where photogeneration precedes conventional 4-pulse PELDOR sequence. In this experiment, triplet-state serves as detection while stable nitroxide is pump spin....
Light-induced pulsed EPR dipolar spectroscopic methods allow the determination of nanometer distances between paramagnetic sites. Here we employ orthogonal spin labels, a chromophore triplet state and stable radical, to carry out distance measurements in singly nitroxide-labeled human neuroglobin. We demonstrate that Zn-substitution neuroglobin, populate Zn(II) protoporphyrin IX state, makes it possible perform light-induced experiments on hemeproteins, extending use spectroscopy this large...
The nature of the photoexcited triplet state free-base 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin (H2TPPS4-) has been investigated by advanced Electron Paramagnetic Resonance (EPR) techniques combined with quantum chemical calculations. zero-field splitting (ZFS) parameters, D and E, orientation transition dipole moment in ZFS tensor frame, proton hyperfine couplings have determined magnetophotoselection-EPR pulse electron-nuclear double resonance spectroscopy. Both time-resolved...
We report the observation of electron spin polarization transfer from triplet state a porphyrin to weakly coupled nitroxide radical in mutant human neuroglobin (NGB).
An investigation of the photoexcited triplet state chlorophyll (Chl) b has been carried out by means electron nuclear double resonance, both in a frozen organic solvent and protein environment provided water-soluble Lepidium virginicum. Density functional theory calculations have allowed complete assignment observed hyperfine couplings corresponding to methine protons methyl groups, leading picture spin density distribution tetrapyrrole macrocycle. The triplet-state properties Chl are found...
Invited for the cover of this issue is group Department Chemical Sciences at University Padova. The image depicts porphyrin-based spectroscopic ruler developed high-sensitive and accurate distance measurements (1.8–8 nm) by pulsed electron-electron double resonance spectroscopy (PELDOR/DEER) under light excitation. Read full text article 10.1002/chem.201603666.