A5055293662- Genomics and Rare Diseases
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- RNA regulation and disease
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Virus-based gene therapy research
- Genetics, Bioinformatics, and Biomedical Research
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Biomedical and Engineering Education
- Gene Regulatory Network Analysis
- Educational Leadership and Innovation
- Transgenic Plants and Applications
- Agricultural and Food Sciences
- Genetic Syndromes and Imprinting
- vaccines and immunoinformatics approaches
- Banana Cultivation and Research
- Agriculture, Land Use, Rural Development
- Cancer-related molecular mechanisms research
- Receptor Mechanisms and Signaling
- Science, Research, and Medicine
- ATP Synthase and ATPases Research
Johns Hopkins Medicine
2016-2022
Johns Hopkins University
2016-2022
Broad Center
2020-2022
University of California, San Francisco
2020-2022
Neurological Surgery
2022
Universidad Privada de Santa Cruz de la Sierra
2019-2020
University of Baltimore
2020
Children's National
2011-2012
University of California, Santa Barbara
2012
Kabuki syndrome is a Mendelian intellectual disability caused by mutations in either of two genes (KMT2D and KDM6A) involved chromatin accessibility. We previously showed that an agent promotes opening, the histone deacetylase inhibitor (HDACi) AR-42, ameliorates deficiency adult neurogenesis granule cell layer dentate gyrus rescues hippocampal memory defects mouse model (Kmt2d+/βGeo). Unlike drug, dietary intervention could be quickly transitioned to clinic. Therefore, we have explored...
Abstract Background The cell cycle is a highly conserved, continuous process which controls faithful replication and division of cells. Single-cell technologies have enabled increasingly precise measurements the both as biological interest possible confounding factor. Despite its importance conservation, there no universally applicable approach to infer position in with high-resolution from single-cell RNA-seq data. Results Here, we present tricycle, an R/Bioconductor package, address this...
Chromatin modifiers act to coordinate gene expression changes critical neuronal differentiation from neural stem/progenitor cells (NSPCs). Lysine-specific methyltransferase 2D (KMT2D) encodes a histone that promotes transcriptional activation and is frequently mutated in cancers the majority (>70%) of patients diagnosed with congenital, multisystem intellectual disability disorder Kabuki syndrome 1 (KS1). Critical roles for KMT2D are established various non-neural tissues, but effects loss...
Human chromosomes are pervasively transcribed, but systematic understanding of coding and lncRNA genome function in cell differentiation is lacking. Using CRISPR interference (CRISPRi) human induced pluripotent stem cells, we performed dual genome-wide screens - assessing 18,905 protein-coding 10,678 loci identified 419 201 genes that regulate neural induction. Integrative analyses revealed distinct properties function, including a 10-fold enrichment for roles compared to proliferation....
ABSTRACT Previous studies have shown that brief access to cocaine yields an increase in D2 receptor binding the medial prefrontal cortex (mPFC), but extended results normalized of receptors (i.e. returned control levels). Extended‐access conditions also been produce increased expression NR2 subunit N‐Methyl‐D‐aspartate mPFC. These implicate disrupted glutamate and dopamine function within this area. Therefore, present study, we monitored content mPFC during, or 24 hours after,...
Abstract Particular challenges exist for science education in the developing world, where limited resources require curricula designed to balance state-of-the-art knowledge with practical and political considerations region-specific contexts. Project-based biology teaching is especially difficult execute due high infrastructural costs teacher training. Here, we report results of implementing short, challenging, low-cost courses school college students Bolivia, taught collaboration between...
The 4H syndrome (hypomyelination, hypodontia, hypogonadotropic hypogonadism) is a newly recognized leukodystrophy. classical form characterized by the association of hypomyelination, abnormal dentition, and hypogonadism, but recent identification 2 genes responsible for demonstrates that these 3 main characteristics can be variably combined among "Pol-III (polymerase III)-related leukodystrophies." pathophysiology this group diseases still to elucidated, there are no neuropathologic...
Abstract The atmospheric pressure that decreases with altitude affects lung physiology. However, these changes in physiology are not usually considered ventilator design and testing. We argue high human populations require special attention to access the international supply of ventilators.
OPINION article Front. Educ., 03 September 2019Sec. STEM Education Volume 4 - 2019 | https://doi.org/10.3389/feduc.2019.00095
Abstract Transcriptional and epigenetic regulators shape the chromatin microenvironment corresponding gene expression during cellular differentiation homeostasis. Programmable modulators of transcription provide a powerful toolkit for controlling dosage in therapeutic applications, but limited catalog functional domains constrains their robustness durability profiles, large cargo sizes impede clinical delivery. To address these limitations, here we perform high-throughput screening to...
ABSTRACT Background The cell cycle is a highly conserved, continuous process which controls faithful replication and division of cells. Single-cell technologies have enabled increasingly precise measurements the both as biological interest possible confounding factor. Despite its importance conservation, there no universally applicable approach to infer position in with high-resolution from single-cell RNA-seq data. Results Here, we present tricycle, an R/Bioconductor package, address this...
Abstract Chromatin modifiers act to coordinate gene expression changes critical neuronal differentiation from neural stem/progenitor cells (NSPCs). Lysine-specific methyltransferase 2D (KMT2D) encodes a histone that promotes transcriptional activation, and is frequently mutated in cancers the majority (>70%) of patients diagnosed with congenital, multisystem intellectual disability (ID) disorder Kabuki syndrome 1 (KS1). Critical roles for KMT2D are established various non-neural tissues,...
Abstract Programmable epigenetic modulators provide a powerful toolkit for controlling gene expression in novel therapeutic applications, but recent discovery efforts have primarily selected potency of effect rather than contextual robustness or durability thereof. Current CRISPR-based tools are further limited by large cargo sizes that impede clinical delivery and, activation contexts, brief activity windows preclude transient, single-dose strategies such as lipid nanoparticle (LNP)...
ABSTRACT Particular challenges exist for science education in the developing world, where limited resources beget curricula designed to balance state-of-the-art knowledge with practical and political considerations region-specific contexts. Project-based biology teaching is particularly difficult execute due high infrastructural costs teacher training. Here, we report our results implementing short, challenging, low-cost courses school college students Bolivia, taught collaboration between...
Sustainable food production in developing countries is challenging, as it requires balancing scalability with respect for local culture and traditions. Bolivia represents a particularly interesting example; over 36 recognized indigenous groups encompassing 70% of the country’s population relatively small territory, considered one most diverse world (Ferreira et al., 2019). Agriculture employs 5% 14% its GDP. Yet, introduction new agribusiness approaches has often been met resistance by...
Abstract Immunology is typically first encountered by students in advanced college or graduate courses. Additional challenges to teaching immunology exist the developing world, where limited resources require curricula balancing state-of-the-art knowledge with region-specific practical considerations. Project-based especially difficult execute due lack of infrastructure and teacher training. Here, we report results implementing short hands-on low-cost microbiology courses for high school...