Struct2Net is a web server for predicting interactions between arbitrary protein pairs using structure-based approach. Prediction of protein–protein (PPIs) central area interest and successful prediction would provide leads experiments drug design; however, the experimental coverage PPI interactome remains inadequate. We believe that first community-wide resource to predictions go beyond homology modeling. Also, most web-resources PPIs currently rely on functional genomic data (e.g. GO...

The t(14;18) translocation in follicular lymphoma is one of the most common chromosomal translocations. Most breaks on chromosome 18 are located at 3′-UTR BCL2 gene and mainly clustered major breakpoint region (MBR). Recently, we found that MBR has a non-B DNA character genomic DNA. Here, show single-stranded modeled from template strand MBR, forms secondary structures migrate faster native PAGE presence potassium, due to formation intramolecular G-quadruplexes. Circular dichroism shows...

Abstract We have studied here using a number of biophysical tools the effects osmolytes, betaine, citrulline, proline and sorbitol which differ significantly in terms their physical characteristics such as, charge distribution, polarity, H-bonding abilities etc, on fibrillation insulin. Among these, citrulline are very effective decreasing extent fibrillation. Proline also causes substantial delay onset concentration range (50–250 mM) whereas an effect is seen for only at 250 mM case betaine...

Abstract Two‐dimensional NMR has developed into an important tool for the study of solution structures nucleic acids. The conformational information is generally derived from three bond coupling constants (J) and interproton distances (r). Strategies have been to obtain J values using COSY J‐resolved spectroscopy. Estimation involves measurement NOESY build‐up curves as a function mixing time. This can be coupled with structural refinement techniques three‐dimensional structure Here we...

Sequence specific resonance assignment is the primary requirement for all investigations of proteins by NMR methods. In present postgenomic era where structural genomics and protein folding have occupied center stage research, there a high demand on speed assignment, whereas presently available methods based either NOESY or some triple-resonance experiments are rather slow. They also limited success with unfolded because lack NOEs, poor dispersion amide carbon chemical shifts. This paper...

A new approach based on the correlated spectroscopy (COSY) in 2D NMR has been described for determination of sugar geometries oligonucleotides. Under usual low resolution conditions employed COSY, intensities cross peaks depend magnitudes coupling constants. There are five vicinal constants a deoxyribose ring which sensitive to geometry. The presence, absence and rough comparison relative COSY arising from such enable one fix conformation fair degree precision. methodology applied...

Hydrogen bonding interactions are one of the most important chemical among materials, especially biological which help confer specificity, is crucial for their efficient functioning. Recently, low-barrier hydrogen bonds (LBHBs) have been proposed to play a critical role in enzyme catalysis. In this review, tools identify LBHBs described, along with analyses neutron crystal structures small molecules geometric parameters characteristic LBHBs, assumed be characterized by dynamic disorder bond...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel solution conformation of DNA observed in d(GAATTCGAATTC) by two-dimensional NMR spectroscopyK. V. R. Chary, Hosur, Girjesh Govil, Zu Kun Tan, and H. T. MilesCite this: Biochemistry 1987, 26, 5, 1315–1322Publication Date (Print):March 1, 1987Publication History Published online1 May 2002Published inissue 1 March 1987https://pubs.acs.org/doi/10.1021/bi00379a017https://doi.org/10.1021/bi00379a017research-articleACS PublicationsRequest reuse...

Dynamin is a GTP-binding protein whose oligomerization-dependent assembly around the necks of lipid vesicles mediates their scission from parent membranes. thus directly involved in regulation endocytosis. Sumoylation post-translational modification whereby ubiquitin-like modifier Sumo covalently attached to lysine residues on target proteins by process requiring concerted action an activating enzyme (ubiquitin-activating enzyme), conjugating (ubiquitin carrier protein), and ligating...

Non-uniform sampling in combination with homonuclear broadband decoupling along an indirect dimension, and covariance processing are used to record ultrahigh resolution two-dimensional TOCSY spectra less than half hour, for typical sample concentrations the mm range. correlations belonging protons separated by as little ≈2 Hz can be distinctly discerned. The utility of technique low has been demonstrated.

We report here interesting synergistic effects of proline and sorbitol, two well-known chemical chaperones, in the inhibition fibrillation proteins, insulin lysozyme. A combination many biophysical techniques has been used to understand structural morphology modes interaction chaperones with proteins during fibrillation. Both establish stronger polar interactions elongation saturation stages compared that native stage. However, when presented as a mixture, we also see contribution...

Autoprocessing of HIV-1 protease (PR) precursors is a crucial step in the generation mature protease. Very little known regarding molecular mechanism and regulation this important process viral life cycle. In context we report here first complete residue level investigations on structural folding characteristics 17-kDa precursor TFR-PR-Cnn (161 residues) The shows autoprocessing activity indicating that solution has certain population folded active dimer. Removal 5-residue extension, Cnn at...

Complete resonance assignments of the proton NMR spectrum gonadoliberin (in its native amide and free acid forms) have been obtained using two-dimensional nuclear magnetic spectroscopy under three different environmental conditions, namely, dimethyl sulphoxide solution, aqueous solution lipid-bound form in model membranes. The chemical shifts cases compared to derive information about inherent conformational characteristics molecule. It has inferred that molecule possesses no short-range or...

The assignment of the 1 H nuclear magnetic resonance (NMR) spectrum cardiotoxin V II 2 from Naja mossambica is described and documented. assignments are based entirely on amino acid sequence two‐dimensional NMR experiments at 500 MHz. Individual were obtained 45 C for backbone protons 56 out total 60 residues, exceptions being N‐terminal dipeptide segment Leu‐1–Lys‐2–, Pro‐8 Pro‐15. Complete non‐labile hydrogen atoms side chains 37 Asn‐4 Asn‐19 δ amide also identified. For 19 long individual...