A5030866663- Plant Virus Research Studies
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Gold and Silver Nanoparticles Synthesis and Applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Pesticide and Herbicide Environmental Studies
- Advanced biosensing and bioanalysis techniques
- Plant and Fungal Interactions Research
- Analytical chemistry methods development
- Biosensors and Analytical Detection
- Plant tissue culture and regeneration
Czech Academy of Sciences, Institute of Experimental Botany
2020-2024
Czech University of Life Sciences Prague
2020-2022
Biotemplated syntheses have emerged as an efficient strategy to control the assembly of metal nanoparticles (NPs) and generate promising plasmonic properties for sensing or biomedical applications. However, understanding nucleation growth mechanisms metallic nanostructures on biotemplate is essential prerequisite developing well-controlled nanotechnologies. Here, we used liquid cell Transmission Electron Microscopy (TEM) reveal how formation kinetics gold NPs affects their size density...
Methods for simple and fast assembly of exchangeable standard DNA parts using Type II S restriction enzymes are becoming more popular in plant synthetic molecular biology. These methods enable routine construction large complex multigene structures. Two available frameworks emphasize either high cloning capacity (Modular Cloning, MoClo) or simplicity (GoldenBraid, GB). Here we present a set novel α-level plasmids compatible with the GB convention that extend ability to rapidly assemble...
Reverse transcription PCR (RT-PCR) is a popular method for detecting RNA viruses in plants. RT-PCR usually performed classical two-step procedure: the first step, cDNA synthesized by reverse transcriptase (RT), followed amplification thermostable polymerase separate tube second step. However, one-step kits containing multiple enzymes optimized RT and single can also be used. Here, we describe an single-enzyme assay based on RTX DNA that has both activities. The expression plasmid...
The employment of biomolecular templates for the synthesizing nanohybrid constructs is expanding, driven by their prospective uses in biosensing and biomedical fields. Gold nanoparticles (AuNPs) and, particular, assemblies are especially preferred Surface Enhanced Raman Spectroscopy (SERS) because ability to amplify signals through localized surface plasmon resonances, thus enabling detection molecules at exceedingly low concentrations. Our investigative approach dedicated studing role...
We have developed a Potato virus X (PVX)-based vector system compatible with the GoldenBraid 2.0 (GB) cloning strategy to transiently express heterologous proteins or peptides in plants for biotechnological purposes. This consists of three domestication vectors carrying GB parts-the cauliflower mosaic (CaMV) 35S promoter PVX upstream second subgenomic coat protein (PVX CP SGP), nopaline synthase (NOS) terminator downstream first SGP and gene interest (GOI). The full-length clone sequence...