A5003687145- Flood Risk Assessment and Management
- Atmospheric and Environmental Gas Dynamics
- Marine and coastal ecosystems
- Connective tissue disorders research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Hydrology and Watershed Management Studies
- Climate change and permafrost
- Pleural and Pulmonary Diseases
- Neonatal Respiratory Health Research
- Remote Sensing in Agriculture
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Medical Imaging and Pathology Studies
- Isotope Analysis in Ecology
- Methane Hydrates and Related Phenomena
- Inhalation and Respiratory Drug Delivery
- Occupational and environmental lung diseases
- Arctic and Antarctic ice dynamics
- Water Quality Monitoring and Analysis
- Microbial Community Ecology and Physiology
- Peptidase Inhibition and Analysis
- Water Quality Monitoring Technologies
- Soil and Water Nutrient Dynamics
- Tropical and Extratropical Cyclones Research
- Remote Sensing and Land Use
University of Washington
2017-2022
Custom Electronics (United States)
2022
Seattle University
2020
ORCID
2020
St. Cloud Hospital
2018
Yale University
1997-2017
Heidelberg University
2007
Memorial Hospital of Rhode Island
1991-2001
Brown University
1993-2001
Miriam Hospital
1996
SignificanceStream/river carbon dioxide (CO2) emission has significant spatial and seasonal variations critical for understanding its macroecosystem controls plumbing of the terrestrial budget. We relied on direct fluvial CO2 partial pressure measurements seasonally varying gas transfer velocity river network surface area estimates to resolve reach-level flux at global scale. The percentage primary production (GPP) shunted into rivers that ultimately contributes evasion increases with...
Interleukin-11 is a pleotropic cytokine produced by lung stromal cells in response to respiratory viruses, cytokines, and histamine. To further define its potential effector functions, the Clara cell 10-kD protein promoter was used express IL-11 airways of resulting transgene mice were characterized. In contrast (-) littermates, (+) animals manifest nodular peribronchiolar mononuclear infiltrates impressive remodeling with subepithelial fibrosis. The inflammatory foci contained large numbers...
Abstract Satellite estimates of inland water quality have the potential to vastly expand our ability observe and monitor dynamics large bodies. For almost 50 years, we been able remotely sense key constituents like total suspended sediment, dissolved organic carbon, chlorophyll a, Secchi disk depth. Nonetheless, remote sensing is poorly integrated into sciences, in part due a lack publicly available training data perception that are unreliable. Remote models can be improved by validation on...
Standard overexpression transgenic approaches are limited in their ability to model waxing and waning diseases frequently superimpose development-dependent -independent phenotypic manifestations. We used the clara cell 10-kD protein (CC10) promoter reverse tetracycline transactivator (rtTA) create a lung-specific, externally regulatable, system this express human interleukin 11 (IL-11) respiratory structures. Gene induction could be achieved utero, neonates adult animals. Moreover, gene...
Excessive collagen deposition plays a critical role in the development of fibrosis, and early or active fibrosis may be more susceptible to therapeutic intervention than later stages scarring. However, at present there is no simple method for assessing collagen-synthesizing secreting activity fibroblasts human tissues. Type I procollagen carboxyterminal domains are proteolytically removed during secretion. Thus, antibodies these should stain synthesizing type but not extracellular fibrils...
Bleomycin was administered by intratracheal injection to hamsters. Thirty days after a single of 1 unit, the lungs showed patchy fibrosis that involved air spaces, terminal bronchioles, and pleura. Connective tissue stains indicated increased amounts collagen elastin in fibrotic areas. Biochemical analysis revealed both were more than 2-fold above control values. The number desmosine cross links per molecule same treated with bleomycin. concentrations 2 units or resulted high mortality...
Radioiodinated leukocyte elastase was found to bind rapidly and specifically alveolar macrophages in vitro. In contrast the binding of pancreatic bacterial proteases, did not require presence alpha 2 macroglobulin. The inhibited by an excess unlabeled enzyme saturable increasing concentrations. Leukocyte thus met criteria for receptor-mediated binding, with estimated association constant 4.97 x 10(5) M-1 total 640 10(6) sites/cell. It differed from previously described lysosomal glycosidases...
Human pulmonary alveolar macrophages synthesized and secreted several characteristic high molecular weight proteins for at least 7 d in vitro. Immunoprecipitates of medium cell lysates from metabolically labeled cultures with specific anti-human plasma fibronectin IgG contained one major polypeptide 440,000 (unreduced) or 220,000 (reduced). An identical conditioned radiolabeled bound specifically to gelatin-Sepharose, demonstrating that a molecule immunologically functionally similar...
The highest concentration of the world's lakes are found in Arctic-boreal regions [C. Verpoorter, T. Kutser, D. A. Seekell, L. J. Tranvik, Geophys. Res. Lett. 41, 6396-6402 (2014)], and consequently undergoing most rapid warming [J. E. Overland et al., Arctic Report Card (2018)]. However, ecological response to remains highly uncertain. Historical trends lake color from remote sensing observations can provide insights into changing ecology, yet have not been examined at pan-Arctic scale....
Mouse alveolar macrophages (AM) cultured in the absence of serum secrete an elastolytic enzyme. The elastase from AM resembles previously described peritoneal (PM) pH optimum and inhibition profile. macrophage enzymes do not appear to be stored, with periodic changes culture medium, accumulate extracellularly for up 10 days. Resident PM produce barely detectable levels extracellular unless given a phagocytic load. Thioglycollate-stimulated exudative (PEM), however, secret easily elastase,...
The Blotchy allele is one of several mutations at the Mottled locus on mouse X-chromosome. Affected males and heterozygous females within allelic groups possess varying degrees connective tissue abnormalities similar to those found in copper-deficient lathyritic animals, including aortic aneurysms reduced skin tensile strength. There evidence that defective inter- intramolecular cross-links collagen elastin account for these abnormalities. lung also structurally functionally abnormal....
Free alveolar cells obtained from healthy unstimulated hamsters were tested for their ability to form colonies in soft agar. Every bronchial washing so far contained colon-forming cells. The average plating efficiency was 8.1% (2.4-18.3%). Alveolar colony-forming characterized by having a long initial lag period (4-8 days) and only mononuclear phagocytes found the colony. Medium conditioned baby hamster kidney or other required initiation maintenance of growth. normal mice rats also formed...
The development of pulmonary fibrosis in hamsters after the intratracheal instillation bleomycin is attended by exudation polymorphonuclear leukocytes (PMNs), but function these cells unknown. In this study, we examined effect PMN depletion on lung collagen synthetic rate bleomycin-treated hamsters. Antiserum to hamster PMNs was produced rabbits and injected intraperitoneally into fourth through seventh days or saline. Control animals received either normal rabbit serum (NRS) no serum....