A5062192496- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Pelvic floor disorders treatments
- Urinary Tract Infections Management
- Urinary Bladder and Prostate Research
- Emergency and Acute Care Studies
- Trauma and Emergency Care Studies
- Silk-based biomaterials and applications
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Trauma Management and Diagnosis
The University of Texas MD Anderson Cancer Center
2011-2020
The University of Texas at Austin
2019-2020
Abstract High-throughput CRISPR-Cas9 knockout screens using a tiling-sgRNA design permit in situ evaluation of protein domain function. Here, to facilitate de novo identification essential domains from such screens, we propose ProTiler, computational method for the robust mapping CRISPR hyper-sensitive (CKHS) regions, which refer regions associated with strong sgRNA dropout effect screens. Applied published tiling screen dataset, ProTiler identifies 175 CKHS 83 proteins. Of these more than...
A multifaceted strategy using a composite of anti-cancer nanotherapeutic and natural biomaterials silk fibroin (SF) chitosan (CS) blend scaffolds was investigated for the treatment tissue defect post-tumor resection by providing local release therapeutic filling site with regenerative bioscaffolds. The scaffold-emodin nanoparticle composites were fabricated characterized drug entrapment release, mechanical strength, efficacy against GILM2 breast cancer cells in vitro vivo rat tumor model....