The Hawaiian red algal flora is diverse, isolated, and well studied from a morphological anatomical perspective, making it an excellent candidate for assessment using combination of traditional taxonomic molecular approaches. Acquiring these biodiversity data freely available in timely manner ensures that other researchers can incorporate baseline findings into phylogeographic studies algae or found locations. A total 1,946 accessions are represented the collections 305 different...

A second species in the siphonous green algal genus Avrainvillea was recently discovered off island of O‘ahu Main Hawaiian Islands. Specimens were collected from Honolulu Harbor, including its entrance channel, and near Ke‘ehi Harbor. These locations are both Mālama Bay on O‘ahu’s south shore or adjacent to urbanized estuaries, respectively. In situ observations, morphological molecular assessments conducted examine alga’s habit distribution, as well assess putative identification. The alga...

In spite of their ecological importance as primary producers and microbioeroders marine calcium carbonate (CaCO3) substrata, endolithic phototrophs spanning both prokaryotic (the cyanobacteria) eukaryotic algae lack established molecular resources for facilitated survey with high throughput sequencing. Here, the development a metabarcoding framework elongation factor EF-Ttu (tufA) was tested on four Illumina-sequenced CaCO3 microfloras characterization phototrophs, especially abundant...

Rhodoliths are the main hard substrata for attachment of benthic macroalgae in NW Gulf Mexico rubble habitats that associated with salt domes, unique deep bank at ~50-90m depth on continental shelf offshore Louisiana and Texas. With advent additional sequencing technologies, methodologies biodiversity assessments now rapidly shifting to DNA metabarcoding, i.e. High Throughput Sequencing (HTS) environmental mixtures standardized molecular markers, such as 16S V4, rapid, cost-effective...

The red alga Gracilaria salicornia (C. Agardh) E. Y. Dawson was introduced intentionally to two reefs on O'ahu, Hawai'i, in the 1970s for experimental aquaculture agar industry. Some 30 yr later, this species has spread from initial sites of introduction and is now competing with native marine flora fauna. goals study were quantify various aspects G. ecology Hawai'i an effort develop control or eradication tools. Experimental plots established determine cover biomass per square meter amount...

The diversity of seaweeds and decapod crustaceans associated with rhodoliths on deep offshore banks in the northwestern Gulf Mexico decreased dramatically after Macondo blowout. Decapod crustacean communities declined both abundance exhibited major shifts species dominance. Rhodoliths appear to serve as seedbanks for biological because dead rhodolith rubble became covered by epi- endolithic algae microbes laboratory microcosms. Decreased seaweed field may relate nutrient availability...

In the northwestern Gulf of Mexico, beds rhodoliths and unconsolidated rubble at 55–70 m depth are associated with unique offshore deep bank habitats known as salt domes or diapirs. Prior to 2010 BP Deepwater Horizon oil spill these harbored highest seaweed diversity in northern Mexico. Six post-spill cruises led Louisiana two sites previously documented rich algal assemblages (i.e. prespill) revealed a dramatic die-off seaweeds both sites, dredged appearing bleached mostly denuded fleshy...

Interspecific systematics in the red algal order Sporolithales remains problematic. To re-evaluate its species, DNA analyses were performed on historical type material and recently collected specimens assigned to two genera Sporolithon Heydrichia. Partial rbcL sequences from lectotype of ptychoides (the generitype species) molle, both El Tor, Egypt, are exact matches field-collected topotype specimens. crassum erythraeum also have same locality; former appears no longer exist, we unable PCR...

The genus Pseudochlorodesmis (Bryopsidales) is composed of diminutive siphons extreme morphological simplicity. discovery Pseudochlorodesmis-like juveniles in more complex Bryopsidales (e.g., the Halimeda microthallus stage) jeopardized recognition this genus. Confronted with uncertainty, taxonomists transferred many simple into a new genus, Siphonogramen. In study, we used multimarker approach to clarify phylogenetic and taxonomic affinities Pseudochlorodesmis-Siphonogramen (PS) within...

Sauvage T., Payri C., Draisma S.G.A., Prud′homme van Reine W.F., Verbruggen H., Belton G.S., Gurgel C.F.D., Gabriel D., Sherwood A.R. and Fredericq S. 2013. Molecular diversity of the Caulerpa racemosa–Caulerpa peltata complex (Caulerpaceae, Bryopsidales) in New Caledonia, with new Australasian records for C. racemosa var. cylindracea. Phycologia 52: 6–13. DOI: 10.2216/11-116.1Molecular characterization (plastid-encoded tufA gene) Caledonian members identified five lineages, each possibly...

Three related new alkylphenols, termed anaephenes A–C (1–3), containing different side chains, were isolated from an undescribed filamentous cyanobacterium (VPG 16-59) collected in Guam. Our 16S rDNA sequencing efforts indicated that VPG 16-59 is a member of the marine genus Hormoscilla (Oscillatoriales). The structures (1–3) elucidated by spectroscopic methods, and compounds assayed for growth inhibitory activity against prokaryotic eukaryotic cell lines. Anaephene B (2), possessing...

Oxford nanopore Technologies (ONT) provides three main library preparation strategies to sequence bacterial genomes. These include tagmentation (TAG), ligation (LIG) and amplification (PCR). Despite ONT's recommendations, making an informed decision for choice remains difficult without a side-by-side comparison. Here, we sequenced 12 strains examine the overall output of these strategies, including sequencing noise, barcoding efficiency assembly quality based on mapping curated genomes...

The siphonous green algal family C aulerpaceae includes the monotypic genus aulerpella and species‐rich aulerpa . A molecular phylogeny was inferred from chloroplast tuf rbc L DNA sequences analyzed together with a five marker dataset of non‐caulerpacean algae. Six lineages were revealed, but relationships between them remained largely unresolved. clade representing multiple cryptic species nested within Therefore, that is subsumed ambigua Okamura reinstated. subgenus status proposed for six...

Dysidazirine carboxylic acid (1) was isolated from the lipophilic extract of a collection benthic marine cyanobacterium Caldora sp. reefs near Fort Lauderdale, Florida. The planar structure this new compound determined by spectroscopic methods and comparisons between HRMS NMR data with its reported methyl ester. absolute configuration single chiral center conversion 1 to ester comparison specific rotation two known isomers, 2 3. Molecular sequencing 16S rDNA indicated that differs...

In the Northwestern Gulf of Mexico (NWGMx), subtidal rhodolith beds offshore Louisiana at 45–80 m depth harbor a diverse community uncharacterized non-geniculate coralline algae including both biogenic and autogenic rhodoliths other encrusting taxa. Identifying specimens to their correct genus species is an ongoing process because many available names remain be validated by comparison type specimens. Here, comparative DNA sequencing (psbA, UPA, COI) scanning electron microscopy (SEM) are...

Abstract Rhodoliths are benthic calcium carbonate nodules accreted by crustose coralline red algae which recently have been identified as useful indicators of biomineral changes resulting from global climate change and ocean acidification. This study highlights the discovery that interior rhodoliths marine biodiversity hotspots function seedbanks temporary reservoirs previously unknown stages in life history ecologically important dinoflagellate haptophyte microalgae. Whereas studied...

An extensive study of the secondary metabolites produced by a new Sticta sp. lichen has led to isolation three compounds containing 4-amino-3-hydroxy-5-phenylpentanoic acid residue (Ahppa). The structures stictamides A-C (1-3) were assigned 2D NMR spectroscopic and chemical methods. Due epimerization Ahppa observed after hydrolysis, configuration this unit was deduced through conversion 1 an appropriate derivative application our recently developed statine database. Evaluation stictamide A...

Abstract In 2011, the Indian River Lagoon, a biodiverse estuary in eastern Florida (USA), experienced an intense microalgal bloom with disastrous ecological consequences. The included mix of microalgae unresolved taxonomy and lasted for 7 months maximum concentration 130 μg chlorophyll L −1 . 2012, brown tide Aureoumbra lagunensis also bloomed portions this estuary, reoccurrences 2016 2018. To identify understand role grazer pressure (top-down control) on formation, we coupled DNA sequencing...

Our ongoing efforts to explore the chemical space associated with marine cyanobacteria from coral reefs of Guam have yielded two new members anaenamide family natural products, anaenamides C (3) and D (4). These compounds were isolated a novel Hormoscilla sp. (VPG16-58). phylogenetic profiling (16S rDNA) this cyanobacterium indicated that VPG16-58 is taxonomically distinct previously reported producer anaephenes, VPG16-59 (Hormoscilla sp.), other documented species genus Hormoscilla. The...

The MinION Access Program (MAP, 2014-2016) allowed selected users to test the prospects of long nanopore reads for diverse organisms and applications through rapid development improving chemistries. In 2014, faced with a fragmented Illumina assembly chloroplast genome green algal holobiont Caulerpa ashmeadii, we applied MAP investigate such intricacies, as well further explore hologenome this species native hybrid approaches.The could only be resolved circular molecule in assemblies, which...

Gibsmithia hawaiiensis is a peculiar red alga characterized by furry gelatinous lobes arising from cartilaginous stalk. The species has been recorded tropical reef systems throughout the Indo-Pacific. A multilocus phylogeny (UPA, rbcL, COI-5P) of 36 specimens collected distribution range, showed high genetic diversity at level. Two major groups were identified, each consisting multiple lineages. Genetic variability was low in Hawaiian Islands and northern Red Sea Western Indian Ocean Coral...

The tropical alga previously recognized as Gibsmithia hawaiiensis (Dumontiaceae, Rhodophyta) was recently suggested to represent a complex of species distributed throughout the Indo-Pacific Ocean and characterized by peculiar combination hairy (pilose) gelatinous lobes growing on cartilaginous stalks. Phylogenetic reconstructions based three genetic markers are presented here with inclusion new samples. Further diversity is reported within complex, nine lineages spread in four major...

In the past, non-geniculate coralline algae in northwestern Gulf of Mexico have been identified based primarily on comparative morpho-anatomy. Recent studies employing DNA sequencing techniques combined with morpho-anatomical using SEM revealed a wealth previously undocumented diversity rhodolith-forming Corallinales, Hapalidiales and Sporolithales from mesophotic hard bank communities at 45-90 meters depth. Although many advances last decade made clarifying species names describing new...