A5008445546- Electrospun Nanofibers in Biomedical Applications
- Cardiac Valve Diseases and Treatments
- Tissue Engineering and Regenerative Medicine
- Connective tissue disorders research
- Cardiovascular Function and Risk Factors
- Aortic Disease and Treatment Approaches
- Infective Endocarditis Diagnosis and Management
- Protease and Inhibitor Mechanisms
- Infectious Aortic and Vascular Conditions
- Angiogenesis and VEGF in Cancer
- Aortic aneurysm repair treatments
- Coronary Interventions and Diagnostics
- Dermatological and Skeletal Disorders
- Cardiac Fibrosis and Remodeling
- Elasticity and Material Modeling
- Dental materials and restorations
- Cardiac Structural Anomalies and Repair
- Influenza Virus Research Studies
- Cardiac and Coronary Surgery Techniques
- Congenital Heart Disease Studies
- Bone and Dental Protein Studies
- Mesenchymal stem cell research
- Congenital heart defects research
- Apelin-related biomedical research
- Renal and related cancers
Clemson University
2014-2023
Universitatea de Medicină, Farmacie, Științe și Tehnologie „George Emil Palade” din Târgu Mureș
1993-2021
Biocom
2012-2021
Romanian Academy
2020
Golden Jubilee National Hospital
2016
Instituto de Neurologia de Curitiba
2016
Prisma Health
2012
Clinical Emergency Hospital Bucharest
2006-2007
Academy of Medical Sciences
1991
Scaffolds for heart valve tissue engineering must function immediately after implantation but also need to tolerate cell infiltration and gradual remodeling. We hypothesized that moderately cross-linked collagen scaffolds would fulfill these requirements. To test our hypothesis, prepared from decellularized porcine pericardium were treated with penta-galloyl glucose (PGG), a collagen-binding polyphenol, tested biodegradation, biaxial mechanical properties, in vivo biocompatibility. For...
Abstract Small carbon nano‐onions (CNOs, 6–8 shells) were prepared in high yield and functionalized with carboxylic groups by chemical oxidation. After functionalization these nanostructures soluble aqueous solutions. 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2 tetrazolium (MTS) tests showed excellent cytocompatibility of all CNOs analyzed at 30 300 μg mL −1 , so can be safely used for biological applications. The first covalent oxidized (ox‐CNOs)...
Tissue engineering holds great promise for treatment of valvular diseases. Despite excellent progress in the field, current approaches do not fully take into account each patient's valve anatomical uniqueness, presence a middle spongiosa cushion that allows shearing external fibrous layers (fibrosa and ventricularis), need autologous interstitial cells. In this study we propose novel approach to heart tissue based on bioreactor conditioning mesenchymal stem cell-seeded, valve-shaped...
Heart valve tissue engineering requires biocompatible and hemocompatible scaffolds that undergo remodeling repopulation, but also withstand harsh mechanical forces immediately following implantation. We hypothesized reversibly stabilized acellular porcine valves, seeded with endothelial cells conditioned in pulsatile bioreactors would pave the way for next generations of engineered heart valves (TEHVs). A novel conditioning system was first designed, manufactured tested to adequately assess...
There is a great need for living valve replacements patients of all ages. Such constructs could be built by tissue engineering, with perspective the unique structure and biology aortic root. The root composed several different tissues, careful structural functional consideration has to given each segment component. Previous work shown that immersion techniques are inadequate whole-root decellularization, wall being particularly resistant decellularization. aim this study was develop...
Tissue-engineered vascular grafts require elastic, acellular porous scaffolds with controlled biodegradability and properties matching those of natural arteries. Elastin would be a desirable component for such applications, but elastin does not easily regenerate experimentally. Our approach is to develop tubular using decellularization removal collagen from porcine carotid arteries (∼5 mm diameter) alkaline extraction. Because susceptible rapid degeneration after implantation, were further...
Tissue engineering employs scaffolds, cells, and stimuli brought together in such a way as to mimic the functional architecture of target tissue or organ. Exhilarating advances regenerative medicine allow us envision vitro creation vivo regeneration cardiovascular tissues. Such accomplishments have potential revolutionize greatly improve our standard life. However, enthusiasm has been hampered recent years because abnormal reactions at implant-host interface, including cell proliferation,...
There is a significant clinical need for new approaches to treatment of mitral valve disease. The aim this study was develop tissue-engineered scaffold possessing appropriate composition and structure ensure ideal characteristics valves, such as large orifice, rapid opening closure, maintenance annulus–papillary muscle continuity, in vivo biocompatibility extended durability. An extracellular matrix-based generated, based on the native porcine starting material technique cell removal without...
Abstract Glutaraldehyde‐crosslinked bovine pericardium is widely used in bioprosthetic heart valve fabrication. In an attempt to set a scientific basis for more reproducible tissue selection, we produced and analyzed topographical maps of glutaraldehyde‐treated pericardium. Whole pericardia were divided into specific anatomical areas their thickness was measured mapped on templates. each area, the suture holding power determined both parallel perpendicular (to base‐apex line) directions;...
Abstract Crosslinking of collagenous biomaterials currently employs the use glutaraldehyde. The putative enhancement glutaraldehyde crosslinking by lysine was investigated in three model systems: bovine pericardium, collagen membranes, and serum albumin. Repetitive sequential treatment pericardium with finally formaldehyde produced a matrix which, two criteria used (shrinkage temperature urea/SDS soluble collagen), shown to be more highly crosslinked than fixed alone. Essentially same...
Degeneration processes that affect bioprosthetic heart valves made from glutaraldehyde treated bovine pericardium are poorly understood. The present study undertook the identification and characterization of matrix metalloproteinases (MMPs) in extracts obtained 28 pericardial derived explanted at surgery. A lysosomal marker was used to assess incidence infiltrating extracellular degrading cells. major biochemical features were associated with tissue degeneration valve failure increased...
There is a great need for acellular, fully vascularized, and biocompatible myocardial scaffolds that provide agreeable biological, nutritional, biomechanical niches reseeded cells in vitro vivo applications. We generated flap comprising porcine left-anterior ventricular myocardium its associated coronary arteries veins investigated the combinatorial effects of sodium dodecyl sulfate (SDS) hydroxide (NaOH) perfusion on both extracellular matrix (ECM) vascular ECM. Results showed all displayed...
Diabetes contributes directly to the development of cardiovascular aortic valve disease. There is currently no drug therapy available for a dysfunctional and this urges need additional research identify distinctive mechanisms disease evolution. The aim study was evaluate changes valvular lesions induced in hyperlipemic ApoE −/− mouse model by early type 1 diabetes onset (at 4 7 days after streptozotocin induction). haemodynamic parameters were evaluated echography blood samples valves...